Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Enzyme immobilization has attracted great interest in biotechnology processes. Herein we report the immobilization of urease from Canavalia ensiformis (jack bean) in sol-gel-derived silica nanocomposites. Urease activity, differential scanning calorimetry (DSC), nitrogen and water adsorption isotherms were used to characterize the effect of storage at various relative humidities on enzyme activity immobilized in sol-gel-derived silica nanocomposites. In this study, the nanocomposites consist of tetraethoxysilane, as inorganic silicate precursor, in combination with glycerol or trehalose as organic additives. Entrapped urease was more stable for all the formulations aged with a relative humidity of 80%. However, significant differences (p < 0.05) in enzyme activity recovered at this relative humidity were observed between samples with different formulations, reflecting the effect of additives during the immobilization process. The applications of biocompatible sol-gel-derived matrices can be further extended and utilized in the development of biosensors with immobilized biomolecules that can be used for long time periods by taking into account different factors, among which the storage relative humidity has permitted to greatly improve the stability of the immobilized urease. © 2008 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites
Autor:Desimone, M.F.; Matiacevich, S.B.; Buera, M.d.P.; Díaz, L.E.
Filiación:National Research Council (CONICET), Cátedra de Química Analítica Instrumental, Facultad de Farmacia y Bioquímica, Junín 956 Piso 3, 1113 Ciudad de Buenos Aires, Argentina
National Research Council (CONICET), Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Int. Guiraldes s/n Ciudad Universitaria, CP1428EHA Buenos Aires, Argentina
Palabras clave:Glycerol; Immobilization; Nanocomposites; Relative humidity; Sol-gel; Trehalose; Urease; Adsorption isotherms; Biotechnology; Differential scanning calorimetry; Enzyme activity; Nanocomposites; Polymers; Silica; Sol-gels; Canavalia ensiformis; Organic additives; Sol-gel-derived matrices; Atmospheric humidity; glycerol; nanocomposite; nitrogen; silicon dioxide; tetraethoxysilane; trehalose; urease; water; adsorption; article; biocompatibility; biosensor; biotechnology; Canavalia; differential scanning calorimetry; enzyme activity; enzyme immobilization; enzyme stability; gel; humidity; jack bean; nonhuman; storage; Canavalia ensiformis
Año:2008
Volumen:42
Número:7
Página de inicio:583
Página de fin:588
DOI: http://dx.doi.org/10.1016/j.enzmictec.2008.03.009
Título revista:Enzyme and Microbial Technology
Título revista abreviado:Enzyme Microb. Technol.
ISSN:01410229
CODEN:EMTED
CAS:glycerol, 56-81-5; nitrogen, 7727-37-9; silicon dioxide, 10279-57-9, 14464-46-1, 14808-60-7, 15468-32-3, 60676-86-0, 7631-86-9; trehalose, 99-20-7; urease, 9002-13-5; water, 7732-18-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01410229_v42_n7_p583_Desimone

Referencias:

  • Fágain, C.O., Enzyme stabilization-recent experimental progress (2003) Enzyme Microb Technol, 33, pp. 137-149
  • Kim, J., Grate, J.W., Wang, P., Nanostructures for enzyme stabilization (2006) Chem Eng Sci, 61, pp. 1017-1026
  • Roger, A.S., Enzyme immobilization: the quest for optimum performance (2007) Adv Synth Catal, 349, pp. 1289-1307
  • Mateo, C., Palomo, J.M., Fernandez-Lorente, G., Guisan, J.M., Fernandez-Lafuente, R., Improvement of enzyme activity, stability and selectivity via immobilization techniques (2007) Enzyme Microb Technol, 40, pp. 1451-1463
  • Gupta, R., Chaudhury, N.K., Entrapment of biomolecules in sol-gel matrix for applications in biosensors: problems and future prospects (2007) Biosens Bioelectron, 22, pp. 2387-2399
  • Campanini, B., Bologna, S., Cannone, F., Chirico, G., Mozzarelli, A., Bettati, S., Unfolding of green fluorescent protein mut2 in wet nanoporous silica gels (2005) Protein Sci, 14, pp. 1125-1133
  • Brinker, C.J., Sheerer, G.W., (1990) Sol-gel science. The physics and chemistry of sol-gel processing, , Academic Press, Inc., San Diego, CA
  • Braun, S., Rappoport, S., Zusman, R., Avnir, D., Ottolenghi, M., Biochemically active sol-gel glasses. The trapping of enzymes (1990) Mater Lett, 10, pp. 1-8
  • Ellerby, L.M., Nishida, C.R., Nishida, F., Yamanaka, S.A., Dunn, B., Valentine, J.S., Encapsulation of proteins in transparent porous silicate glasses prepared by the sol-gel method (1992) Science, 255, pp. 1113-1115
  • Alvarez, G.S., Desimone, M.F., Diaz, L.E., Immobilization of bacteria in silica matrices using citric acid in the sol-gel process (2007) Appl Microbiol Biotechnol, 73, pp. 1059-1064
  • Desimone, M.F., De Marzi, M.C., Copello, G.J., Fernandez, M.M., Malchiodi, E.L., Diaz, L.E., Efficient preservation in a silicon oxide matrix of Escherichia coli, producer of recombinant proteins (2005) Appl Microbiol Biotechnol, 68, pp. 747-752
  • Desimone, M.F., De Marzi, M.C., Copello, G.J., Fernández, M.M., Pieckenstain, F.L., Malchiodi, E.L., Production of recombinant proteins by sol-gel immobilized Escherichia coli (2006) Enzyme Microb Technol, 40, pp. 168-171
  • Livage, J., Coradin, T., Living cells in oxide glasses (2006) Rev Miner Geochem, 64, pp. 315-332
  • Avnir, D., Coradin, T., Lev, O., Livage, J., Recent bio-applications of sol-gel materials (2006) J Mater Chem, 16, pp. 1013-1030
  • Coradin, T., Boissiere, M., Livage, J., Sol-gel chemistry in medicinal science (2006) Curr Med Chem, 13, pp. 99-108
  • Coradin, T., Livage, J., Aqueous silicates in biological sol-gel applications: new perspectives for old precursors (2007) Acc Chem Res, 40, pp. 819-826
  • Kandimalla, V.B., Tripathi, V.S., Ju, H., Immobilization of biomolecules in sol-gels: biological and analytical applications (2006) Crit Rev Anal Chem, 36, pp. 73-106
  • Takahashi, H., Li, B., Sasaki, T., Miyazaki, C., Kajino, T., Inagaki, S., Immobilized enzymes in ordered mesoporous silica materials and improvement of their stability and catalytic activity in an organic solvent (2001) Microporous Mesoporous Mater, 44-45, pp. 755-762
  • Wang, P., Dai, S., Waezsada, S.D., Tsao, A.Y., Davison, B.H., Enzyme stabilization by covalent binding in nanoporous sol-gel glass for nonaqueous biocatalysis (2001) Biotechnol Bioeng, 74, pp. 249-255
  • Veith, S.R., Hughes, E., Vuataz, G., Pratsinis, S.E., Restricted diffusion in silica particles measured by pulsed field gradient NMR (2004) J Colloid Interface Sci, 274, pp. 216-228
  • Zheng, L., Reid, W.R., Brennan, J.D., Measurement of fluorescence from tryptophan to probe the environment and reaction kinetics within protein doped sol-gel derived glass monoliths (1997) Anal Chem, 69, pp. 3940-3949
  • Zheng, L., Brennan, J.D., Measurement of intrinsic fluorescence to probe the conformational flexibility and thermodynamic stability of a single tryptophan protein entrapped in a sol-gel derived glass matrix (1998) Analyst, 123, pp. 1735-1744
  • Iller, R.K., (1979) The chemistry of silica, , Wiley, New York
  • Jin, W., Brennan, J.D., Properties and applications of proteins encapsulated within sol-gel derived materials (2002) Anal Chim Acta, 461, pp. 1-36
  • Gill, I., Bio-doped nanocomposites polymers: sol-gel bioencapsulates (2001) Chem Mater, 13, pp. 3404-3421
  • Sanchez, C., Julián, B., Belleville, P., Popall, M., Applications of hybrid organic-inorganic nanocomposites (2005) J Mater Chem, 15, pp. 3559-3592
  • Coradin, T., Allouche, J., Boissière, M., Livage, J., Mimicking biogenic silica nanostructures formation (2006) Curr Nanosci, 2, pp. 219-230
  • Balamurugan, A., Kannan, S., Rajeswari, S., Synthesis of hydroxyapatite on silica gel surface by using glycerin as a drying control chemical additive (2003) Mater Lett, pp. 1244-1250
  • Patwardhan, S.V., Patwardhan, G., Perry, C.C., Interactions of biomolecules with inorganic materials: principles, applications and future prospects (2007) J Mater Chem, 17, pp. 2875-2884
  • Yiu, H.H.P., Wright, P.A., Enzymes supported on ordered mesoporous solids: a special case of an inorganic-organic hybrid (2005) J Mater Chem, 15, pp. 3690-3700
  • Sahney, R., Anand, S., Puri, B.K., Srivastava, A.K., A comparative study of immobilization techniques for urease on glass-pH-electrode and its application in urea detection in blood serum (2006) Anal Chim Acta, 578, pp. 156-161
  • Qin, Y., Cabral, J.M.S., Properties and applications of urease (2002) Biocatal Biotransfor, 20, pp. 1-14
  • Kuralay, F., Özyörük, H., Yildiz, A., Inhibitive determination of Hg2+ ion by an amperometric urea biosensor using poly(vinylferrocenium) film (2007) Enzyme Microb Technol, 40, pp. 1156-1159
  • Rodriguez, B.B., Bolbot, J.A., Tothill, I.E., Development of urease and glutamic dehydrogenase amperometric assay for heavy metals screening in polluted samples (2004) Biosens Bioelectron, 19, pp. 1157-1167
  • Miyagawa, K., Sumida, M., Nakao, M., Harada, M., Yamamoto, H., Kusumi, T., Purification, characterization, and application of an acid urease from Arthrobacter mobilis (1999) J Biotechnol, 68, pp. 227-236
  • Cullen, D.C., Sethi, R.S., Lowe, C.R., Multi-analyte miniature conductance biosensor (1990) Anal Chim Acta, 231, pp. 33-40
  • Alonso, A., Almendral, M.J., Baez, M.D., Porras, M.J., Alonso, C., Enzyme immobilization on an epoxy matrix. Determination of l-arginine by flow-injection techniques (1995) Anal Chim Acta, 308, pp. 164-169
  • Sansubrino, A., Mascini, M., Development of an optical fibre sensor for ammonia, urea, urease and IgG (1994) Biosens Bioelectron, 9, pp. 207-216
  • Ercole, C., Gallo, M.D., Mosiello, L., Baccella, S., Lepidi, A., Escherichia coli detection in vegetable food by a potentiometric biosensor (2003) Sens Actuators B-Chem, 91, pp. 163-168
  • Tsai, H.C., Doong, R.A., Preparation and characterization of urease-encapsulated biosensors in poly(vinyl alcohol)-modified silica sol-gel materials (2007) Biosens Bioelectron, 23, pp. 66-73
  • Desimone, M.F., Degrossi, J., D'Aquino, M., Diaz, L.E., Ethanol tolerance in free and sol-gel immobilized Saccharomyces cerevisiae (2002) Biotechnol Lett, 24, pp. 1557-1559
  • Desimone, M.F., Degrossi, J., D'Aquino, M., Diaz, L.E., Sol-gel immobilisation of Saccharomyces cerevisiae enhances viability in organic media (2003) Biotechnol Lett, 25, pp. 671-674
  • Greenspan, L., National bureau of standards (1977) J Res, 81 (A), p. 1
  • Witte, C.P., Escobar, N.M., In-gel detection of urease with nitroblue tetrazolium and quantification of the enzyme from different crop plants using the indophenol reaction (2001) Anal Biochem, 290, pp. 102-106
  • Lomauro, C.J., Bakshi, A.S., Labuza, T.P., The evaluation of G.A.B. constants from water vapor sorption data (1985) Lebensmittel-Wissenschaft Technol, 18, pp. 225-229
  • Gregg, S.J., Sing, K.S.W., (1982) Adsorption surface area and porosity. 2nd ed., , Academic Press, New York
  • Ferrer, M.L., Carbajal, Z.Y., Yuste, L., Rojo, F., del Monte, F., Bacteria viability in sol-gel materials revisited: cryo-SEM as a suitable tool to study the structural integrity of encapsulated bacteria (2006) Chem Mater, 18, pp. 1458-1463
  • Gordon, M., Taylor, J.S., Ideal copolymers and the second order transitions of synthetic rubbers. I: Noncrystalline copolymers (1952) J Appl Chem, 2, pp. 493-500
  • Katkov, I.I., Levine, F., Prediction of the glass transition temperature of water solutions: comparison of different models (2004) Cryobiology, 49, pp. 62-82
  • Robens, E., Dabrowski, A., Kutarov, V.V., Gravimetric volumetric and calorimetric studies of the surface structure of Portland cement (2000) J Therm Anal Calorim, 62, pp. 435-441
  • Buera, P., Schebor, C., Elizalde, B., Effects of carbohydrate crystallization on stability of dehydrated foods and ingredient formulations (2005) J Food Eng, 67, pp. 157-165
  • Roos, Y.H., Haque, M.K., Water plasticization and crystallization of lactose in spray-dried lactose/protein mixtures (2004) J Food Sci, 69, pp. 23-29
  • Mazzobre, F., Longinotti, P., Corti, H., Buera, P., Effect of salts on the properties of aqueous sugar systems, in relation to biomaterial stabilization. 1: Water sorption behavior and ice crystallization/meeting (2001) Cryobiology, 43, pp. 199-210
  • Gonnelli, M., Strambini, G.B., Structure and dynamics of proteins encapsulated in silica hydrogels by Trp phosphorescence (2003) Biophys Chem, 104, pp. 155-169
  • Keeling-Tucker, T., Brennan, J.D., Fluorescent probes as reporters on the local structure and dynamics in sol-gel-derived nanocomposite materials (2001) Chem Mater, 13, pp. 3331-3350
  • DePaz, R.A., Dale, D.A., Barnett, C.C., Carpenter, J.F., Gaertner, A.L., Randolph, T.W., Effects of drying methods and additives on the structure, function, and storage stability of subtilisin: role of protein conformation and molecular mobility (2002) Enzyme Microb Technol, 31, pp. 765-774
  • Benz, D.P., Garboczi, E.J., Quenard, D.A., Modelling drying shrinkage in reconstructed porous materials (1998) Mater Sci Eng, 6, pp. 211-236
  • Gadre, S.Y., Gouma, P.I., Biodoped ceramics: synthesis, properties, and applications (2006) J Am Ceram Soc, 89, pp. 2987-3002
  • Goettmann, F., Sanchez, C., How does confinement affect the catalytic activity of mesoporous materials? (2007) J Mater Chem, 17, pp. 24-30

Citas:

---------- APA ----------
Desimone, M.F., Matiacevich, S.B., Buera, M.d.P. & Díaz, L.E. (2008) . Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites. Enzyme and Microbial Technology, 42(7), 583-588.
http://dx.doi.org/10.1016/j.enzmictec.2008.03.009
---------- CHICAGO ----------
Desimone, M.F., Matiacevich, S.B., Buera, M.d.P., Díaz, L.E. "Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites" . Enzyme and Microbial Technology 42, no. 7 (2008) : 583-588.
http://dx.doi.org/10.1016/j.enzmictec.2008.03.009
---------- MLA ----------
Desimone, M.F., Matiacevich, S.B., Buera, M.d.P., Díaz, L.E. "Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites" . Enzyme and Microbial Technology, vol. 42, no. 7, 2008, pp. 583-588.
http://dx.doi.org/10.1016/j.enzmictec.2008.03.009
---------- VANCOUVER ----------
Desimone, M.F., Matiacevich, S.B., Buera, M.d.P., Díaz, L.E. Effects of relative humidity on enzyme activity immobilized in sol-gel-derived silica nanocomposites. Enzyme Microb. Technol. 2008;42(7):583-588.
http://dx.doi.org/10.1016/j.enzmictec.2008.03.009