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Abstract:

Molecular motors play relevant roles on the regulation of mitochondria size and shape, essential properties for the cell homeostasis. In this work, we tracked single rod-shaped mitochondria with nanometer precision to explore the performance of microtubule motor teams during processive anterograde and retrograde transport. We analyzed simultaneously the organelle size and verified that mitochondria retracted during retrograde transport with their leading tip moving slower in comparison with the rear tip. In contrast, mitochondria preserved their size during anterograde runs indicating a different performance of plus-end directed teams. These results were interpreted considering the different performance of dynein and kinesin teams and provide valuable information on the collective action of motors during mitochondria transport. © 2018 The Author(s).

Registro:

Documento: Artículo
Título:Retraction of rod-like mitochondria during microtubule-dependent transport
Autor:De Rossi, M.C.; Levi, V.; Bruno, L.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Ciudad Universitaria, Ciudad de Buenos Aires, CP1428, Argentina
Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IFIBA-CONICET, Ciudad Universitaria, Ciudad de Buenos Aires, CP1428, Argentina
Palabras clave:dynein adenosine triphosphatase; enhanced green fluorescent protein; kinesin; dynein adenosine triphosphatase; kinesin; animal cell; Article; cell organelle; cell size; cell transport; confocal microscopy; homeostasis; immortalized cell line; intracellular transport; melanophore; microtubule; mitochondrion; molecular imaging; nonhuman; Xenopus laevis; animal; genetics; metabolism; microtubule; mitochondrion; organelle shape; single cell analysis; Animals; Dyneins; Homeostasis; Kinesin; Microtubules; Mitochondria; Organelle Shape; Single-Cell Analysis; Xenopus laevis
Año:2018
Volumen:38
Número:3
DOI: http://dx.doi.org/10.1042/BSR20180208
Título revista:Bioscience Reports
Título revista abreviado:Biosci. Rep.
ISSN:01448463
CODEN:BRPTD
CAS:dynein adenosine triphosphatase; Dyneins; Kinesin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448463_v38_n3_p_DeRossi

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Citas:

---------- APA ----------
De Rossi, M.C., Levi, V. & Bruno, L. (2018) . Retraction of rod-like mitochondria during microtubule-dependent transport. Bioscience Reports, 38(3).
http://dx.doi.org/10.1042/BSR20180208
---------- CHICAGO ----------
De Rossi, M.C., Levi, V., Bruno, L. "Retraction of rod-like mitochondria during microtubule-dependent transport" . Bioscience Reports 38, no. 3 (2018).
http://dx.doi.org/10.1042/BSR20180208
---------- MLA ----------
De Rossi, M.C., Levi, V., Bruno, L. "Retraction of rod-like mitochondria during microtubule-dependent transport" . Bioscience Reports, vol. 38, no. 3, 2018.
http://dx.doi.org/10.1042/BSR20180208
---------- VANCOUVER ----------
De Rossi, M.C., Levi, V., Bruno, L. Retraction of rod-like mitochondria during microtubule-dependent transport. Biosci. Rep. 2018;38(3).
http://dx.doi.org/10.1042/BSR20180208