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Abstract:

INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) encodes a putative DEVH-box RNA helicase originally identified through a genetic screening for Arabidopsis mutants altered in plasmodesmata (PD) aperture. Depletion of ISE2 also affects chloroplasts activity, decreases accumulation of photosynthetic pigments and alters expression of photosynthetic genes. In this work, we show the chloroplast localization of ISE2 and decipher its role in plastidic RNA processing and, consequently, PD function. Group II intron-containing RNAs from chloroplasts exhibit defective splicing in ise2 mutants and ISE2-silenced plants, compromising plastid viability. Furthermore, RNA immunoprecipitation suggests that ISE2 binds in vivo to several splicing-regulated RNAs. Finally, we show that the chloroplast clpr2 mutant (defective in a subunit of a plastidic Clp protease) also exhibits abnormal PD function during embryogenesis, supporting the idea that chloroplast RNA processing is required to regulate cell-cell communication in plants. © 2016 John Wiley & Sons Ltd.

Registro:

Documento: Artículo
Título:The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing
Autor:Carlotto, N.; Wirth, S.; Furman, N.; Ferreyra Solari, N.; Ariel, F.; Crespi, M.; Kobayashi, K.
Filiación:Laboratorio de Agrobiotecnologia, Instituto de Biodiversidad y Biologia Experimental Aplicada, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA), CONICET - Partner Institute of the Max Planck Society, Buenos Aires, Argentina
Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, Orsay, 91405, France
Palabras clave:Cell-to-cell communication; Chloroplast; Retrograde signals; cells and cell components; chloroplast; dicotyledon; enzyme activity; gene expression; genetic analysis; photosynthesis; pigment; RNA; Arabidopsis; Arabidopsis protein; chloroplast RNA; DEAD box protein; INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis; RNA helicase; Arabidopsis; chloroplast; drug effects; enzymology; gene expression regulation; genetics; intron; metabolism; mutation; photosynthesis; plasmodesma; reporter gene; RNA splicing; seedling; transgenic plant; transport at the cellular level; Arabidopsis; Arabidopsis Proteins; Biological Transport; Chloroplasts; DEAD-box RNA Helicases; Gene Expression Regulation, Plant; Genes, Reporter; Introns; Mutation; Photosynthesis; Plants, Genetically Modified; Plasmodesmata; RNA Helicases; RNA Splicing; RNA, Chloroplast; Seedlings
Año:2016
Volumen:39
Número:1
Página de inicio:165
Página de fin:173
DOI: http://dx.doi.org/10.1111/pce.12603
Título revista:Plant Cell and Environment
Título revista abreviado:Plant Cell Environ.
ISSN:01407791
CODEN:PLCED
CAS:interferon induced helicase C domain containing protein 1; RNA helicase; Arabidopsis Proteins; DEAD-box RNA Helicases; INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis; RNA Helicases; RNA, Chloroplast
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01407791_v39_n1_p165_Carlotto

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Citas:

---------- APA ----------
Carlotto, N., Wirth, S., Furman, N., Ferreyra Solari, N., Ariel, F., Crespi, M. & Kobayashi, K. (2016) . The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing. Plant Cell and Environment, 39(1), 165-173.
http://dx.doi.org/10.1111/pce.12603
---------- CHICAGO ----------
Carlotto, N., Wirth, S., Furman, N., Ferreyra Solari, N., Ariel, F., Crespi, M., et al. "The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing" . Plant Cell and Environment 39, no. 1 (2016) : 165-173.
http://dx.doi.org/10.1111/pce.12603
---------- MLA ----------
Carlotto, N., Wirth, S., Furman, N., Ferreyra Solari, N., Ariel, F., Crespi, M., et al. "The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing" . Plant Cell and Environment, vol. 39, no. 1, 2016, pp. 165-173.
http://dx.doi.org/10.1111/pce.12603
---------- VANCOUVER ----------
Carlotto, N., Wirth, S., Furman, N., Ferreyra Solari, N., Ariel, F., Crespi, M., et al. The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing. Plant Cell Environ. 2016;39(1):165-173.
http://dx.doi.org/10.1111/pce.12603