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Mandal, P.; Pujol, C.A.; Damonte, E.B.; Ghosh, T.; Ray, B. "Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity" (2010) International Journal of Biological Macromolecules. 46(2):173-178
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Abstract:

Natural compounds offer interesting pharmacological perspectives for antiviral drug development with regard to broad-spectrum antiviral properties and novel modes of action. In this study, we have analyzed alkali-extracted xylan of Scinaia hatei. Alkali extraction of this red alga yielded a xylan shown to have a molecular mass of 120kDa and a linear structure of (1→4)-linked β-d-xylopyranosyl residues. Derivatives (S1, S2, S3 and S4) generated by chemical sulfation from this macromolecule had degree of sulfation between 0.93 and 1.95, and contained strong anti-HSV activity with inhibitory concentration 50% (IC50) from 0.22 to 1.37μg/ml. Furthermore, they had no direct inactivating effect on virions in a virucidal assay. Sulfate groups account for their in vitro antiviral activity. Interestingly, sulfated xylans already exerted anti-HSV activity when only pre-incubated with the cultured cells prior to infection, thus pointing to a main inhibitory effect on viral entry. © 2009 Elsevier B.V.

Registro:

Documento: Artículo
Título:Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity
Autor:Mandal, P.; Pujol, C.A.; Damonte, E.B.; Ghosh, T.; Ray, B.
Filiación:Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan, WB 713104, India
Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales UBA, Ciudad Universitaria-Pabellón 2 Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Antiviral activity; Herpes simplex virus; Liagoraceae; Scinaia hatei; Sulfation; Xylan; acetone; antivirus agent; petroleum ether; polysaccharide sulfate; xylan; animal cell; antiviral activity; article; chemical bond; controlled study; drug cytotoxicity; drug structure; gel permeation chromatography; glycosidic linkage; Herpes simplex virus; Herpes simplex virus 1; Herpes simplex virus 2; macromolecule; molecular weight; nonhuman; proton nuclear magnetic resonance; Scinaia hatei; seaweed; solvent extraction; Soxhlet extraction; structure activity relation; sulfation; virion; virus inactivation; Algae, Red; Animals; Antiviral Agents; Cercopithecus aethiops; Chromatography, Gel; Glycosides; Herpesvirus 1, Human; Herpesvirus 2, Human; Magnetic Resonance Spectroscopy; Molecular Weight; Sulfur; Vero Cells; Xylans; Liagoraceae; Rhodophyta; Scinaia; Simplexvirus
Año:2010
Volumen:46
Número:2
Página de inicio:173
Página de fin:178
DOI: http://dx.doi.org/10.1016/j.ijbiomac.2009.12.003
Título revista:International Journal of Biological Macromolecules
Título revista abreviado:Int. J. Biol. Macromol.
ISSN:01418130
CODEN:IJBMD
CAS:acetone, 67-64-1; petroleum ether, 8032-32-4; polysaccharide sulfate, 79933-28-1; xylan, 9014-63-5; Antiviral Agents; Glycosides; Sulfur, 7704-34-9; Xylans
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v46_n2_p173_Mandal

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Citas:

---------- APA ----------
Mandal, P., Pujol, C.A., Damonte, E.B., Ghosh, T. & Ray, B. (2010) . Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity. International Journal of Biological Macromolecules, 46(2), 173-178.
http://dx.doi.org/10.1016/j.ijbiomac.2009.12.003
---------- CHICAGO ----------
Mandal, P., Pujol, C.A., Damonte, E.B., Ghosh, T., Ray, B. "Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity" . International Journal of Biological Macromolecules 46, no. 2 (2010) : 173-178.
http://dx.doi.org/10.1016/j.ijbiomac.2009.12.003
---------- MLA ----------
Mandal, P., Pujol, C.A., Damonte, E.B., Ghosh, T., Ray, B. "Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity" . International Journal of Biological Macromolecules, vol. 46, no. 2, 2010, pp. 173-178.
http://dx.doi.org/10.1016/j.ijbiomac.2009.12.003
---------- VANCOUVER ----------
Mandal, P., Pujol, C.A., Damonte, E.B., Ghosh, T., Ray, B. Xylans from Scinaia hatei: Structural features, sulfation and anti-HSV activity. Int. J. Biol. Macromol. 2010;46(2):173-178.
http://dx.doi.org/10.1016/j.ijbiomac.2009.12.003