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Baldi, P.C.; Velikovsky, C.A.; Braden, B.C.; Giambartolomei, G.H.; Fossati, C.A.; Goldbaum, F.A. "Structural, functional and immunological studies on a polymeric bacterial protein" (2000) Brazilian Journal of Medical and Biological Research. 33(7):741-747
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Abstract:

The characterization of proteins from Brucella spp, the causative agent of brucellosis, has been the subject of intensive research. We have described an 18-kDa cytoplasmic protein of Brucella abortus and shown the potential usefulness of this protein as an antigen for the serologic diagnosis of brucellosis. The amino acid sequence of the protein showed a low but significant homology with that of lumazine synthases. Lumazine is an intermediate product in bacterial riboflavin biosynthesis. The recombinant form of the 18-kDa protein (expressed in E. coli) folds like the native Brucella protein and has lumazine-synthase enzymatic activity. Three-dimensional analysis by X-ray crystallography of the homolog Bacillus subtilis lumazine synthase has revealed that the enzyme forms an icosahedral capsid. Recombinant lumazine synthase from B. abortus was crystallized, diffracted X rays to 2.7-Å resolution at room temperature, and the structure successfully solved by molecular replacement procedures. The macromolecular assembly of the enzyme differs from that of the enzyme from B. subtilis. The Brucella enzyme remains pentameric (90 kDa) in its crystallographic form. Nonetheless, the active sites of the two enzymes are virtually identical at the structural level, indicating that inhibitors of these enzymes could be viable pharmaceuticals across a broad species range. We describe the structural reasons for the differences in their quaternary arrangement and also discuss the potential use of this protein as a target for the development of acellular vaccines.

Registro:

Documento: Artículo
Título:Structural, functional and immunological studies on a polymeric bacterial protein
Autor:Baldi, P.C.; Velikovsky, C.A.; Braden, B.C.; Giambartolomei, G.H.; Fossati, C.A.; Goldbaum, F.A.
Filiación:Inst. Estud. de la Inmunidad Humoral, Fac. de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Natural Sciences, Bowie State University, Bowei, MD, United States
Inst. de Invest. Bioquímicas, Fundación Campomar, BBA-CONICET,FCEN-UBA, Buenos Aires, Argentina
Inst. de Invest. Bioquímicas, Fundación Campomar, Av. Patricias Argentinas, 435, Buenos Aires 1405, Argentina
Palabras clave:Bacillus subtilis lumazine synthase; Brucella lumazine synthase; Brucellosis detection; Immunogenicity; X-ray structure; Bacillus subtilis; Bacteria (microorganisms); Brucella; Brucella melitensis biovar Abortus; Miridae; 6,7 dimethyl 8 ribityllumazine synthase; 6,7-dimethyl-8-ribityllumazine synthase; bacterial antigen; Brucella vaccine; lipoprotein; multienzyme complex; OMP19 protein, Brucella abortus; outer membrane protein; recombinant protein; affinity chromatography; animal; article; Brucella abortus; brucellosis; chemistry; crystallography; enzyme linked immunosorbent assay; enzymology; human; immunology; protein quaternary structure; protein tertiary structure; Animals; Antigens, Bacterial; Bacterial Outer Membrane Proteins; Brucella abortus; Brucella Vaccine; Brucellosis; Chromatography, Affinity; Crystallography; Enzyme-Linked Immunosorbent Assay; Humans; Lipoproteins; Multienzyme Complexes; Protein Structure, Quaternary; Protein Structure, Tertiary; Recombinant Proteins
Año:2000
Volumen:33
Número:7
Página de inicio:741
Página de fin:747
DOI: http://dx.doi.org/10.1590/S0100-879X2000000700003
Título revista:Brazilian Journal of Medical and Biological Research
Título revista abreviado:Braz. J. Med. Biol. Res.
ISSN:0100879X
CODEN:RBPMB
CAS:6,7-dimethyl-8-ribityllumazine synthase, 89287-46-7; Antigens, Bacterial; Bacterial Outer Membrane Proteins; Brucella Vaccine; Lipoproteins; Multienzyme Complexes; OMP19 protein, Brucella abortus; Recombinant Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v33_n7_p741_Baldi

Referencias:

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Citas:

---------- APA ----------
Baldi, P.C., Velikovsky, C.A., Braden, B.C., Giambartolomei, G.H., Fossati, C.A. & Goldbaum, F.A. (2000) . Structural, functional and immunological studies on a polymeric bacterial protein. Brazilian Journal of Medical and Biological Research, 33(7), 741-747.
http://dx.doi.org/10.1590/S0100-879X2000000700003
---------- CHICAGO ----------
Baldi, P.C., Velikovsky, C.A., Braden, B.C., Giambartolomei, G.H., Fossati, C.A., Goldbaum, F.A. "Structural, functional and immunological studies on a polymeric bacterial protein" . Brazilian Journal of Medical and Biological Research 33, no. 7 (2000) : 741-747.
http://dx.doi.org/10.1590/S0100-879X2000000700003
---------- MLA ----------
Baldi, P.C., Velikovsky, C.A., Braden, B.C., Giambartolomei, G.H., Fossati, C.A., Goldbaum, F.A. "Structural, functional and immunological studies on a polymeric bacterial protein" . Brazilian Journal of Medical and Biological Research, vol. 33, no. 7, 2000, pp. 741-747.
http://dx.doi.org/10.1590/S0100-879X2000000700003
---------- VANCOUVER ----------
Baldi, P.C., Velikovsky, C.A., Braden, B.C., Giambartolomei, G.H., Fossati, C.A., Goldbaum, F.A. Structural, functional and immunological studies on a polymeric bacterial protein. Braz. J. Med. Biol. Res. 2000;33(7):741-747.
http://dx.doi.org/10.1590/S0100-879X2000000700003