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Abstract:

Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The analysis of the nucleotide sequences permitted the evaluation of the molecular modifications experienced during the evolutionary process in primates.

Registro:

Documento: Artículo
Título:A new world monkey microsatellite (AP74) higly conserved in primates
Autor:Oklander, L.I.; Steinberg, E.R.; Mudry, M.D.
Filiación:IBS (Instituto de Biología Subtropical), Facultad de Ciencias Forestales, Universidad Nacional de Misiones, Puerto Iguazú, Misiones, Argentina
Servicio de Huellas Digitales Genéticas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina
Grupo de Investigación en Biología Evolutiva, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
CONICET, Argentina
Palabras clave:Genetic variability; Genomic conservation; Microsatellite markers; Non-invasive sampling; PCR amplification; Primates; Alouatta; Alouatta caraya; Ateles belzebuth; Ateles belzebuth chamek; Ateles sp.; Haplorhini; Homo sapiens; Lemur; Lemur catta; Lemuridae; Pan troglodytes; Papio; Papio sp.; Platyrrhini; Primates
Año:2012
Volumen:17
Número:1
Página de inicio:93
Página de fin:101
Título revista:Acta Biologica Colombiana
Título revista abreviado:Acta Biol. Colomb.
ISSN:0120548X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0120548X_v17_n1_p93_Oklander

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Citas:

---------- APA ----------
Oklander, L.I., Steinberg, E.R. & Mudry, M.D. (2012) . A new world monkey microsatellite (AP74) higly conserved in primates . Acta Biologica Colombiana, 17(1), 93-101.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0120548X_v17_n1_p93_Oklander [ ]
---------- CHICAGO ----------
Oklander, L.I., Steinberg, E.R., Mudry, M.D. "A new world monkey microsatellite (AP74) higly conserved in primates " . Acta Biologica Colombiana 17, no. 1 (2012) : 93-101.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0120548X_v17_n1_p93_Oklander [ ]
---------- MLA ----------
Oklander, L.I., Steinberg, E.R., Mudry, M.D. "A new world monkey microsatellite (AP74) higly conserved in primates " . Acta Biologica Colombiana, vol. 17, no. 1, 2012, pp. 93-101.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0120548X_v17_n1_p93_Oklander [ ]
---------- VANCOUVER ----------
Oklander, L.I., Steinberg, E.R., Mudry, M.D. A new world monkey microsatellite (AP74) higly conserved in primates . Acta Biol. Colomb. 2012;17(1):93-101.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0120548X_v17_n1_p93_Oklander [ ]