Artículo

Chasseing, N.A.; Hofer, E.; Bordenave, R.H.; Shanley, C.; Rumi, L.S. "Bone marrow fibroblasts in patients with advanced lung cancer" (2001) Brazilian Journal of Medical and Biological Research. 34(11):1457-1463
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Abstract:

In a previous study we demonstrated that the incidence of fibroblast colony-forming units (CFU-F) was very low in bone marrow primary cultures from the majority of untreated advanced non-small lung cancer patients (LCP) compared to normal controls (NC). For this reason, we studied the ability of bone marrow stromal cells to achieve confluence in primary cultures and their proliferative capacity following four continuous subcultures in consecutive untreated LCP and NC. We also evaluated the production of interleukin-1ß (IL-1ß) and prostaglandin E2 (PGE2) by pure fibroblasts. Bone marrow was obtained from 20 LCP and 20 NC. A CFU-F assay was used to investigate the proliferative and confluence capacity. Levels of IL-1ß and PGE2 in conditioned medium (CM) of pure fibroblast cultures were measured with an ELISA kit and RIA kit, respectively. Only fibroblasts from 6/13 (46%) LCP confluent primary cultures had the capacity to proliferate following four subcultures (NC = 100%). Levels of spontaneously released IL-1ß were below 10 pg/ml in the CM of LCP, while NC had a mean value of 1,217 ± 74 pg/ml. In contrast, levels of PGE2 in these CM of LCP were higher (77.5 ± 23.6 pg/ml) compared to NC (18.5 ± 0.9 pg/ml). In conclusion, bone marrow fibroblasts from LCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1ß and PGE2 production.

Registro:

Documento: Artículo
Título:Bone marrow fibroblasts in patients with advanced lung cancer
Autor:Chasseing, N.A.; Hofer, E.; Bordenave, R.H.; Shanley, C.; Rumi, L.S.
Filiación:Res. Member of the Consejo Nac. de Invest. Cie. y Tecnicas de la Republica Argentina (CONICET), Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Buenos Aires, Argentina
Departamento de Oncologia, I. Iriarte Hospital, Buenos Aires, Argentina
Departamento de Hematologia e Transplante de Médula Óssea, Hospital Británico, Buenos Aires, Argentina
Instituto de Biología y Medicina Experimental, Vuelta de Obligado, 2490, 1428 Buenos Aires, Argentina
Palabras clave:Cancer; Fibroblasts; Interleukin-1; Prostaglandin E2; interleukin 1beta; prostaglandin E2; adult; advanced cancer; article; bone marrow cell; bone marrow culture; cancer patient; cell assay; cell proliferation; clinical article; colony forming unit F; controlled study; culture medium; cytokine production; enzyme linked immunosorbent assay; fibroblast; fibroblast culture; human; human cell; lung cancer; lung non small cell cancer; prostaglandin synthesis; radioimmunoassay; stroma cell
Año:2001
Volumen:34
Número:11
Página de inicio:1457
Página de fin:1463
DOI: http://dx.doi.org/10.1590/S0100-879X2001001100014
Título revista:Brazilian Journal of Medical and Biological Research
Título revista abreviado:Braz. J. Med. Biol. Res.
ISSN:0100879X
CODEN:RBPMB
CAS:prostaglandin E2, 363-24-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v34_n11_p1457_Chasseing

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Citas:

---------- APA ----------
Chasseing, N.A., Hofer, E., Bordenave, R.H., Shanley, C. & Rumi, L.S. (2001) . Bone marrow fibroblasts in patients with advanced lung cancer. Brazilian Journal of Medical and Biological Research, 34(11), 1457-1463.
http://dx.doi.org/10.1590/S0100-879X2001001100014
---------- CHICAGO ----------
Chasseing, N.A., Hofer, E., Bordenave, R.H., Shanley, C., Rumi, L.S. "Bone marrow fibroblasts in patients with advanced lung cancer" . Brazilian Journal of Medical and Biological Research 34, no. 11 (2001) : 1457-1463.
http://dx.doi.org/10.1590/S0100-879X2001001100014
---------- MLA ----------
Chasseing, N.A., Hofer, E., Bordenave, R.H., Shanley, C., Rumi, L.S. "Bone marrow fibroblasts in patients with advanced lung cancer" . Brazilian Journal of Medical and Biological Research, vol. 34, no. 11, 2001, pp. 1457-1463.
http://dx.doi.org/10.1590/S0100-879X2001001100014
---------- VANCOUVER ----------
Chasseing, N.A., Hofer, E., Bordenave, R.H., Shanley, C., Rumi, L.S. Bone marrow fibroblasts in patients with advanced lung cancer. Braz. J. Med. Biol. Res. 2001;34(11):1457-1463.
http://dx.doi.org/10.1590/S0100-879X2001001100014