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Abstract:

The Doehlert design was applied to optimize water activity, pH, and fermentation time conditions for Aspergillus niger 148 pectinolytic activities production in solid-state (SSF) and submerged (SmF) fermentation. The fermentation technique had a great influence on the composition of pectinases produced by the fungus. Production of polygalacturonase was 5-fold higher in SmF than in SSF. However, pectin lyase production was 3 times higher in SSF than in SmF. Pectinesterase was only a 30% higher in SSF than in SmF. Optimization of the process was based on minimum pectinesterase production and maximal pectin lyase production. The optimal conditions to obtain the aforecited composition by a SSF process were initial pH 6.5-7, initial a(w) 0.93-0.94, and 2 days of fermentation. For the SmF process they were initial pH 5.5-6.2, initial a(w) above 0.99, and 3 days of fermentation. The results suggest that A. niger 148 overproduces pectin lyase under the optimized SSF culture conditions, as the concentration in the fermentation extract was around 0.25 g/l, representing ~65% of total extracellular proteins produced by the fungus. Copyright (C) 1999 Elsevier Science Inc. The Doehlert design was applied to optimize water activity, pH, and fermentation time conditions for Aspergillus niger 148 pectinolytic activities production in solid-state (SSF) and submerged (SmF) fermentation. The fermentation technique had a great influence on the composition of pectinases produced by the fungus. Production of polygalacturonase was 5-fold higher in SmF than in SSF. However, pectin lyase production was 3 times higher in SSF than in SmF. Pectinesterase was only a 30% higher in SSF than in SmF. Optimization of the process was based on minimum pectinesterase production and maximal pectin lyase production. The optimal conditions to obtain the aforecited composition by a SSF process were initial pH 6.5-7, initial aw 0.93-0.94, and 2 days of fermentation. For the SmF process they were initial pH 5.5-6.2, initial aw above 0.99, and 3 days of fermentation. The results suggest that A. niger 148 overproduces pectin lyase under the optimized SSF culture conditions, as the concentration in the fermentation extract was around 0.25 g/l, representing ≈65% of total extracellular proteins produced by the fungus.

Registro:

Documento: Artículo
Título:Application of Doehlert designs for water activity, pH, and fermentation time optimization for Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation
Autor:Taragano, V.M.; Pilosof, A.M.R.
Ciudad:New York, NY, United States
Filiación:Lab. de Tecn. de Alimentos, Depto. Industrias, Fac. Cie. E., 1428 Buenos Aires, Argentina
Palabras clave:Aspergillus niger; Pectinases; Response surface methodology; Solid-state fermentation; Submerged fermentation; Composition; Enzymes; Fermentation; Fungi; Optimization; pH; Proteins; Temperature; Water; Aspergillus niger; Doehlert design; Pectin lyase; Pectinases; Polygalacturonase; Response surface methodology; Solid state fermentation; Submerged fermentation; Water activity; Microorganisms; polygalacturonase; article; aspergillus niger; cell suspension; enzyme activity; fermentation; model; pH; technique; theory; Aspergillus niger
Año:1999
Volumen:25
Número:3-5
Página de inicio:411
Página de fin:419
DOI: http://dx.doi.org/10.1016/S0141-0229(99)00065-4
Título revista:Enzyme and Microbial Technology
Título revista abreviado:Enzyme Microb. Technol.
ISSN:01410229
CODEN:EMTED
CAS:polygalacturonase, 9023-92-1, 9032-75-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01410229_v25_n3-5_p411_Taragano

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Citas:

---------- APA ----------
Taragano, V.M. & Pilosof, A.M.R. (1999) . Application of Doehlert designs for water activity, pH, and fermentation time optimization for Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation. Enzyme and Microbial Technology, 25(3-5), 411-419.
http://dx.doi.org/10.1016/S0141-0229(99)00065-4
---------- CHICAGO ----------
Taragano, V.M., Pilosof, A.M.R. "Application of Doehlert designs for water activity, pH, and fermentation time optimization for Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation" . Enzyme and Microbial Technology 25, no. 3-5 (1999) : 411-419.
http://dx.doi.org/10.1016/S0141-0229(99)00065-4
---------- MLA ----------
Taragano, V.M., Pilosof, A.M.R. "Application of Doehlert designs for water activity, pH, and fermentation time optimization for Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation" . Enzyme and Microbial Technology, vol. 25, no. 3-5, 1999, pp. 411-419.
http://dx.doi.org/10.1016/S0141-0229(99)00065-4
---------- VANCOUVER ----------
Taragano, V.M., Pilosof, A.M.R. Application of Doehlert designs for water activity, pH, and fermentation time optimization for Aspergillus niger pectinolytic activities production in solid-state and submerged fermentation. Enzyme Microb. Technol. 1999;25(3-5):411-419.
http://dx.doi.org/10.1016/S0141-0229(99)00065-4