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Abstract:

The effect of the combined use of chitosan, nisin and sodium lactate on the growth of Listeria innocua, Shewanella putrefaciens and psychrophilic bacteria isolated from fish was investigated in broth by means of minimum inhibitory concentrations (MIC). Furthermore, the sites of cell-injury caused by mentioned antimicrobials and their combinations on L. innocua and S. putrefaciens were studied. MIC of antimicrobial mixtures were evaluated by Berembaum design and check board method. Antimicrobials' sites of injury were investigated by the evaluation of cell constituents' release, cell surface hydrophobicity and differential scanning calorimetry. Results depended on antimicrobial used; several combinations inhibited the growth of L. innocua and S. putrefaciens and all combinations inhibited psychrophilic bacteria. Besides, some mixtures showed synergistic effects. All the mixtures affected ribosomes and DNA of the studied bacteria. Regarding cellular envelope, antimicrobials acted according to the structural characteristics of target microorganisms. Cell damage was higher when antimicrobials were combined, which could explain the observed synergistic effects. This study demonstrates and justifies the synergistic action of chitosan, nisin and sodium lactate on the inhibition of microorganisms related to fish spoilage and remarks the promissory use of the synergic combination of antimicrobials for fish preservation. © 2015 Elsevier B.V.

Registro:

Documento: Artículo
Título:Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate
Autor:Schelegueda, L.I.; Zalazar, A.L.; Gliemmo, M.F.; Campos, C.A.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Palabras clave:Chitosan; Nisin; Sodium lactate; bacterial DNA; chitosan; lactate sodium; nisin; antiinfective agent; chitosan; lactate sodium; nisin; animal cell; Article; bacterial cell; bacterial flora; bacterial growth; bacterial membrane; bacterial structures; cell damage; cell surface; chemical interaction; controlled study; differential scanning calorimetry; fish; food preservation; food spoilage; growth inhibition; hydrophobicity; Listeria innocua; minimum inhibitory concentration; nonhuman; psychrophilic bacterium; ribosome; Shewanella putrefaciens; animal; bacterium; chemical phenomena; cytology; drug effects; drug interaction; growth, development and aging; metabolism; microbial sensitivity test; microbiology; Animals; Anti-Bacterial Agents; Bacteria; Chitosan; Drug Interactions; Fishes; Hydrophobic and Hydrophilic Interactions; Microbial Sensitivity Tests; Nisin; Sodium Lactate
Año:2016
Volumen:83
Página de inicio:396
Página de fin:402
DOI: http://dx.doi.org/10.1016/j.ijbiomac.2015.11.033
Título revista:International Journal of Biological Macromolecules
Título revista abreviado:Int. J. Biol. Macromol.
ISSN:01418130
CODEN:IJBMD
CAS:chitosan, 9012-76-4; lactate sodium, 72-17-3; nisin, 1414-45-5; Anti-Bacterial Agents; Chitosan; Nisin; Sodium Lactate
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v83_n_p396_Schelegueda

Referencias:

  • Pascual-Anderson, M.R., Microbiología alimentaria (2000) Metodología para alimentos y bebidas, , Díaz de Santos S.A., Madrid, Spain
  • Gram, L., Microbiological spoilage of fish and seafood products (2010) Compendium of the Microbiological Spoilage of Foods and Beverages, , Springer, New York, USA, W.H. Sperber, M.P. Doyle (Eds.)
  • Orsi, R.H., den Bakker, H.C., Wiedmann, M., Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics (2011) Int. J. Med. Microbiol., 301, pp. 79-96
  • Chang, Y.H., Gu, W.M., McLandsborough, L., Low concentration of ethylenediaminetetraacetic acid (EDTA) affects biofilm formation of Listeria monocytogenes by inhibiting its initial adherence (2012) Food Microbiol., 29 (1), pp. 10-17
  • The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2010 (2012) Eur. Food Saf. Authority J., 10 (3), pp. 1-442. , 2597
  • Gram, L., Dalgaard, P., Fish spoilage bacteria - problems and solutions (2002) Curr. Opin. Biotech., 13, pp. 262-266
  • Skjerdal, O.T., Lorentzen, G., Tryland, I., Berg, J.D., New method for rapid and sensitive quantification of sulphide-producing bacteria in fish from arctic and temperate waters (2004) Int. J. Food Microbiol., 93 (3), pp. 325-333
  • Bagge, D., Hjelm, M., Johansen, C., Huber, I., Gram, L., Shewanella putrefaciens adhesion and biofilm formation on food processing surfaces (2001) Appl. Environ. Microbiol., 67 (5), pp. 2319-2325
  • Pagani, L., Lang, A., Vedovelli, C., Moling, O., Rimenti, G., Pristerà, R., Mian, P., Soft tissue infection and bacteremia caused by Shewanella putrefaciens (2003) J. Clin. Microbiol., 41 (5), pp. 2240-2241
  • Campos, C.A., Castro, M.P., Gliemmo, M.F., Schelegueda, L.I., Use of natural antimicrobials for the control of Listeria monocytogenes in foods (2011) Science Against Microbial Pathogens: Communicating Current Research And Technological Advances, pp. 1112-1123. , Formatex, Badajoz, Spain, A. Méndez-Vilas (Ed.)
  • Campos, C.A., Castro, M.P., Aubourg, S., Barros Velazquez, J., Use of natural preservatives in seafood (2011) Novel Technologies in Food Science - Their Impact On Products, Consumer Trends And Environment, , Springer, New York, USA, A. McElhatton, P. Sobral (Eds.)
  • Ghaly, A.E., Dave, D., Budge, S., Brooks, M.S., Fish spoilage mechanisms and preservation techniques: review (2010) Am. J. Appl. Sci., 7 (7), pp. 859-877
  • Schelegueda, L.I., Gliemmo, M.F., Campos, C.A., Antimicrobial synergic effect of chitosan with sodium lactate, nisin or potassium sorbate against the bacterial flora of fish (2012) J. Food Res., 1 (3), pp. 272-281
  • Newton, B.A., The release of soluble constituents from washed cells of Pseudomonas aeruginosa by the action of polymyxin (1958) J. Gen. Microbiol., 9, pp. 54-64
  • Carson, C.F., Mee, B.J., Riley, T.V., Mechanism of action of Melaleuca alternifolia (Tea Tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy (2002) Antimicrob. Agents Chemother., 46, pp. 1914-1920
  • Ming, X., Daeschel, M.A., Correlation of cellular phospholipid content with nisin resistance of Listeria monocytogenes Scott A (1994) J. Food Protect., 58 (4), pp. 416-420
  • Lee, J., Kaletunc, G., Evaluation of the heat inactivation of Escherichia coli and Lactobacillus plantarum by differential scanning calorimetry (2002) Appl. Environ. Microbiol., 68 (11), pp. 5379-5386
  • Lee, J., Kaletunc, G., Evaluation by differential scanning calorimetry of the effect of acid, ethanol and NaCl on Escherichia coli (2005) J. Food Protect., 68 (3), pp. 487-493
  • Friedly, E.C., Crandall, P.G., Ricke, S., ÓBryan, C.A., Martin, E.M., Boyd, L.M., Identification of Listeria innocua surrogates for Listeria monocytogenes in hamburger patties (2008) J. Food Sci., 73, pp. 174-178
  • Rodriguez, O., Velázquez, J.B., Ojea, A., Piñeiro, C., Aubourg, S.P., Evaluation of sensory and microbiological changes and identification of proteolytic bacteria during the iced storage of farmed turbot (Psetta maxima) (2003) J. Food Sci., 68, pp. 2764-2770
  • Berembaum, M.C., A method for testing for synergy with any number of agents (1978) J. Infect. Dis., 137 (2), pp. 122-130
  • López-Malo Vigil, A., Palou, E., Parish, M.E., Davidson, P.M., Methods for activity assay and evaluation of results (2005) Antimicrobials in Foods, pp. 659-680. , CRC Press Taylor and Francis Group, Boca Ratón, Florida, USA, P.M. Davidson, J.N. Sofos, A.L. Branen (Eds.)
  • Gliemmo, M.F., Schelegueda, L.I., Gerschenson, L.N., Campos, C.A., Effect of aspartame and other additives on the growth and thermal inactivation of Zygosaccharomyces bailii in acidified aqueous systems (2013) Food Res. Int., 53, pp. 209-217
  • Bani-Jaber, A., McGuire, J., Ayres, J.W., Daeschel, M.A., Efficacy of the antimicrobial peptide nisin in emulsifying oil in water (2000) J. Food Sci., 65 (3), pp. 502-506
  • Schelegueda, L.I., Vallejo, M., Gliemmo, M.F., Marguet, E.R., Campos, C.A., Synergistic antimicrobial action and potential application for fish preservation of a bacteriocin produced by Enterococcus mundtii isolated from Odontesthes platensis (2015) LWT - Food Sci. Technol., 64, pp. 794-801
  • No, H.K., Young, P.N., Lee, S.H., Meyers, S.P., Antibacterial activity of chitosans and chitosan oligomers with different molecular weights (2002) Int. J. Food Microbiol., 74 (1-2), pp. 65-72
  • Helander, I.M., Nurmiaho-Lassila, E.L., Ahvenainen, R., Rhoades, J., Roller, S., Chitosan disrupst the barrier properties of the outer membrane of Gram-negative bacteria (2001) Int. J. Food Microobiol., 71 (2-3), pp. 235-244
  • No, H.K., Meyers, S.P., Prinyawiwatkul, W., Xu, Z., Applications of chitosan for improvement of quality and shelf life of foods: a review (2007) J. Food Sci., 72 (5), pp. 87-100
  • Adams, M., Nisin in multifactorial food preservation (2003) Natural Antimicrobials for the Minimal Processing of Food, , Woodhead Publishing Limited-CRC Press LLC, Cambridge, England, S. Roller (Ed.)
  • Thomas, L.V., Clarkson, M.R., Delves-Broughton, J., Nisin, Natural Food Antimicrobial Systems (2000), pp. 463-524. , CRC Press Inc, Boca Ratón, Florida, USA, A.S. Naidú (Ed.); Abou-Zeid, K.A., Yoon, K.S., Oscar, T.P., Schwarz, J.G., Hashem, F.M., Whiting, R.C., Survival and growth of Listeria monocytogenes in broth as a function of temperature, pH, and potassium lactate and sodium diacetate concentrations (2007) J. Food Protect., 70, pp. 2620-2625
  • Alakomi, H.L., Skyttä, E., Saarela, M., Mattila-Sandholm, T., Latva-Kala, K., Helander, I.M., Lactic acid permeabilizes Gram-negative bacteria by disrupting the outer membrane (2000) Appl. Environ. Microbiol., 66 (5), pp. 2001-2005
  • Thomas, L.V., Delves-Broughton Hoover, D.G., Nisin, Antimicrobials in Foods (2005), pp. 237-274. , CRC Press Taylor-Francis Group, Boca Ratón, Florida, USA, P.M. Davidson, J.N. Sofos, A.L. Branen (Eds.); Cai, J., Yang, J., Wang, C., Hu, Y., Lin, J., Fan, L., Structural characterization and antimicrobial activity of chitosan (CS-40)/nisin complexes (2010) J. Appl. Polym. Sci., 116, pp. 3702-3707

Citas:

---------- APA ----------
Schelegueda, L.I., Zalazar, A.L., Gliemmo, M.F. & Campos, C.A. (2016) . Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate. International Journal of Biological Macromolecules, 83, 396-402.
http://dx.doi.org/10.1016/j.ijbiomac.2015.11.033
---------- CHICAGO ----------
Schelegueda, L.I., Zalazar, A.L., Gliemmo, M.F., Campos, C.A. "Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate" . International Journal of Biological Macromolecules 83 (2016) : 396-402.
http://dx.doi.org/10.1016/j.ijbiomac.2015.11.033
---------- MLA ----------
Schelegueda, L.I., Zalazar, A.L., Gliemmo, M.F., Campos, C.A. "Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate" . International Journal of Biological Macromolecules, vol. 83, 2016, pp. 396-402.
http://dx.doi.org/10.1016/j.ijbiomac.2015.11.033
---------- VANCOUVER ----------
Schelegueda, L.I., Zalazar, A.L., Gliemmo, M.F., Campos, C.A. Inhibitory effect and cell damage on bacterial flora of fish caused by chitosan, nisin and sodium lactate. Int. J. Biol. Macromol. 2016;83:396-402.
http://dx.doi.org/10.1016/j.ijbiomac.2015.11.033