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Abstract:

In order to determine the structure of the fibrillar cell wall, the material isolated from cystocarpic thalli of the red seaweed Iridaea undulosa was fractionated using different media. While classical methods produced a scarce solubilization of material, the use of lithium salts in polar aprotic solvents (dimethylsulfoxide or N,N-dimethylacetamide), had successfully extracted higher amounts of material. The final residue from the Li+/DMSO extraction contains cellulose and a mannan, while that from the Li+/DMAc extraction contains only cellulose and traces of a galactan. Methylation analyses of both residues confirm the presence of those polysaccharides, and shows that the mannan is (1→4)-linked. Treatment with proteases suggests that the protein is efficiently shielded from digestion. All the extracts and residues contain major amounts of (glyco)proteins and/or proteins, in agreement with a previous suggestion that they are of major importance in the structure of the cell wall. Copyright (C) 2000 Elsevier Science B.V.

Registro:

Documento: Artículo
Título:Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides
Autor:Flores, M.L.; Stortz, C.A.; Cerezo, A.S.
Filiación:Farmacognosia, Depto. Farmacia, Fac. Cie. Nat., U., Comodoro Rivadavia, Argentina
Depto. de Quim. Organ.-CIHIDECAR, Fac. Cie. Exact. Y Nat., Univ. B., Buenos Aires, Argentina
Palabras clave:Cell wall; Fibrillar polysaccharides; Iridaea undulosa; Rhodophyta; cellulose; dimethyl sulfoxide; galactan; glycoprotein; lithium salt; mannan; n,n dimethylacetamide; polysaccharide; protein; proteinase; reagent; solvent; analytic method; article; cell wall; chemical analysis; controlled study; extraction; fractionation; iridaea undulosa; methylation; nonhuman; seaweed; solubilization; Acetylglucosamine; Cell Extracts; Cell Fractionation; Cell Wall; Methylation; Monosaccharides; Plant Proteins; Polysaccharides; Seaweed
Año:2000
Volumen:27
Número:1
Página de inicio:21
Página de fin:27
DOI: http://dx.doi.org/10.1016/S0141-8130(99)00116-6
Título revista:International Journal of Biological Macromolecules
Título revista abreviado:Int. J. Biol. Macromol.
ISSN:01418130
CODEN:IJBMD
CAS:Acetylglucosamine, 7512-17-6; Cell Extracts; Monosaccharides; Plant Proteins; Polysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01418130_v27_n1_p21_Flores

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Citas:

---------- APA ----------
Flores, M.L., Stortz, C.A. & Cerezo, A.S. (2000) . Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides. International Journal of Biological Macromolecules, 27(1), 21-27.
http://dx.doi.org/10.1016/S0141-8130(99)00116-6
---------- CHICAGO ----------
Flores, M.L., Stortz, C.A., Cerezo, A.S. "Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides" . International Journal of Biological Macromolecules 27, no. 1 (2000) : 21-27.
http://dx.doi.org/10.1016/S0141-8130(99)00116-6
---------- MLA ----------
Flores, M.L., Stortz, C.A., Cerezo, A.S. "Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides" . International Journal of Biological Macromolecules, vol. 27, no. 1, 2000, pp. 21-27.
http://dx.doi.org/10.1016/S0141-8130(99)00116-6
---------- VANCOUVER ----------
Flores, M.L., Stortz, C.A., Cerezo, A.S. Studies on the skeletal cell wall of the cystocarpic stage of the red seaweed Iridaea undulosa B.Part II. Fractionation of the cell wall and methylation analysis of the inner core-fibrillar polysaccharides. Int. J. Biol. Macromol. 2000;27(1):21-27.
http://dx.doi.org/10.1016/S0141-8130(99)00116-6