Artículo

Malnic, G.; Ansaldo, M.; Lantos, C.P.; Damasco, M.C. "Regulation of nephron acidification by corticosteroids" (1997) Brazilian Journal of Medical and Biological Research. 30(4):479-486
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Abstract:

The present paper reviews work from our laboratories evaluating the importance of adrenal cortical hormones in acidification by proximal and cortical distal tubules. Proximal acidification was determined by stationary microperfusion, and measurement of bicarbonate reabsorption using luminal pH determination was performed with H+-ion-sensitive microelectrodes. Rats were adrenalectomized (ADX) 48 h before the experiments, and corticosteroids (aldosterone (A), corticosterone (B), and 18-OH corticosterone (18-OH-B)) were injected intramuscularly 100 and 40 min before the experiments. In ADX rats stationary pH increased significantly to 7.03 as compared to sham-operated rats (6.78). Bicarbonate reabsorption decreased from 2.65 ± 0.18 in sham-operated rats to 0.50 ± 0.07 nmol cm-2 s-1 after ADX. The administration of the three hormones stimulated proximal tubule acidification, reaching, however, only 47.2% of the sham values in aldosterone-treated rats. Distal nephron acidification was studied by measuring urine minus blood pCO2 differences (U-B pCO2) in bicarbonate-loaded rats treated as above. This pCO2 difference is used as a measure of the distal nephron ability to secrete H+ ions into an alkaline urine. U-B pCO2 decreased significantly from 39.9 ± 1.26 to 11.9 ± 1.99 mmHg in ADX rats. When corticosteroids were given to ADX rats before the experiment, U-B pCO2 increased significantly, but reached control levels only when aldosterone (two 3-μg doses per rat) plus corticosterone (220 μg) were given together. In order to control for the effect of aldosterone on distal transepithelial potential difference one group of rats was treated with amiloride, which blocks distal sodium channels. Amiloride-treated rats still showed a significant reduction in U-B pCO2 after ADX. Only corticosterone and 18-OH-B but not aldosterone increased U-B pCO2 back to the levels of sham-operated rats. These results show that corticosteroids stimulate renal tubule acidification both in proximal and distal nephrons and provide some clues about the mechanism of action of these steroids.

Registro:

Documento: Artículo
Título:Regulation of nephron acidification by corticosteroids
Autor:Malnic, G.; Ansaldo, M.; Lantos, C.P.; Damasco, M.C.
Filiación:Depto. de Fisiologia e Bioffsica, Inst. de Cie. Biomédicas, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil
Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Depto. de Fisiol. e Biofísica, ICB, USP, Av. Prof. Lineu Prestes, 1524, 05508-900 Sao Paulo, SP, Brazil
Palabras clave:aldosterone; amiloride; bicarbonate reabsorption; corticosterone; pCO2; urine; 18 hydroxycorticosterone; aldosterone; amiloride; bicarbonate; carbon dioxide; corticosteroid; corticosterone; acidification; adrenalectomy; animal experiment; conference paper; controlled study; intramuscular drug administration; nephron; nonhuman; rat; urine; Animalia
Año:1997
Volumen:30
Número:4
Página de inicio:479
Página de fin:486
DOI: http://dx.doi.org/10.1590/S0100-879X1997000400008
Título revista:Brazilian Journal of Medical and Biological Research
Título revista abreviado:BRAZ. J. MED. BIOL. RES.
ISSN:0100879X
CODEN:RBPMB
CAS:18 hydroxycorticosterone, 561-65-9; aldosterone, 52-39-1, 6251-69-0; amiloride, 2016-88-8, 2609-46-3; bicarbonate, 144-55-8, 71-52-3; carbon dioxide, 124-38-9, 58561-67-4; corticosterone, 50-22-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v30_n4_p479_Malnic

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Citas:

---------- APA ----------
Malnic, G., Ansaldo, M., Lantos, C.P. & Damasco, M.C. (1997) . Regulation of nephron acidification by corticosteroids. Brazilian Journal of Medical and Biological Research, 30(4), 479-486.
http://dx.doi.org/10.1590/S0100-879X1997000400008
---------- CHICAGO ----------
Malnic, G., Ansaldo, M., Lantos, C.P., Damasco, M.C. "Regulation of nephron acidification by corticosteroids" . Brazilian Journal of Medical and Biological Research 30, no. 4 (1997) : 479-486.
http://dx.doi.org/10.1590/S0100-879X1997000400008
---------- MLA ----------
Malnic, G., Ansaldo, M., Lantos, C.P., Damasco, M.C. "Regulation of nephron acidification by corticosteroids" . Brazilian Journal of Medical and Biological Research, vol. 30, no. 4, 1997, pp. 479-486.
http://dx.doi.org/10.1590/S0100-879X1997000400008
---------- VANCOUVER ----------
Malnic, G., Ansaldo, M., Lantos, C.P., Damasco, M.C. Regulation of nephron acidification by corticosteroids. BRAZ. J. MED. BIOL. RES. 1997;30(4):479-486.
http://dx.doi.org/10.1590/S0100-879X1997000400008