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We investigated the selective predation of mixotrophic and heterotrophic flagellates (MF and HF) on different heterotrophic prokaryote phylotypes (HPP; Bacteria + Archaea) living in natural assemblages from oligotrophic Antarctic lakes. In situ prey preference was analyzed for the first time on different mixotrophic taxa (Pseudopedinella sp., Ochromonas-like cells, Chrysophyceae >5 μm). The relative abundances of seven different HPP hybridized by CARD-FISH (catalyzed reporter deposition-fluorescent in situ hybridization) in natural community were compared with the proportions of hybridized cells inside digestive vacuoles. Our results showed some general trends to selectivity over some HPP. Alphaproteobacteria and Betaproteobacteria were the most abundant groups, and strikingly, a negative selection trend was detected in most samples by all bacterivorous protists. In contrast, for Actinobacteria a positive selection trend was observed in most samples, whereas Bacteroidetes seemed to be randomly preyed upon. Interestingly, similar prey preferences were observed in all bacterivorous flagellates. Our results suggest that phylogenetic affiliation determines part of the process of prey selection by protists in these lakes. Nevertheless, other features, such as cell size, morphology and the presence of the S-layer, might also significantly contribute to prey selectivity on the HPP. © 2012 The Author 2012. Published by Oxford University Press. All rights reserved.


Documento: Artículo
Título:In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content
Autor:Gerea, M.; Queimaliños, C.; Schiaffino, M.R.; Izaguirre, I.; Forn, I.; Massana, R.; Unrein, F.
Filiación:Laboratorio de Fotobiología, INIBIOMA (Instituto Investigaciones en Biodiversidad y Medio Ambiente), UNComahue-CONICET, Quintral 1250, Bariloche R8400FRF, Argentina
Consejo Nacional de Investigación Científica y Técnica (CONICET), Buenos Aires, Argentina
Departamento de Ecología Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Buenos Aires C1428EHA, Argentina
Departamento de Biología Marina y Oceanografía, Institut de Ciències Del Mar, CSIC, Barcelona 08003, Spain
Laboratorio de Ecología y Fotobiología Acuática, IIB-INTECH, Camino Circunvalación Laguna Km 6, CC 164, Chascomús 7130, Argentina
Palabras clave:CARD-FISH; Heterotrophic flagellates; Mixotrophic flagellates; Oligotrophic lakes; Prey selection; catalysis; digestive system; flagellate; heterotrophy; lake ecosystem; mixotrophy; morphology; oligotrophic environment; phylogenetics; phytoplankton; predation; prey selection; prokaryote; protist; Antarctica; Actinobacteria; Alphaproteobacteria; Archaea; Bacteria (microorganisms); Bacteroidetes; Betaproteobacteria; Chrysophyceae; Mastigophora (flagellates); Ochromonas; Prokaryota; Protista; Pseudopedinella
Página de inicio:201
Página de fin:212
Título revista:Journal of Plankton Research
Título revista abreviado:J. Plankton Res.


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---------- APA ----------
Gerea, M., Queimaliños, C., Schiaffino, M.R., Izaguirre, I., Forn, I., Massana, R. & Unrein, F. (2013) . In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content. Journal of Plankton Research, 35(1), 201-212.
---------- CHICAGO ----------
Gerea, M., Queimaliños, C., Schiaffino, M.R., Izaguirre, I., Forn, I., Massana, R., et al. "In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content" . Journal of Plankton Research 35, no. 1 (2013) : 201-212.
---------- MLA ----------
Gerea, M., Queimaliños, C., Schiaffino, M.R., Izaguirre, I., Forn, I., Massana, R., et al. "In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content" . Journal of Plankton Research, vol. 35, no. 1, 2013, pp. 201-212.
---------- VANCOUVER ----------
Gerea, M., Queimaliños, C., Schiaffino, M.R., Izaguirre, I., Forn, I., Massana, R., et al. In situ prey selection of mixotrophic and heterotrophic flagellates in Antarctic oligotrophic lakes: An analysis of the digestive vacuole content. J. Plankton Res. 2013;35(1):201-212.