Artículo

Gallo, M.; Montserrat, J.M.; Iribarren, A.M. "Design and applications of modified oligonucleotides" (2003) Brazilian Journal of Medical and Biological Research. 36(2):143-151
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Abstract:

Oligonucleotides have a wide range of applications in fields such as biotechnology, molecular biology, diagnosis and therapy. However, the spectrum of uses can be broadened by introducing chemical modifications into their structures. The most prolific field in the search for new oligonucleotide analogs is the antisense strategy, where chemical modifications confer appropriate characteristics such as hybridization, resistance to nucleases, cellular uptake, selectivity and, basically, good pharmacokinetic and pharmacodynamic properties. Combinatorial technology is another research area where oligonucleotides and their analogs are extensively employed. Aptamers, new catalytic ribozymes and deoxyribozymes are RNA or DNA molecules individualized from a randomly synthesized library on the basis of a particular property. They are identified by repeated cycles of selection and amplification, using PCR technologies. Modified nucleotides can be introduced either during the amplification procedure or after selection.

Registro:

Documento: Artículo
Título:Design and applications of modified oligonucleotides
Autor:Gallo, M.; Montserrat, J.M.; Iribarren, A.M.
Filiación:Lab. de Quimica de Acidos Nucleicos, Inst. Invest./Ingenieria Gen. Biol., CONICET-UBA, Vuelta de Obligado 2490,2 Piso, 1428 Buenos Aires, Argentina
Instituto de Ciencias, Univ. Nacional de General Sarmiento, Buenos Aires, Argentina
Centro de Estudios e Investigaciones, Universidad Nacional de Quilmes, Buenos Aires, Argentina
Palabras clave:Antisense; Aptamers; Modified nucleotides; Oligonucleotide libraries; Ribozymes; SELEX method; aptamer; deoxyribozyme; DNA; nuclease; oligonucleotide; ribozyme; RNA; biotechnology; chemical modification; combinatorial library; gene amplification; hybridization; molecular biology; nucleic acid probe; nucleic acid structure; nucleotide transport; polymerase chain reaction; review
Año:2003
Volumen:36
Número:2
Página de inicio:143
Página de fin:151
DOI: http://dx.doi.org/10.1590/S0100-879X2003000200001
Título revista:Brazilian Journal of Medical and Biological Research
Título revista abreviado:Braz. J. Med. Biol. Res.
ISSN:0100879X
CODEN:RBPMB
CAS:deoxyribozyme, 206668-15-7; DNA, 9007-49-2; nuclease, 9026-81-7; RNA, 63231-63-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v36_n2_p143_Gallo

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Citas:

---------- APA ----------
Gallo, M., Montserrat, J.M. & Iribarren, A.M. (2003) . Design and applications of modified oligonucleotides. Brazilian Journal of Medical and Biological Research, 36(2), 143-151.
http://dx.doi.org/10.1590/S0100-879X2003000200001
---------- CHICAGO ----------
Gallo, M., Montserrat, J.M., Iribarren, A.M. "Design and applications of modified oligonucleotides" . Brazilian Journal of Medical and Biological Research 36, no. 2 (2003) : 143-151.
http://dx.doi.org/10.1590/S0100-879X2003000200001
---------- MLA ----------
Gallo, M., Montserrat, J.M., Iribarren, A.M. "Design and applications of modified oligonucleotides" . Brazilian Journal of Medical and Biological Research, vol. 36, no. 2, 2003, pp. 143-151.
http://dx.doi.org/10.1590/S0100-879X2003000200001
---------- VANCOUVER ----------
Gallo, M., Montserrat, J.M., Iribarren, A.M. Design and applications of modified oligonucleotides. Braz. J. Med. Biol. Res. 2003;36(2):143-151.
http://dx.doi.org/10.1590/S0100-879X2003000200001