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Abstract:

The white rot fungus Trametes trogii strain BAFC 463 produced laccase, manganese peroxidase, lignin peroxidase and cellobiose dehydrogenase, as well as two hydrogen peroxide-producing activities: glucose oxidizing activity and glyoxal oxidase. In high-N (40 mM N) cultures, the titres of laccase, MnP and GLOX were 27 (6.55 U/ml), 45 (403.00 mU/ml) and 8 (32,14 mU/ml) fold higher, respectively, than those measured in an N-limited medium. This is consistent with the fact that the ligninolytic system of T. trogii is expressed constitutively. Lower activities of all the enzymes tested were recorded upon decreasing the initial pH of the medium from 6.5 to 4.5. Adding veratryl alcohol improved GLOX production, while laccase activity was stimulated by tryptophan. Supplying Tween 80 strongly reduced the activity of both MnP and GLOX, but increased laccase production. The titre of MnP was affected by the concentration of Mn in the culture medium, the highest levels were obtained with 90 μM Mn (II). LiP activity, as CDH activity, were detected only in the medium supplemented with sawdust. In this medium, laccase production reached a maximum of 4.75 U/ml, MnP 747.60 mU/ml and GLOX 117.11 mU/ml. LiP, MnP and GLOX activities were co-induced, attaining their highest levels at the beginning of secondary metabolism, but while MnP, laccase, GLOX and CDH activities were also present in the primary growth phase, LiP activity appears to be idiophasic. The simultaneous presence of high ligninolytic and hydrogen peroxide producing activities in this fungus makes it an attractive microorganism for future biotechnological applications.

Registro:

Documento: Artículo
Título:Ligninolytic enzymes of the white rot basidiomycete Trametes trogii
Autor:Levin, L.; Forchiassin, F.
Filiación:Universidad de Buenos Aires, Fac. de Ciencias Exactas y Naturales, Depto. de Ciencias Biológicas, 1428 Buenos Aires, Argentina
Palabras clave:fungal enzyme; glucose; hydrogen peroxide; laccase; lignin; lignin peroxidase; manganese peroxidase; polysorbate 80; tryptophan; veratryl alcohol; article; Basidiomycetes; biotechnology; controlled study; culture medium; enzyme activity; enzyme metabolism; enzyme synthesis; fungus culture; fungus growth; nonhuman; pH; supplementation; Trametes
Año:2001
Volumen:21
Número:2
Página de inicio:179
Página de fin:186
DOI: http://dx.doi.org/10.1002/1521-3846(200105)21:2<179::AID-ABIO179>3.0.CO;2-2
Título revista:Acta Biotechnologica
Título revista abreviado:Acta Biotechnol.
ISSN:01384988
CODEN:ACBTD
CAS:glucose, 50-99-7, 84778-64-3; hydrogen peroxide, 7722-84-1; laccase, 80498-15-3; lignin peroxidase, 42613-30-9; lignin, 9005-53-2; manganese peroxidase, 114995-15-2; polysorbate 80, 8050-83-7, 9005-65-6; tryptophan, 6912-86-3, 73-22-3; veratryl alcohol, 93-03-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01384988_v21_n2_p179_Levin

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Citas:

---------- APA ----------
Levin, L. & Forchiassin, F. (2001) . Ligninolytic enzymes of the white rot basidiomycete Trametes trogii. Acta Biotechnologica, 21(2), 179-186.
http://dx.doi.org/10.1002/1521-3846(200105)21:2<179::AID-ABIO179>3.0.CO;2-2
---------- CHICAGO ----------
Levin, L., Forchiassin, F. "Ligninolytic enzymes of the white rot basidiomycete Trametes trogii" . Acta Biotechnologica 21, no. 2 (2001) : 179-186.
http://dx.doi.org/10.1002/1521-3846(200105)21:2<179::AID-ABIO179>3.0.CO;2-2
---------- MLA ----------
Levin, L., Forchiassin, F. "Ligninolytic enzymes of the white rot basidiomycete Trametes trogii" . Acta Biotechnologica, vol. 21, no. 2, 2001, pp. 179-186.
http://dx.doi.org/10.1002/1521-3846(200105)21:2<179::AID-ABIO179>3.0.CO;2-2
---------- VANCOUVER ----------
Levin, L., Forchiassin, F. Ligninolytic enzymes of the white rot basidiomycete Trametes trogii. Acta Biotechnol. 2001;21(2):179-186.
http://dx.doi.org/10.1002/1521-3846(200105)21:2<179::AID-ABIO179>3.0.CO;2-2