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Abstract:

Tn1000 (or 'γδ') insertions in a pBR322-recombinant plasmid carrying the threonine operon (pBE) were obtained either spontaneously during chemostat cultivation (pBE-2) or selected during transconjugation (pBE-3 and pBE-4). The different insertion derivatives were tested for their stability in different host genetic backgrounds (in relation to threonine auxotrophy and recA function), various media composition (liquid or solid, rich or minimal) and growth temperatures (30°C, 37°C and 42°C). All the derivatives carrying the γδ sequence, albeit increasing their size from 9.7 to 15.6 Kb, significantly enhanced segregational and structural plasmid stability in every condition tested.

Registro:

Documento: Artículo
Título:Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli
Autor:Bellani, M.; Shlain, V.; Nudel, C.; Sanchez-Rivas, C.
Filiación:Catedra de Biotecnologia, Fac. de Farmacia y Bioquímica, UBA, Junín 956, (1113) Buenos Aires, Argentina
Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, (1428) Buenos Aires, Argentina
Palabras clave:threonine; article; auxotrophy; chemostat; escherichia coli; gene insertion sequence; operon; recombinant plasmid; temperature; Escherichia coli
Año:1997
Volumen:19
Número:1
Página de inicio:1
Página de fin:5
DOI: http://dx.doi.org/10.1023/A:1018458502718
Título revista:Biotechnology Letters
Título revista abreviado:BIOTECHNOL. LETT.
ISSN:01415492
CODEN:BILED
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01415492_v19_n1_p1_Bellani

Referencias:

  • Cossart, P., Katinka, M., Yaniv, M., Saint-Girons, I., Cohen, G.N., (1979) Molec. Gen. Genet., 175, pp. 39-44
  • Chew, L.C.K., Tacon, W.C.A., Cole, J.A., (1988) FEMS Microbiol. Lett., 56, pp. 101-104
  • Chew, L.C.K., Tacon, W.C.A., Cole, J.A., (1986) FEMS Microbiol. Lett., 36, pp. 275-280
  • Dabert, P., Ehrlich, S.D., Gruss, A., (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 12073-12077
  • Dillon, J.-A.R., Nasim, A., Nestmann, E.R., (1985) Recombinant Dna Methodology, pp. 190-195. , John Wiley and Sons, Inc. NewYork, USA
  • Dodd, H.M., Bennett, P.M., (1986) J. Gen Microbiol., 132, pp. 1009-1020
  • Guyer, M.S., (1978) J. Mol. Biol., 126, pp. 347-365
  • Kim, B.G., Shuler, M.L., (1991) Biotechnol. Bioeng., 37, pp. 1076-1086
  • Li, X.L., Robbins, J.W., Taylor, K.B., (1992) J.Ind. Microbiol., 9, pp. 1-10
  • Lopilato, J., Bortner, S., Beckwith, J., (1986) Mol. Gen. Genet., 205, pp. 285-290
  • Meacock, P., Cohen, S., (1980) Cell, 20, pp. 529-533
  • Miller, J.H., (1992) A Short Course in Bacterial Genetics, , Cold Spring Harbor Laboratory Press
  • Mizukami, T., Yagisawa, M., Oka, T., Furuya, A., (1986) Agric. Biol. Chem., 50, pp. 1019-1027
  • Nordström, K., Austin, S.J., (1989) Ann Rev. Genet., 23, pp. 37-69
  • Nudel, B.C., Corradini, C., Fraile, E.R., Giulietti, A.M., (1987) Biotechnol. Lett., 9, pp. 77-82
  • Nudel, B.C., Pueyo, M.G., Judewicz, N.D., Giulietti, A.M., (1989) Antonie van Leeuwenhoek, 56, pp. 273-282
  • Nudel, B.C., Vosman, B., De Teixeira Mattos, J., Hellingwerf, K., Neijssel, O.M., (1993) Current Microbiology, 26, pp. 281-286
  • Roberts, R.C., Burioni, R., Helinski, D.T., (1990) J. Bacteriol., 172, pp. 6204-6216-
  • Sambrook, K., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor Lab., Cold Sring Harbor, NY
  • Sayadi, S., Nasri, M., Barbotin, J.N., Thomas, D., (1989) Biotech. & Bioeng., 33, pp. 801-808
  • Sun, W.J., Lee, C., George, H.A., Powell, A.L., Dahlgren, M.E., Greasham, R., Park, C.H., (1993) Biotechnol. Lett., 15, pp. 809-814

Citas:

---------- APA ----------
Bellani, M., Shlain, V., Nudel, C. & Sanchez-Rivas, C. (1997) . Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli. Biotechnology Letters, 19(1), 1-5.
http://dx.doi.org/10.1023/A:1018458502718
---------- CHICAGO ----------
Bellani, M., Shlain, V., Nudel, C., Sanchez-Rivas, C. "Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli" . Biotechnology Letters 19, no. 1 (1997) : 1-5.
http://dx.doi.org/10.1023/A:1018458502718
---------- MLA ----------
Bellani, M., Shlain, V., Nudel, C., Sanchez-Rivas, C. "Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli" . Biotechnology Letters, vol. 19, no. 1, 1997, pp. 1-5.
http://dx.doi.org/10.1023/A:1018458502718
---------- VANCOUVER ----------
Bellani, M., Shlain, V., Nudel, C., Sanchez-Rivas, C. Tn 1000 (γδ) insertions stabilize pBR322-derived recombinant plasmids in Escherichia coli. BIOTECHNOL. LETT. 1997;19(1):1-5.
http://dx.doi.org/10.1023/A:1018458502718