Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The golden (Syrian) hamster is a seasonal breeder, and exposure of adult animals to short days results in severe gonadal regression with morphological features that resemble the immature testis. The purpose of this study was to investigate testicular steroidogenic capacity in the golden hamster and to analyse the influence of age and photoperiod on this process. Hamsters aged 36 days were maintained on a long photoperiod (14L:10D), and adult animals were then exposed to a long or a short photoperiod (6L:18D) for 14 weeks (the period of time required to achieve maximal gonadal regression), to assess circulating levels and in vitro production of testosterone, dihydrotestosterone and androstane-3α,17β-diol. In peripubertal hamsters, androstane-3α,17β-diol was the main circulating androgen detected, whereas in active adult animals, testosterone showed the highest serum levels. In hamsters exposed to a short photoperiod, blood testosterone levels were significantly lower than levels in adult hamsters exposed to a long photoperiod. Exposure of adult hamsters to a short photoperiod produced a marked reduction in serum concentrations of dihydrotestosterone and androstane-3α,17β-diol, which was not accompanied by a decrease in testicular 5α-reductase activity. In the in vitro experiments, active adult testes were less sensitive than inactive adult testes to stimulation of androgen production with hCG, but showed similar sensitivity to the gonads from hamsters aged 36 days. In accordance with circulating androgen concentrations, the principal androgens produced in the in vitro assays from peripubertal and normal adult testes were androstane-3α,17β-diol and testosterone, respectively. Unexpectedly, the main androgen produced from regressed testes under in vitro conditions was androstane-3α,17β-diol. Inactive gonads released more androstane-3α,17β-diol than did normal adult testes and total in vitro androgen production (testosterone + dihydrotestosterone + androstane-3α,17β-diol) from adult testes was not diminished by exposure to a short photoperiod. However, in spite of the significant increase detected in production of androstane-3α, 17β-diol in vitro from regressed testes, inactive gonads produced less androstane-3α,17β-diol than did peripubertal testes. In summary, our studies suggest that testicular androgen biosynthetic capacity in adult hamsters exposed to short photoperiod is not reduced and these regressed testes represent an intermediate physiological state between peripubertal and active adult testes. The significant decrease detected in serum androgen concentrations during the involution phase could result from the absence of stimulating pituitary factors, together with a negative regulation of steroidogenesis by different non-steroidal signals originating within and/or outside of the testis.

Registro:

Documento: Artículo
Título:Influence of age and photoperiod on steroidogenic function of the testis in the golden hamster
Autor:Frungieri, M.B.; Gonzalez-Calvar, S.I.; Bartke, A.; Calandra, R.S.
Filiación:Inst. de Biología y Med. Exp., Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Facultad de Medicina, UBA, Paraguay 2155, 1121 Buenos Aires, Argentina
Department of Physiology, School of Medicine, Southern Illinois University, Carbondale, IL 62901, United States
Catedra de Endocrinologia, Facultad de Ciencias Exactas, UNLP, 47 y 115, 1900 La Plata, Argentina
Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:3α-diol; 5α-reductase; Dihydrotestosterone; Golden hamster; Leydig cells; Photoperiod; Testis; Testosterone; androgen; androstanediol; androstanolone; chorionic gonadotropin; steroid 5alpha reductase; testosterone; age; androgen synthesis; animal experiment; animal tissue; article; controlled study; hamster; hormone blood level; Leydig cell; male; nonhuman; photoperiodicity; priority journal; puberty; steroidogenesis; testis; testosterone blood level; Aging; Androgens; Androstane-3,17-diol; Animals; Body Weight; Chorionic Gonadotropin; Cricetinae; Dihydrotestosterone; Male; Mesocricetus; Organ Size; Photoperiod; Sexual Maturation; Steroids; Testis; Testosterone; Testosterone 5-alpha-Reductase
Año:1999
Volumen:22
Número:4
Página de inicio:243
Página de fin:252
DOI: http://dx.doi.org/10.1046/j.1365-2605.1999.00175.x
Título revista:International Journal of Andrology
Título revista abreviado:Int. J. Androl.
ISSN:01056263
CODEN:IJAND
CAS:Androgens; Androstane-3,17-diol, 25126-76-5; Chorionic Gonadotropin; Dihydrotestosterone, 521-18-6; Steroids; Testosterone 5-alpha-Reductase, EC 1.3.99.5; Testosterone, 58-22-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01056263_v22_n4_p243_Frungieri

Referencias:

  • Bartke, A., Male hamster reproductive endocrinology (1985) The Hamster, pp. 73-98. , Ed. H. I. Spiegel. Plenum Press, New York
  • Bartke, A., Matt, K.S., Amador, A.G., Klemcke, H.G., Brown, D., Gonzales, D., Hogan, M.P., Effects of inhibitory and stimulatory photoperiods and sexual maturation on the ability of hamster testes to respond to hCG in vitro (1985) International Journal of Andrology, 8, pp. 232-242
  • Bartke, A., Sinha Hikim, A.P., Russell, L.D., Leydig cell structure and function in seasonal breeders (1996) The Leydig Cell, pp. 431-450. , eds. A.H. Payne, M.P. Hardy and L.D. Russell. Cache River Press, Vienna, IL, USA
  • Berndtson, W.E., Desjardins, C., Circulating LH and FSH levels and testicular function in hamsters during light deprivation and subsequent photoperiodic stimulation (1974) Endocrinoloyy, 95, pp. 195-205
  • Bex, F.J., Bartke, A., Testicular LH binding in the hamster: Modification by photoperiod and prolactin (1977) Endocrinology, 100, pp. 1223-1226
  • Bex, F.J., Bartke, A., Goldman, B.D., Dalterio, S., Prolactin, growth hormone, luteinizing hormone receptors, and seasonal changes in testicular activity in the golden hamster (1978) Endocrinology, 103, pp. 2069-2080
  • Bortolussi, M., Zanchetta, R., Belvedere, P., Colombo, L., Sertoli and Leydig cell numbers and gonadotropin receptors in rat testis from birth to puberty (1990) Cell and Tissue Research, 260, pp. 185-191
  • Chandrashekar, V., Bartke, A., The influence of short photoperiod on testicular and circulating levels of Testosterone precursors in the adult Golden hamster (1989) Biology of Reproduction, 40, pp. 300-306
  • Darrow, J.M., Davis, F.C., Elliot, J.A., Stetson, M.H., Turek, F.W., Menaker, M., Influence of photoperiod on reproductive development in the Golden hamster (1980) Biology of Reproduction, 22, pp. 443-450
  • Ellis, L.C., Inhibition of rat testicular androgen synthesis in vitro by melatonin and serotonin (1972) Endocrinology, 90, pp. 17-28
  • Frungieri, M.B., Gonzalez-Calvar, S.I., Calandra, R.S., Polyamine levels in testes and seminal vesicles from adult golden hamsters during gonadal regression-recrudescence (1996) Journal of Andrology, 17, pp. 683-691
  • Frungieri, M.B., Gonzalez-Calvar, S.I., Calandra, R.S., Influence of photoinhibition on GABA and glutamic acid levels, and on glutamate decarboxylase activity in the testis and epididymis of the golden hamster (1996) International Journal of Andrology, 19, pp. 171-178
  • Frungieri, M.B., Gonzalez-Calvar, S.I., Chandrashekar, V., Rao, J.N., Bartke, A., Calandra, R.S., Testicular gamma-aminobutyric acid and circulating androgens in Syrian and Djungarian hamsters during sexual development (1996) International Journal of Andrology, 19, pp. 164-170
  • Gaston, S., Menaker, M., Photoperiodic control of hamster testis (1967) Science, 167, pp. 925-928
  • Ge, R.-S., Shan, L.-X., Hardy, M.P., Pubertal development of Leydig cells (1996) The Leydig Cell, pp. 159-174. , eds. A. H. Payne, M. P. Hardy and L. D. Russell. Cache River Press, Vienna, IL, USA
  • Giusti, M., Valenti, S., De Rose, A.F., Foppiani, L., Carmignani, G., Giordano, G., Melatonin exerts a modulatory role on androgen secretion by Leydig cells: Preliminary data on in vitro cultured Leydig cells of human testis (1997) International Journal of Andrology, 20, pp. 7-12
  • Gonzalez, S.I., Cigorraga, S.B., Suescun, M.O., Calandra, R.S., Prolactin effects on testicular interstitial cells in primary cultures (1990) Acta Physiologica, Pharmacologica et Therapeutica Latino-americana, 40, pp. 65-79
  • Hall, P.F., Testicular steroid synthesis: Organization and regulation (1994) The Physiology of Reproduction, 1, pp. 1335-1362. , eds E. Knobil & J. D. Neill, Raven Press, New York
  • Heindel, J.J., Age-related and testicular regression-induced changes in adenosine 3′,5′-monophosphate responses in cultured hamster Sertoli cells (1988) Endocrinology, 122, pp. 475-481
  • Jolinson, L., Matt, K.S., Bartke, A., Nguyen, H.B., Le, H.T., Effect of photoperiod on the size of the Leydig cells in adult Syrian hamsters (1987) Biology of Reproduction, 37, pp. 727-738
  • Klemcke, H., Bartke, A., Borer, K.T., Hogan, M.P., Regulation of testicular prolactin and luteinizing hormone receptors in golden hamster (1984) Endocrinology, 114, pp. 594-603
  • Klemcke, H.G., Bartke, A., Goldman, B.D., Plasma prolactin concentrations and testicular human gonadotropin binding sites during short photoperiod-induced testicular regression and recrudescence in the golden hamster (1981) Biology of Reproduction, 25, pp. 536-548
  • Knotts, L.K., Glass, J.D., Effects of photoperiod, betaendorphin, and naloxone on in vitro secretion of testosterone in white-footed mouse (Peromycus leucopus) testes (1988) Biology of Reproduction, 39, pp. 205-212
  • Lerchl, A., Nieschlag, E., Diurnal variations of serum and testicular testosterone and dihydrotestosterone (DHT) in Djungarian hamsters (Phodopus sungorus): Testes are the main source for circulating DHT (1995) General and Comparative Endocrinology, 98, pp. 129-136
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) Journal of Biological Chemistry, 193, pp. 265-275
  • Mayerhofer, A., Bartke, A., Steger, R.W., Catecholamine effects on testicular testosterone production in the gonadally active and the gonadally regressed adult golden hamster (1989) Biology of Reproduction, 40, pp. 752-761
  • Niedziela, M., Lukaszyk, A., Melatonin inhibits forskolin stimulated testosterone secretion by hamster Leydig cells in primary culture (1993) Melatonin and the Pineal Gland: From Basic Science to Clinical Application, pp. 285-288. , eds J. Touitou, J. Arendt & P. Pevet. Elsevier, Amsterdam
  • Ojeda, S.R., Urbanski, H.F., Puberty in the rat (1994) The Physiology of Reproduction, 2, pp. 363-409. , eds E. Knobil & J. D. Neill, Raven Press, New York
  • Reiter, R.J., The pineal and its hormones in the control of reproduction in mammals (1980) Endocrine Reviews, 1, pp. 109-131
  • Shan, L.-X., Hardy, M.P., Developmental changes in levels of luteinizing hormone receptor and androgen receptor in rat Leydig cells (1992) Endocrinology, 131, pp. 1107-1114
  • Sinha Hikim, A.P., Amador, A.G., Bartke, A., Russell, L.D., Structure/function relationships in active and inactive hamster Leydig cells: A correlative morphometric and endocrine study (1989) Endocrinology, 125, pp. 1844-1856
  • Sinha Hikim, A.P., Bartke, A., Russell, D., The seasonal breeding hamster as a model to study structure-function relationships in the testis (1988) Tissue and Cell, 20, pp. 63-78
  • Sinha Hikim, A.P., Bartke, A., Russell, D., Morphometric studies on hamster testes in gonadally active and inactive states: Light microscope findings (1988) Biology of Reproduction, 39, pp. 1225-1237
  • Sinha Hikim, A.P., Chandrashekar, V., Bartke, A., Russell, L.D., Sentinels of Leydig cell structural and functional changes in golden hamsters in early testicular regression and recrudescence (1993) International Journal of Andrology, 16, pp. 324-342
  • Stocco, D.M., Acute regulation of Leydig cell steroidogenesis (1996) The Leydig Cell, pp. 241-258. , eds A. H. Payne, M. P. Hardy & L. D. Russell, Cache River Press, Vienna IL
  • Suescun, M.O., Gonzalez, S.I., Chiauzzi, V.A., Calandra, R.S., Effects of induced hypoprolactinemia on resticular function during gonadal maturation in the rat (1985) Journal of Andrology, 6, pp. 77-82
  • Tamarkin, L., Hutchinsnn, J.S., Goldnun, B.D., Regulation of serum gonadotropins by photoperiod and testicular hormone in the Syrian hamster (1976) Endocrinology, 99, pp. 1528-1533
  • Tsuji, M., Takeyama, M., Takatsuka, D., Yabumoto, H., Terada, N., Matsumoto, K., 5α-and 5β-reducrases for 4-ene-3-ketosteroids and 17β-ol-dehydrogenase in epididymis and testis of golden hamster during sexual development (1984) Journal of Steroid Biochemistry, 21, pp. 179-183
  • Turek, F.W., Elliot, J.A., Alvis, J.D., Menaker, M., Effect of prolonged exposure to nonstimulatory photoperiods on the activity of the neuroendocrine-testicular axis of golden hamsters (1975) Bioglogy of Reproduction, 13, pp. 475-481
  • Turek, F.W., Elliot, A., Alvis, J.D., Menaker, M., The interaction of castration and photoperiod in the regulation of hypophyseal and serum gonadotropin levels in male golden hamsters (1975) Endocrinology, 96, pp. 854-860
  • Urbanski, H.F., Doan, A., Pierce, M., Fahrenbach, W.H., Collins, P.M., Maturation of the hypothalamo-pituitary-gonadal axis of male Syrian hamsters (1992) Biology of Reproduction, 46, pp. 991-996
  • Valenti, S., Guido, R., Giusti, M., Giordano, G., In vitro acute and prolonged effects of melatonin on purified rat Leydig cell steroidogenesis and adenosine 3′,5′-monophosphate production (1995) Endocrinology, 136, pp. 5357-5362
  • Vomachka, A.J., Greenwald, G.S., The development of gonadotropin and steroid hormone patterns in male and female hamsters from birth to puberty (1979) Endocrinology, 105, pp. 960-966

Citas:

---------- APA ----------
Frungieri, M.B., Gonzalez-Calvar, S.I., Bartke, A. & Calandra, R.S. (1999) . Influence of age and photoperiod on steroidogenic function of the testis in the golden hamster. International Journal of Andrology, 22(4), 243-252.
http://dx.doi.org/10.1046/j.1365-2605.1999.00175.x
---------- CHICAGO ----------
Frungieri, M.B., Gonzalez-Calvar, S.I., Bartke, A., Calandra, R.S. "Influence of age and photoperiod on steroidogenic function of the testis in the golden hamster" . International Journal of Andrology 22, no. 4 (1999) : 243-252.
http://dx.doi.org/10.1046/j.1365-2605.1999.00175.x
---------- MLA ----------
Frungieri, M.B., Gonzalez-Calvar, S.I., Bartke, A., Calandra, R.S. "Influence of age and photoperiod on steroidogenic function of the testis in the golden hamster" . International Journal of Andrology, vol. 22, no. 4, 1999, pp. 243-252.
http://dx.doi.org/10.1046/j.1365-2605.1999.00175.x
---------- VANCOUVER ----------
Frungieri, M.B., Gonzalez-Calvar, S.I., Bartke, A., Calandra, R.S. Influence of age and photoperiod on steroidogenic function of the testis in the golden hamster. Int. J. Androl. 1999;22(4):243-252.
http://dx.doi.org/10.1046/j.1365-2605.1999.00175.x