Artículo

Rodríguez, E.M.; Bigi, R.; Medesani, D.A.; Stella, V.S.; Greco, L.S.L.; Moreno, P.A.R.; Monserrat, J.M.; Pellerano, G.N.; Ansaldo, M. "Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity" (2001) Brazilian Journal of Medical and Biological Research. 34(4):509-518
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Whole body oxygen consumption and some hemolymph parameters such as pH, partial pressure of gases, level of ions and lactate were measured in the estuarine crab Chasmagnathus granulata after both acute (96 h) and chronic (2 weeks) exposure to cadmium at concentrations ranging from 0.4 to 6.3 mg/l. In all instances, the crabs developed hemolymph acidosis, but no respiratory (increased PCO2) or lactate increases were evident. Hemolymph levels of sodium and calcium were always increased by cadmium exposure. The chronic toxicity of cadmium was enhanced at 12%o salinity, even causing a significantly higher mortality in comparison with the higher salinity (30%o) used. A general metabolic arrest took place at 12%o salinity in the crabs chronically exposed to cadmium, as indicated by decreases of oxygen consumption and PCO2, an increase of PO2, along with no changes in lactate levels. These imbalances were associated with severe necrosis and telangiectasia in the respiratory gills, probably leading to respiratory impairment and finally histotoxic hypoxia and death of the animal.

Registro:

Documento: Artículo
Título:Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity
Autor:Rodríguez, E.M.; Bigi, R.; Medesani, D.A.; Stella, V.S.; Greco, L.S.L.; Moreno, P.A.R.; Monserrat, J.M.; Pellerano, G.N.; Ansaldo, M.
Filiación:Departamento de Ciencias Biologicas, FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina
Fund. Univ. do Rio Grande do Sol, Rio Grande, RS, Brazil
Instituto Antarctico Argentino, Buenos Aires, Argentina
Departamento de Ciencias Biologicas, FCEyN, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Blood homeostasis; Cadmium; Crabs; Salinity; cadmium; lactic acid; acidosis; acute toxicity; animal experiment; article; blood toxicity; chasmagnathus granulata; chronic toxicity; concentration (parameters); controlled study; crab; gas analysis; gill; hemolymph; homeostasis; hypoxia; long term exposure; necrosis; nonhuman; oxygen consumption; pH; salinity; telangiectasia
Año:2001
Volumen:34
Número:4
Página de inicio:509
Página de fin:518
DOI: http://dx.doi.org/10.1590/S0100-879X2001000400011
Título revista:Brazilian Journal of Medical and Biological Research
Título revista abreviado:Braz. J. Med. Biol. Res.
ISSN:0100879X
CODEN:RBPMB
CAS:cadmium, 22537-48-0, 7440-43-9; lactic acid, 113-21-3, 50-21-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v34_n4_p509_Rodriguez

Referencias:

  • Boschi, E.E., Los crustáceos decápodos brachyura del litoral bonaerense (1964) Boletin del Instituto de Biología Marina Mar del Plata, 164, pp. 1-34
  • D'Incao, F., Da Silva, K.G., Rufino, M.L., Da Costa Braga, A., Hábito alimentar do caranguejo Chasmagnathus granulata Dana, 1851 na barra do Rio Grande, RS (Decapoda, Grapsidae) (1990) Atlantica, 12, pp. 85-93
  • Menni, R., (1983) Los Peces en el Medio Marino, , Estudio Sigma, Buenos Aires
  • Estudio para la evaluación de la contaminación en el Río de la Plata (1990) Informe de Avance, Argentine Hydrographic Navy Service, , Buenos Aires, Argentina
  • Marcovecchio, J.E., (1988) Estudio Comparativo de la Distribución de Los Metales Traza Mercurio, Cadmio y Zinc en Organismes de Dos Zonas Estuariales de Argentina: Bahia Bianca y Desembocadura del Rio de la Plata, , Doctoral thesis, Universidad de Mar del Plata, Argentina
  • Vitale, A.M., Monserrat, J.M., Castilho, P., Rodriguez, E.M., Inhibitory effects of cadmium on carbonic anhydrase activity and ionic regulation of the estuarine crab Chasmagnathus granulata (Decapoda, Grapsidae) (1999) Comparative Biochemistry and Physiology, 122 C, pp. 121-129
  • Bigi, R., Verrengia-Guerrero, N., Rodriguez, E.M., Kesten, E., Medesani, D.A., Acute lethal toxicity and bioaccumulation of cadmium in the estuarine crab Chasmagnathus granulata (Decapoda, Brachyura) (1996) Pollution Processes in Coastal Environments, pp. 292-295. , Marcovecchio J (Editor), Mar del Plata, Argentina
  • Rand, G.M., (1995) Fundamentals of Aquatic Toxicology. Effects, Environmental Fate, and Risk Assessment. 2nd Edn., , Taylor and Francis, Bristol, UK
  • Rainbow, P.S., Dallinger, R., Metal uptake, regulation and excretion in freshwater invertebrates (1993) Ecotoxicology of Metals in Invertebrates, pp. 119-131. , Dallinger R & Rainbow PS (Editors), Lewis Publishers, Boca Raton
  • Giesy, J.P., Graney, R.L., Recent developments in and intercomparisons of acute and chronic bioassays and bioindicators (1989) Hydrobiologia, 188-189, pp. 21-60
  • Böttcher, K., Siebers, D., Biochemistry, localization and physiology of carbonic anhydrase in the gills of euryhaline crabs (1993) Journal of Experimental Zoology, 265, pp. 397-409
  • Burnett, L.E., Physiological responses to air exposure: Acid-base balance and the role of branchial water stores (1988) American Zoologist, 28, pp. 125-135
  • Henry, R.P., Cameron, J.N., The role of carbonic anhydrase in respiration, ion regulation and acid-base balance in the aquatic crab Callinectes sapidus and the terrestrial crab Gecarcinus lateralis (1983) Journal of Experimental Biology, 103, pp. 205-223
  • Botto, J.L., Yrigoyen, H.R., Biologia de la comunidad del cangrejal. I. Contribución al conocimiento biológico del cangrejo estuarino Chasmagnathus granulata Dana (Crustacea, Decapoda, Grapsidae) en la desembocadura del Rio Salado, provincia de Buenos Aires (1979) Memorias del Seminario Sobre Ecología Bentónica y Sedimentes de la Plataforma Continental del Atlántico Sur, pp. 161-169. , UNESCO (Editor), Montevideo, Uruguay
  • Rossi, J.B., Aspectos hidrobiológicos del Arroyo San Clemente (Bahía Samborombón) (1982) Revista del Museo de la Plata, 132, pp. 29-38
  • Bromberg, E., Santos, E.A., Bianchini, A., Osmotic and ionic regulation in Chasmagnathus granulata Dana, 1851 (Decapoda, Grapsidae) during hyposmotic stress (1995) Nauplius, 3, pp. 83-99
  • O'Hara, J., The influence of temperature and salinity on the toxicity of cadmium to the fiddler crab Uca pugilator (1973) Fisheries Bulletin, 71, pp. 149-153
  • Zaunders, I.P., Rojas, W.E., Salinity effects on cadmium accumulation in various tissues of the tropical fiddler crab Uca rapax (1996) Environmental Pollution, 94, pp. 293-299
  • Boitel, F., Truchot, J.P., Effects of sublethal and lethal copper levels on hemolymph acid-base balance and ion concentrations in the shore crab Carcinus maenas kept in undiluted sea water (1989) Marine Biology, 103, pp. 495-501
  • Tedengren, M., Arnér, M., Kautsky, N., Ecophysiological and stress response of marine and brackish water Gammarus species (Crustacea, Amphipoda) to changes in salinity and exposure to cadmium and diesel-oil (1988) Marine Ecology, Progress Series, 47, pp. 107-116
  • Thurberg, F.P., Dawson, M.A., Collier, R.S., Effects of copper and cadmium on osmoregulation and oxygen consumption in two species of estuarine crabs (1973) Marine Biology, 23, pp. 171-175
  • (1995) Standard Methods for the Examination of Water and Wastewater. 19th Edn., , American Public Health Association, Washington, DC
  • Monserrat, J.M., Rodriguez, E.M., Lombardo, R.J., Effects of salinity on the toxicity of parathion to the estuarine crab Chasmagnathus granulata (Decapoda, Grapsidae) (1991) Bulletin of Environmental Contamination and Toxicology, 46, pp. 569-575
  • Montgomery, D.C., (1984) Design and Analysis of Experiments. 2nd Edn., , John Wiley & Sons, New York
  • Bjerregaard, P., Vislie, T., Effects of cadmium on hemolymph composition in the shore crab Carcinus maenas (1985) Marine Ecology, 27, pp. 135-142
  • Rainbow, P.S., Ecophysiology of trace metal uptake in crustaceans (1997) Estuarine Coastal and Shelf Science, 44, pp. 169-175
  • Stephenson, M., Mackie, G.L., A laboratory study of the effects of waterborne cadmium, calcium, and carbonate concentrations on cadmium concentrations in Hyalella azteca (Crustacea: Amphipoda) (1989) Aquatic Toxicology, 15, pp. 53-62
  • Bubacco, L., Rocco, G.P., Salvato, B., Beltammi, M., The binding of Cd (II) to the hemocyanin of the Mediterranean crab Carcinus maenas (1993) Archives of Biochemistry and Biophysics, 302, pp. 78-84
  • Martin, D.J., Rainbow, P.S., Haemocyanin and the binding of cadmium and zinc in the haemolymph of the shore crab Carcinus maenas (L.) (1998) Science of the Total Environment, 214, pp. 133-152
  • Mangum, C.P., Osmoregulation in marine and estuarine animals, its influence on respiratory gas exchange and transport (1986) Bulletin of Zoology, 53, pp. 1-7
  • Rodríguez Moreno, P.A., Schwarzbaum, P., Rodriguez, E.M., Effects of cadmium on gill Na, K-ATPase of the estuarine crab Chasmagnathus granulata during postmolt: In vivo and in vitro studies (1998) Bulletin of Environmental Contamination and Toxicology, 61, pp. 629-636
  • Hillyard, S.D., Sera, R., Gonick, H.C., Effect of Cd++ on short-circuit current across epithelial membranes (1979) Journal of Membrane Biology, 46, pp. 283-294
  • Silverthorn, D.U., (1998) Human Physiology. An Integrated Approach, , Prentice Hall, New Jersey
  • Nonnotte, L., Boitel, F., Truchot, J.P., Waterborne copper causes gill damage and hemolymph hypoxia in the shore crab Carcinus maenas (1993) Canadian Journal of Zoology, 71, pp. 1569-1576
  • Freitas Rebelo, M., Rodriguez, E.M., Santos, E.A., Ansaldo, M., Histopathological changes in gills of the estuarine crab Chasmagnathus granulata (Crustacea-Decapoda) following acute exposure to ammonia (2000) Comparative Biochemistry and Physiology, 125 C, pp. 157-164
  • Cameron, J.N., Acid-base regulation in fishes. 2. Biological responses and limitations (1989) Acid Toxicity and Aquatic Animals, pp. 99-112. , Morris R, Taylor EW, Brown DJA & Brown JA (Editors), Cambridge University Press, Cambridge, UK
  • Boitel, F., Truchot, J.P., Comparative study of the effects of copper on haemolymph ion concentrations and acid-base balance in shore crabs Carcinus maenas acclimated to full-strength or dilute seawater (1990) Comparative Biochemistry and Physiology, 95 C, pp. 307-312

Citas:

---------- APA ----------
Rodríguez, E.M., Bigi, R., Medesani, D.A., Stella, V.S., Greco, L.S.L., Moreno, P.A.R., Monserrat, J.M.,..., Ansaldo, M. (2001) . Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity. Brazilian Journal of Medical and Biological Research, 34(4), 509-518.
http://dx.doi.org/10.1590/S0100-879X2001000400011
---------- CHICAGO ----------
Rodríguez, E.M., Bigi, R., Medesani, D.A., Stella, V.S., Greco, L.S.L., Moreno, P.A.R., et al. "Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity" . Brazilian Journal of Medical and Biological Research 34, no. 4 (2001) : 509-518.
http://dx.doi.org/10.1590/S0100-879X2001000400011
---------- MLA ----------
Rodríguez, E.M., Bigi, R., Medesani, D.A., Stella, V.S., Greco, L.S.L., Moreno, P.A.R., et al. "Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity" . Brazilian Journal of Medical and Biological Research, vol. 34, no. 4, 2001, pp. 509-518.
http://dx.doi.org/10.1590/S0100-879X2001000400011
---------- VANCOUVER ----------
Rodríguez, E.M., Bigi, R., Medesani, D.A., Stella, V.S., Greco, L.S.L., Moreno, P.A.R., et al. Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity. Braz. J. Med. Biol. Res. 2001;34(4):509-518.
http://dx.doi.org/10.1590/S0100-879X2001000400011