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The water-soluble sulfated xylogalactoarabinans from green seaweed Cladophora falklandica are constituted by a backbone of 4-linked β-L-arabinopyranose units partially sulfated mainly on C3 and also on C2. Besides, partial glycosylation mostly on C2 with single stubs of β-D-xylopyranose, or single stubs of β-D-galactofuranose or short chains comprising (1 → 5)- and/or (1 → 6)-linkages, was also found. These compounds showed anticoagulant activity, although much lower than that of heparin. The effect of a purified fraction (F1) on the fibrin network was studied in detail. It modifies the kinetics of fibrin formation, suggesting an impaired polymerization process. Scanning electron microscopy showed a laxer conformation, with larger interstitial pores than the control. Accordingly, this network was lysed more easily. These fibrin properties would reduce the time of permanence of the clot in the blood vessel, inducing a lesser thrombogenic state. One of the possible mechanisms of its anticoagulant effect is direct thrombin inhibition. © 2016 Elsevier Ltd


Documento: Artículo
Título:Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network
Autor:Arata, P.X.; Quintana, I.; Raffo, M.P.; Ciancia, M.
Filiación:Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Biología Aplicada y Alimentos, Cátedra de Química de Biomoléculas, Av. San Martín 4453Buenos Aires C1417DSE, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Laboratorio de Hemostasia y Trombosis, Ciudad Universitaria – Pabellón 2Buenos Aires C1428EHA, Argentina
Consejo Nacional de lnvestigaciones Cientificas y Tecnicas-Centro Nacional Patagónico (CENPAT-CONICET), Bvd. Brown 2915, Puerto Madryn, Chubut U9120ACD, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Orgánica, Consejo Nacional de lnvestigaciones Cientificas y Tecnicas-Centro de Investigación de Hidratos de Carbono (CIHIDECAR-CONICET), Ciudad Universitaria – Pabellón 2Buenos Aires C1428EHA, Argentina
Palabras clave:Anticoagulant activity; Cladophora; Fibrin network; Galactofuranose; Green seaweed; Sulfated arabinan; Blood vessels; Scanning electron microscopy; Anticoagulant activities; Arabinans; Cladophora; Fibrin network; Galactofuranose; Green seaweed; Seaweed; anticoagulant agent; fibrin; polysaccharide; sulfate; xylopyranose; xylose; analogs and derivatives; animal; chemistry; conformation; drug effects; fibrinolysis; green alga; human; Leporidae; metabolism; preclinical study; procedures; scanning electron microscopy; seaweed; Animals; Anticoagulants; Carbohydrate Conformation; Chlorophyta; Drug Evaluation, Preclinical; Fibrin; Fibrinolysis; Humans; Microscopy, Electron, Scanning; Polysaccharides; Rabbits; Seaweed; Sulfates; Xylose
Página de inicio:139
Página de fin:150
Título revista:Carbohydrate Polymers
Título revista abreviado:Carbohydr Polym
CAS:fibrin, 9001-31-4; sulfate, 14808-79-8; xylose, 25990-60-7, 58-86-6; Anticoagulants; Fibrin; Polysaccharides; Sulfates; xylopyranose; Xylose


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---------- APA ----------
Arata, P.X., Quintana, I., Raffo, M.P. & Ciancia, M. (2016) . Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network. Carbohydrate Polymers, 154, 139-150.
---------- CHICAGO ----------
Arata, P.X., Quintana, I., Raffo, M.P., Ciancia, M. "Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network" . Carbohydrate Polymers 154 (2016) : 139-150.
---------- MLA ----------
Arata, P.X., Quintana, I., Raffo, M.P., Ciancia, M. "Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network" . Carbohydrate Polymers, vol. 154, 2016, pp. 139-150.
---------- VANCOUVER ----------
Arata, P.X., Quintana, I., Raffo, M.P., Ciancia, M. Novel sulfated xylogalactoarabinans from green seaweed Cladophora falklandica: Chemical structure and action on the fibrin network. Carbohydr Polym. 2016;154:139-150.