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Abstract:

We report the efficient enantioselective reduction of a number of β-ketoesters by mycelial and yeast-like forms of the dimorphic fungus Mucor rouxii in a whole-cell process. Mycelial cells, grown in aerobiosis, were efficient in water, whereas the yeast-like cells, grown in anaerobic medium, were both efficient in water and in organic solvents. Almost 100% of conversion with 97% of enantiomeric excess of the (S) forms of the reduced β-ketoesters was obtained at 15 g biomass (wet weight)/mmol substrate in two hours. The fungal spores, which are the physiologically resistant form of the fungus, also catalyzed the reductive process efficiently and stereoselectively. The freeze-dried as well as the warm-air dried yeast-like cells, rehydrated in a small volume of water, maintained the same efficiency and selectivity of the reaction in organic solvents as the fresh biomass up to at least 4 months. © 2002 Elsevier Science Inc. All rights reserved.

Registro:

Documento: Artículo
Título:Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii
Autor:Mangone, C.P.; N.Pereyra, E.; Argimón, S.; Moreno, S.; Baldessari, A.
Filiación:Departamento De Química Orgánica, Facultad De Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Departamento De Química Biológica, Facultad De Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina
Palabras clave:β-Ketoesters; Mucor rouxii; Mycelial; Spores; Yeast; Biomass; Cells; Esters; Fungi; Organic solvents; Water; Yeast; Aerobiosis; Stereoselectivity; Reduction; ester derivative; organic solvent; water; aerobic metabolism; article; bacterial spore; biomass; catalysis; controlled study; enantiomer; enantioselectivity; fungal cell; fungus; fungus growth; fungus spore; morphology; Mucor; mucor rouxii; mycelium; nonhuman; physiology; reduction; rehydration; stereochemistry; yeast; Amylomyces rouxii; Fungi; Mucor
Año:2002
Volumen:30
Número:5
Página de inicio:596
Página de fin:601
DOI: http://dx.doi.org/10.1016/S0141-0229(01)00516-6
Título revista:Enzyme and Microbial Technology
Título revista abreviado:Enzyme Microb. Technol.
ISSN:01410229
CODEN:EMTED
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01410229_v30_n5_p596_Mangone

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Citas:

---------- APA ----------
Mangone, C.P., N.Pereyra, E., Argimón, S., Moreno, S. & Baldessari, A. (2002) . Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii. Enzyme and Microbial Technology, 30(5), 596-601.
http://dx.doi.org/10.1016/S0141-0229(01)00516-6
---------- CHICAGO ----------
Mangone, C.P., N.Pereyra, E., Argimón, S., Moreno, S., Baldessari, A. "Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii" . Enzyme and Microbial Technology 30, no. 5 (2002) : 596-601.
http://dx.doi.org/10.1016/S0141-0229(01)00516-6
---------- MLA ----------
Mangone, C.P., N.Pereyra, E., Argimón, S., Moreno, S., Baldessari, A. "Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii" . Enzyme and Microbial Technology, vol. 30, no. 5, 2002, pp. 596-601.
http://dx.doi.org/10.1016/S0141-0229(01)00516-6
---------- VANCOUVER ----------
Mangone, C.P., N.Pereyra, E., Argimón, S., Moreno, S., Baldessari, A. Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii. Enzyme Microb. Technol. 2002;30(5):596-601.
http://dx.doi.org/10.1016/S0141-0229(01)00516-6