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Abstract:

Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope. Among other important characteristics S-layer from Lactobacillus acidophilus binds to the cellular receptor DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin; CD209), which is involved in adhesion and infection of several families of bacteria. In this report we investigate the activity of new S-layer proteins from the Lactobacillus family (Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus helveticus and Lactobacillus kefiri) over the infection of representative microorganisms important to human health. After the treatment of DC-SIGN expressing cells with these proteins, we were able to diminish bacterial infection by up to 79% in both gram negative and mycobacterial models. We discovered that pre-treatment of the bacteria with S-layers from Lactobacillus acidophilus and Lactobacillus brevis reduced bacteria viability but also prevent infection by the pathogenic bacteria. We also proved the importance of the glycosylation of the S-layer from Lactobacillus kefiri in the binding to the receptor and thus inhibition of infection. This novel characteristic of the S-layers proteins may contribute to the already reported pathogen exclusion activity for these Lactobacillus probiotic strains; and might be also considered as a novel enzymatic antimicrobial agents to inhibit bacterial infection and entry to host cells. © 2016 Elsevier B.V.

Registro:

Documento: Artículo
Título:S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor
Autor:Prado Acosta, M.; Ruzal, S.M.; Cordo, S.M.
Filiación:Laboratorio de Bacterias Gram Positivas, Departamento de Química Biológica-IQUIBICEN, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires-CONICET, Argentina
Laboratorio de Virología, Departamento de Química Biológica-IQUIBICEN, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires-CONICET, Argentina
Palabras clave:DC-SIGN; Lactobacillus; S-layer; bacterial protein; CD209 antigen; s layer protein; unclassified drug; antiinfective agent; cell adhesion molecule; cell surface receptor; DC-specific ICAM-3 grabbing nonintegrin; lectin; membrane protein; S-layer proteins; Article; bacterial strain; bacterial viability; bacterium adherence; controlled study; enzyme inhibition; human; human cell; in vivo study; Lactobacillus; Lactobacillus acidophilus; Lactobacillus brevis; Lactobacillus helveticus; Lactobacillus kefiri; nonhuman; protein function; protein glycosylation; protein protein interaction; 3T3 cell line; animal; Bacterial Infections; chemistry; drug effects; glycosylation; metabolism; mouse; 3T3 Cells; Animals; Anti-Bacterial Agents; Bacterial Adhesion; Bacterial Infections; Cell Adhesion Molecules; Glycosylation; Humans; Lactobacillus; Lectins, C-Type; Membrane Glycoproteins; Mice; Receptors, Cell Surface
Año:2016
Volumen:92
Página de inicio:998
Página de fin:1005
DOI: http://dx.doi.org/10.1016/j.ijbiomac.2016.07.096
Título revista:International Journal of Biological Macromolecules
Título revista abreviado:Int. J. Biol. Macromol.
ISSN:01418130
CODEN:IJBMD
CAS:Anti-Bacterial Agents; Cell Adhesion Molecules; DC-specific ICAM-3 grabbing nonintegrin; Lectins, C-Type; Membrane Glycoproteins; Receptors, Cell Surface; S-layer proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v92_n_p998_PradoAcosta

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Citas:

---------- APA ----------
Prado Acosta, M., Ruzal, S.M. & Cordo, S.M. (2016) . S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor. International Journal of Biological Macromolecules, 92, 998-1005.
http://dx.doi.org/10.1016/j.ijbiomac.2016.07.096
---------- CHICAGO ----------
Prado Acosta, M., Ruzal, S.M., Cordo, S.M. "S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor" . International Journal of Biological Macromolecules 92 (2016) : 998-1005.
http://dx.doi.org/10.1016/j.ijbiomac.2016.07.096
---------- MLA ----------
Prado Acosta, M., Ruzal, S.M., Cordo, S.M. "S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor" . International Journal of Biological Macromolecules, vol. 92, 2016, pp. 998-1005.
http://dx.doi.org/10.1016/j.ijbiomac.2016.07.096
---------- VANCOUVER ----------
Prado Acosta, M., Ruzal, S.M., Cordo, S.M. S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor. Int. J. Biol. Macromol. 2016;92:998-1005.
http://dx.doi.org/10.1016/j.ijbiomac.2016.07.096