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Abstract:

Cell detachment and migration from the endothelium occurs during vasculogenesis and also in pathological states. Here, we use a novel approach to trigger single cell release from an endothelial monolayer by in-situ opening of adhesive, fibril-like environment using light-responsive ligands and scanning lasers. Cell escapes from the monolayer were observed on the fibril-like adhesive tracks with 3-15 μm width. The frequency of endothelial cell escapes increased monotonically with the fibril width and with the density of the light-activated adhesive ligand. Interestingly, treatment with VEGF induced cohesiveness within the cell layer, preventing cell leaks. When migrating through the tracks, cells presented body lateral reduction and nuclear deformation imposed by the line width and dependent on myosin contractility. Cell migration mode changed from mesenchymal to amoeboid-like when the adhesive tracks narrowed (≤5 μm). Moreover, cell nucleus was shrunk showing packed DNA on lines narrower than the nuclear dimensions in a mechanisms intimately associated with the stress fibers. This platform allows the detailed study of escapes and migratory transitions of cohesive cells, which are relevant processes in development and during diseases such as organ fibrosis and carcinomas. © 2015 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
Autor:Salierno, M.J.; García-Fernandez, L.; Carabelos, N.; Kiefer, K.; García, A.J.; Campo, A.D.
Filiación:Max-Planck-Institut für Polymerforschung, Ackermannweg 10, Mainz, 55128, Germany
Department of Biological Chemistry, School of Sciences, University of Buenos Aires, IQUIBICEN-CONICET, CABA, Argentina
Woodruff School of Mechanical Engineering and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, United States
INM - Leibniz Institute for New Materials, Campus D2 2, Saarbrücken, 66123, Germany
Saarland University, Campus Saarbrücken D2 2, Saarbrücken, 66123, Germany
Palabras clave:Cell migration; Cyclic RGD; Endothelial transition; Photoactive materials; Cytology; Endothelial cells; Ligands; Monolayers; Adhesive ligands; Cell detachment; Cell migration; Cyclic RGD; Endothelial transition; Nuclear deformations; Photoactive materials; Scanning lasers; Cells; adhesive ligand; blebbistatin; ligand; myosin; myosin adenosine triphosphatase; unclassified drug; vasculotropin; arginyl-glycyl-aspartic acid; oligopeptide; Article; cell adhesion; cell body; cell density; cell detachment; cell fate; cell interaction; cell migration; cell motion; cell nucleus; cell polarity; cell proliferation; cell shape; controlled study; endothelium cell; focal adhesion; lamellipodium; light; muscle contractility; photoactivation; priority journal; adhesion; cell culture; cell motion; chemistry; cytology; endothelium; endothelium cell; extracellular matrix; human; metabolism; physiology; radiation response; tumor microenvironment; Adhesiveness; Cell Adhesion; Cell Movement; Cells, Cultured; Cellular Microenvironment; Endothelial Cells; Endothelium; Extracellular Matrix; Humans; Light; Oligopeptides
Año:2016
Volumen:82
Página de inicio:113
Página de fin:123
DOI: http://dx.doi.org/10.1016/j.biomaterials.2015.12.001
Título revista:Biomaterials
Título revista abreviado:Biomaterials
ISSN:01429612
CODEN:BIMAD
CAS:myosin adenosine triphosphatase; vasculotropin, 127464-60-2; arginyl-glycyl-aspartic acid; Oligopeptides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01429612_v82_n_p113_Salierno

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Citas:

---------- APA ----------
Salierno, M.J., García-Fernandez, L., Carabelos, N., Kiefer, K., García, A.J. & Campo, A.D. (2016) . Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers. Biomaterials, 82, 113-123.
http://dx.doi.org/10.1016/j.biomaterials.2015.12.001
---------- CHICAGO ----------
Salierno, M.J., García-Fernandez, L., Carabelos, N., Kiefer, K., García, A.J., Campo, A.D. "Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers" . Biomaterials 82 (2016) : 113-123.
http://dx.doi.org/10.1016/j.biomaterials.2015.12.001
---------- MLA ----------
Salierno, M.J., García-Fernandez, L., Carabelos, N., Kiefer, K., García, A.J., Campo, A.D. "Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers" . Biomaterials, vol. 82, 2016, pp. 113-123.
http://dx.doi.org/10.1016/j.biomaterials.2015.12.001
---------- VANCOUVER ----------
Salierno, M.J., García-Fernandez, L., Carabelos, N., Kiefer, K., García, A.J., Campo, A.D. Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers. Biomaterials. 2016;82:113-123.
http://dx.doi.org/10.1016/j.biomaterials.2015.12.001