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Abstract:

Base-labile 6-chloro-2′,3′,5′-tri-O-acetylpurine riboside (1c) and 2-amino-6-chloro-2′,3′,5′-tri-O-acetylpurine riboside (1d) were fully deacetylated through Candida antarctica B lipase hydrolysis, affording respectively 6-chloropurine riboside (2c) and 2-amino-6-chloro-purine riboside (2d). Quantitative results were found at pH 7 and 60 °C in 24 h for 1c and 72 h for 1d. This mild and simple enzymatic technique represents a convenient procedure for the removal of acetyl groups from such base labile halogenated nucleosides.

Registro:

Documento: Artículo
Título:A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides
Autor:Roncaglia, D.I.; Schmidt, A.M.; Iglesias, L.E.; Iribarren, A.M.
Filiación:Centro de Estudios e Investigaciones, Universidad Nacional de Quilmes, Roque Sáenz Peña 180, (1876) Bernal, Argentina
INGEBI(CONICET), Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:2-amino-6-chloropurine riboside; 6-chloropurine riboside; Deacetylation; Lipases; Nucleosides; 2 amino 6 chloropurine riboside; 6 chloropurine ribonucleoside; nebularine; nucleoside; purine derivative; purine nucleoside; triacylglycerol lipase; unclassified drug; article; Aspergillus; Burkholderia cepacia; Candida; catalysis; enzyme activity; hydrolysis; Mucor; nonhuman; thin layer chromatography
Año:2001
Volumen:23
Número:17
Página de inicio:1439
Página de fin:1443
DOI: http://dx.doi.org/10.1023/A:1011615224704
Título revista:Biotechnology Letters
Título revista abreviado:Biotechnol. Lett.
ISSN:01415492
CODEN:BILED
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01415492_v23_n17_p1439_Roncaglia

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  • Zemlicka, J., Sorm, F., Nucleic acids components and their analogs. The reaction of dimerhylchloromethyleneammonium chloride with 2′,3′,5′-tri-O-acetylinosine. A new synthesis of 6-chloro 9-(β-D-ribofuranosyl)purine (1965) Collect. Czech. Chem. Commun., 30, pp. 1880-1889

Citas:

---------- APA ----------
Roncaglia, D.I., Schmidt, A.M., Iglesias, L.E. & Iribarren, A.M. (2001) . A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides. Biotechnology Letters, 23(17), 1439-1443.
http://dx.doi.org/10.1023/A:1011615224704
---------- CHICAGO ----------
Roncaglia, D.I., Schmidt, A.M., Iglesias, L.E., Iribarren, A.M. "A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides" . Biotechnology Letters 23, no. 17 (2001) : 1439-1443.
http://dx.doi.org/10.1023/A:1011615224704
---------- MLA ----------
Roncaglia, D.I., Schmidt, A.M., Iglesias, L.E., Iribarren, A.M. "A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides" . Biotechnology Letters, vol. 23, no. 17, 2001, pp. 1439-1443.
http://dx.doi.org/10.1023/A:1011615224704
---------- VANCOUVER ----------
Roncaglia, D.I., Schmidt, A.M., Iglesias, L.E., Iribarren, A.M. A simple and efficient enzymatic procedure for the deprotection of two base labile chlorinated purine ribosides. Biotechnol. Lett. 2001;23(17):1439-1443.
http://dx.doi.org/10.1023/A:1011615224704