Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Chromaffin cell exocytosis is triggered by Ca2+ entry through several voltage-dependent channel subtypes. Because it was postulated that immediately releasable vesicles are closely associated with Ca2+ channels, we wondered what channel types are specifically coupled to the release of this pool. To study this question, cultured mouse chromaffin cell exocytosis was followed by patch-clamp membrane capacitance measurements. The immediately releasable pool was estimated using paired pulse stimulation, resulting in an upper limit of 31 ± 3 fF for control conditions (ICa: 25 ± 2 pA/pF). The N-type channel blocker ω-conotoxin-GVIA affected neither ICa nor the immediately releasable pool exocytosis; although the L channel blocker nitrendipine decreased current by 50%, it did not reduce this pool significantly; and the R channel inhibitor SNX-482 significantly reduced the current but induced only a moderate decrease in the estimated IRP exocytosis. In contrast, the P/Q channel blocker ω-Agatoxin-IVA decreased ICa by 37% but strongly reduced the immediately releasable pool (upper limit: 6 ± 1 fF). We used α1A subunit knockout mice to corroborate that P/Q Ca2+ channels were specifically linked to immediately releasable vesicles, and we found that also in this preparation the exocytosis of this pool was severely decreased (6 ± 1 fF). On the other hand, application of a strong stimulus that caused the fusion of most of releasable vesicles (3 min, 50 mM K+) induced similar exocytosis for wild type and knockout cells. Finally, whereas application of train stimulation on chromaffin cells derived from wild type mice provoked typical early synchronous and delayed asynchronous exocytosis components, the knockout derived cells presented a strongly depressed early exocytosis but showed a prominent delayed asynchronous component. These results demonstrate that P/Q are the dominant calcium channels associated to the release of immediately releasable pool in mouse chromaffin cells. © 2007 Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:P/Q Ca2+ channels are functionally coupled to exocytosis of the immediately releasable pool in mouse chromaffin cells
Autor:Álvarez, Y.D.; Ibañez, L.I.; Uchitel, O.D.; Marengo, F.D.
Filiación:Laboratorio de Fisiología y Biología Molecular, Instituto de Fisiología, Biología Molecular y Neurociencias, CONICET, Pabellón-II, (CP: 1428), Buenos Aires, Argentina
Palabras clave:Stimulus-secretion coupling; Voltage-dependent calcium channels; α1A Knockout mice; calcium channel; calcium channel blocking agent; calcium channel L type; calcium channel N type; calcium ion; conotoxin; nitrendipine; snx 482; calcium channel; calcium channel blocking agent; calcium channel P type; calcium channel Q type; animal cell; animal cell culture; article; calcium transport; cell vacuole; chromaffin cell; controlled study; exocytosis; fluorescence; knockout mouse; mouse; nonhuman; patch clamp; priority journal; stimulation; stimulus; wild type; animal; drug effect; electric capacitance; electrostimulation; metabolism; mouse mutant; physiology; Mus; Animals; Calcium Channel Blockers; Calcium Channels; Calcium Channels, P-Type; Calcium Channels, Q-Type; Chromaffin Cells; Electric Capacitance; Electric Stimulation; Exocytosis; Mice; Mice, Knockout; Patch-Clamp Techniques
Año:2008
Volumen:43
Número:2
Página de inicio:155
Página de fin:164
DOI: http://dx.doi.org/10.1016/j.ceca.2007.04.014
Título revista:Cell Calcium
Título revista abreviado:Cell Calcium
ISSN:01434160
CODEN:CECAD
CAS:calcium ion, 14127-61-8; nitrendipine, 39562-70-4; Calcium Channel Blockers; Calcium Channels; Calcium Channels, P-Type; Calcium Channels, Q-Type
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01434160_v43_n2_p155_Alvarez

Referencias:

  • Robinson, I.M., Finnegan, J.M., Monck, J.R., Wightman, R.M., Fernandez, J.M., Colocalization of calcium entry and exocytotic release sites in adrenal chromaffin cells (1995) Proc. Natl. Acad. Sci. U.S.A., 92, pp. 2474-2478
  • Augustine, G.J., Neher, E., Calcium requirements for secretion in bovine chromaffin cells (1992) J. Physiol., 450, pp. 247-271
  • Oheim, M., Kirchhoff, F., Stuhmer, W., Calcium microdomains in regulated exocytosis (2006) Cell Calcium, 40, pp. 423-439
  • Marengo, F.D., Monck, J.R., Development and dissipation of Ca(2+) gradients in adrenal chromaffin cells (2000) Biophys J., 79, pp. 1800-1820
  • Marengo, F.D., Calcium gradients and exocytosis in bovine adrenal chromaffin cells (2005) Cell Calcium, 38, pp. 87-99
  • Seward, E.P., Chernevskaya, N.I., Nowycky, M.C., Ba2+ ions evoke two kinetically distinct patterns of exocytosis in chromaffin cells, but not in neurohypophysial nerve terminals (1996) J. Neurosci., 16, pp. 1370-1379
  • Horrigan, F.T., Bookman, R.J., Releasable pools and the kinetics of exocytosis in adrenal chromaffin cells (1994) Neuron, 13, pp. 1119-1129
  • Voets, T., Neher, E., Moser, T., Mechanisms underlying phasic and sustained secretion in chromaffin cells from mouse adrenal slices (1999) Neuron, 23, pp. 607-615
  • Artalejo, C.R., Adams, M.E., Fox, A.P., Three types of Ca2+ channel trigger secretion with different efficacies in chromaffin cells (1994) Nature, 367, pp. 72-76
  • Aldea, M., Jun, K., Shin, H.S., A perforated patch-clamp study of calcium currents and exocytosis in chromaffin cells of wild-type and alpha(1A) knockout mice (2002) J. Neurochem., 81, pp. 911-921
  • Jun, K., Piedras-Renteria, E.S., Smith, S.M., Ablation of P/Q-type Ca(2+) channel currents, altered synaptic transmission, and progressive ataxia in mice lacking the alpha(1A)-subunit (1999) Proc. Natl. Acad. Sci. U.S.A., 96, pp. 15245-15250
  • Neher, E., Correction for liquid junction potentials in patch clamp experiments (1992) Methods Enzymol., 207, pp. 123-131
  • Fidler, N., Fernandez, J.M., Phase tracking: an improved phase detection technique for cell membrane capacitance measurements (1989) Biophys. J., 56, pp. 1153-1162
  • Gillis, K.D., Mossner, R., Neher, E., Protein kinase C enhances exocytosis from chromaffin cells by increasing the size of the readily releasable pool of secretory granules (1996) Neuron, 16, pp. 1209-1220
  • Artalejo, C.R., Henley, J.R., McNiven, M.A., Palfrey, H.C., Rapid endocytosis coupled to exocytosis in adrenal chromaffin cells involves Ca2+, GTP, and dynamin but not clathrin (1995) Proc. Natl. Acad. Sci. U.S.A., 92, pp. 8328-8332
  • Smith, C.B., Betz, W.J., Simultaneous independent measurement of endocytosis and exocytosis (1996) Nature, 380, pp. 531-534
  • Moser, T., Neher, E., Estimation of mean exocytic vesicle capacitance in mouse adrenal chromaffin cells (1997) Proc. Natl. Acad. Sci. U.S.A., 94, pp. 6735-6740
  • Heinemann, C., von Ruden, L., Chow, R.H., Neher, E., A two-step model of secretion control in neuroendocrine cells (1993) Pflugers Arch., 424, pp. 105-112
  • Marengo, F.D., Monck, J.R., Spatial distribution of Ca(2+) signals during repetitive depolarizing stimuli in adrenal chromaffin cells (2003) Biophys. J., 85, pp. 3397-3417
  • Engisch, K.L., Nowycky, M.C., Calcium dependence of large dense-core vesicle exocytosis evoked by calcium influx in bovine adrenal chromaffin cells (1996) J. Neurosci., 16, pp. 1359-1369
  • Chan, S.A., Polo-Parada, L., Smith, C., Action potential stimulation reveals an increased role for P/Q-calcium channel-dependent exocytosis in mouse adrenal tissue slices (2005) Arch. Biochem. Biophys., 435, pp. 65-73
  • Kim, S.J., Lim, W., Kim, J., Contribution of L- and N-type calcium currents to exocytosis in rat adrenal medullary chromaffin cells (1995) Brain Res., 675, pp. 289-296
  • Currie, K.P.M., Fox, A.P., Comparison of N- and P/Q-type voltage-gated calcium channel current inhibition (1997) J. Neurosci., 17, pp. 4570-4579
  • Lukyanetz, E.A., Neher, E., Different types of calcium channels and secretion from bovine chromaffin cells (1999) Eur. J. Neurosci., 11, pp. 2865-2873
  • Moser, T., Neher, E., Rapid exocytosis in single chromaffin cells recorded from mouse adrenal slices (1997) J. Neurosci., 17, pp. 2314-2323
  • Albillos, A., Neher, E., Moser, T., R-Type Ca2+ channels are coupled to the rapid component of secretion in mouse adrenal slice chromaffin cells (2000) J. Neurosci., 20, pp. 8323-8330
  • Lara, B., Gandia, L., Martinez-Sierra, R., Torres, A., Garcia, A.G., Q-type Ca2+ channels are located closer to secretory sites than L-type channels: functional evidence in chromaffin cells (1998) Pflugers Arch., 435, pp. 472-478
  • Segura, J., Gil, A., Soria, B., Modeling study of exocytosis in neuroendocrine cells: influence of the geometrical parameters (2000) Biophys J., 79, pp. 1771-1786
  • Klingauf, J., Neher, E., Modeling buffered Ca2+ diffusion near the membrane: implications for secretion in neuroendocrine cells (1997) Biophys. J., 72, pp. 674-690
  • Chow, R.H., Klingauf, J., Neher, E., Time course of Ca2+ concentration triggering exocytosis in neuroendocrine cells (1994) Proc. Natl. Acad. Sci. U.S.A., 91, pp. 12765-12769
  • Lopez, I., Giner, D., Ruiz-Nuno, A., Tight coupling of the t-SNARE and calcium channel microdomains in adrenomedullary slices and not in cultured chromaffin cells (2007) Cell Calcium, 41, pp. 547-558
  • Zamponi, G.W., Regulation of presynaptic calcium channels by synaptic proteins (2003) J. Pharmacol. Sci., 92, pp. 79-83
  • Harkins, A.B., Cahill, A.L., Powers, J.F., Tischler, A.S., Fox, A.P., Deletion of the synaptic protein interaction site of the N-type (CaV2.2) calcium channel inhibits secretion in mouse pheochromocytoma cells (2004) Proc. Natl. Acad. Sci. U.S.A., 101, pp. 15219-15224
  • Mochida, S., Sheng, Z.H., Baker, C., Kobayashi, H., Catterall, W.A., Inhibition of neurotransmission by peptides containing the synaptic protein interaction site of N-type Ca2+ channels (1996) Neuron, 17, pp. 781-788
  • Kamp, M.A., Krieger, A., Henry, M., Hescheler, J., Weiergraber, M., Schneider, T., Presynaptic 'Ca2.3-containing' E-type Ca channels share dual roles during neurotransmitter release (2005) Eur. J. Neurosci., 21, pp. 1617-1625
  • Arroyo, G., Aldea, M., Fuentealba, J., Albillos, A., García, A.G., SNX482 selectively blocks P/Q Ca2+ channels and delays the inactivation of Na+ channles of chromaffin cells (2003) Eur. J. Pharmacol., 475, pp. 11-18
  • Oré, L.O., Artalejo, A.R., Intracellular Ca2+ microdomain-triggered exocytosis in neuroendocrine cells (2005) Trends Neurosci., 27, pp. 113-115
  • Becherer, U., Moser, T., Stuhmer, W., Oheim, M., Calcium regulates exocytosis at the level of single vesicles (2003) Nat. Neurosci., 6, pp. 846-853
  • Wykes, R.C.E., Bauer, C., Seward, E.P., (2005) Selective inhibition of rapid exocytosis from adrenal chromaffin cells by adnoviral expression of a mutant calmodulin, , Biophysical Society 49th Annual Meeting Long Beach, CA USA 1290-POS
  • Chan, S.A., Polo-Parada, L., Landmesser, L.T., Smith, C., Adrenal chromaffin cells exhibit impaired granule trafficking in NCAM knockout mice (2005) J. Neurophysiol., 94, pp. 1037-1047
  • Currie, K.P., Fox, A.P., Differential facilitation of N- and P/Q-type calcium channels during trains of action potential-like waveforms (2002) J. Physiol., 539, pp. 419-431

Citas:

---------- APA ----------
Álvarez, Y.D., Ibañez, L.I., Uchitel, O.D. & Marengo, F.D. (2008) . P/Q Ca2+ channels are functionally coupled to exocytosis of the immediately releasable pool in mouse chromaffin cells. Cell Calcium, 43(2), 155-164.
http://dx.doi.org/10.1016/j.ceca.2007.04.014
---------- CHICAGO ----------
Álvarez, Y.D., Ibañez, L.I., Uchitel, O.D., Marengo, F.D. "P/Q Ca2+ channels are functionally coupled to exocytosis of the immediately releasable pool in mouse chromaffin cells" . Cell Calcium 43, no. 2 (2008) : 155-164.
http://dx.doi.org/10.1016/j.ceca.2007.04.014
---------- MLA ----------
Álvarez, Y.D., Ibañez, L.I., Uchitel, O.D., Marengo, F.D. "P/Q Ca2+ channels are functionally coupled to exocytosis of the immediately releasable pool in mouse chromaffin cells" . Cell Calcium, vol. 43, no. 2, 2008, pp. 155-164.
http://dx.doi.org/10.1016/j.ceca.2007.04.014
---------- VANCOUVER ----------
Álvarez, Y.D., Ibañez, L.I., Uchitel, O.D., Marengo, F.D. P/Q Ca2+ channels are functionally coupled to exocytosis of the immediately releasable pool in mouse chromaffin cells. Cell Calcium. 2008;43(2):155-164.
http://dx.doi.org/10.1016/j.ceca.2007.04.014