Artículo
Bellani, M.; Nudel, C.; Sanchez Rivas, C. "Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli" (1997) Biotechnology Letters. 19(4):331-333
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Abstract:
The resolution system from Tn 1000 was cloned into a pBR322 recombinant plasmid carrying E. coli threonine operon. The resulting plasmid displayed a significant increase in segregational stability with respect to the original plasmid, either in a rec+ or a rec- E. coli strain (100% of plasmid-containing cells versus 64% and 1% respectively). The Tn 1000 resolution system proved to be responsible for the stabilising effect exerted by the transposon when present in recombinant plasmids.
Registro:
| Documento: | Artículo |
| Título: | Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli |
| Autor: | Bellani, M.; Nudel, C.; Sanchez Rivas, C. |
| Filiación: | Fac. de Ciencias Exactas y Naturales, Depto. Quim. Biológica, Ciudad Universitaria, Buenos Aires, Argentina Fac. de Farmacia y Bioquímica, Biotecnología, Universidad de Buenos Aires, Junín 956 (1113), Buenos Aires, Argentina |
| Palabras clave: | threonine; article; cloning; escherichia coli; operon; recombinant plasmid; transposon |
| Año: | 1997 |
| Volumen: | 19 |
| Número: | 4 |
| Página de inicio: | 331 |
| Página de fin: | 333 |
| DOI: | http://dx.doi.org/10.1023/A:1018394616153 |
| Título revista: | Biotechnology Letters |
| Título revista abreviado: | BIOTECHNOL. LETT. |
| ISSN: | 01415492 |
| CODEN: | BILED |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01415492_v19_n4_p331_Bellani |
Referencias:
- Austin, S., Ziese, M., Sternberg, N., (1981) Cell, 25, pp. 729-736
- Bellani, M., Schlein, V., Nudel, C., Sanchez Rivas, C., (1997) Biotechnol. Lett., 19, pp. 1-5
- Birnboim, H.C., Doly, J., (1979) Nucleic Acids Res., 7, pp. 1513-1523
- Broom, J.E., Hill, D.F., Hughes, G., Jones, W.A., McNaughton, J.C., Stockwell, P.A., Petersen, G.B., (1991) Sequence of a Transposon Identified as Tn1000, , EMBL/GenBank/DDBJ Accesion Number. X60200
- Dillon, J.-A.R., Nasim, A., Nestmann, E.R., (1985) Recombinant DNA Methodology, pp. 190-195. , John Wiley and Sons, Inc. New York, USA
- Dodd, H.M., Bennett, P.M., (1986) J. Gen. Microbiol., 132, pp. 1009-1020
- Nudel, B.C., Texeira De Mattos, M.J., Hellingwerf, K.J., Neijssel, O.M., (1993) Cur. Microbiol., 26, pp. 281-286
- Roberts, R.C., Burioni, R., Helinski, D., (1990) J. Bacteriol., 172, pp. 6204-6216
Citas:
---------- APA ----------
Bellani, M., Nudel, C. & Sanchez Rivas, C. (1997) . Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli. Biotechnology Letters, 19(4), 331-333.http://dx.doi.org/10.1023/A:1018394616153
---------- CHICAGO ----------
Bellani, M., Nudel, C., Sanchez Rivas, C. "Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli" . Biotechnology Letters 19, no. 4 (1997) : 331-333.http://dx.doi.org/10.1023/A:1018394616153
---------- MLA ----------
Bellani, M., Nudel, C., Sanchez Rivas, C. "Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli" . Biotechnology Letters, vol. 19, no. 4, 1997, pp. 331-333.http://dx.doi.org/10.1023/A:1018394616153
---------- VANCOUVER ----------
Bellani, M., Nudel, C., Sanchez Rivas, C. Site-specific recombination system from Tn 1000(γδ) stabilises recombinant plasmids in Escherichi coli. BIOTECHNOL. LETT. 1997;19(4):331-333.http://dx.doi.org/10.1023/A:1018394616153
