Artículo

Tortorella, H.; Konrath, R.A.; Mazzini, M.N.; Brandelli, A. "Proteolytic release and partial characterization of human sperm-surface glycopeptides" (1997) Brazilian Journal of Medical and Biological Research. 30(3):387-393
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Abstract:

Sperm-surface glycopeptides were obtained from intact sperm membranes after proteolytic release by different enzymatic treatments such as autoproteolysis, trypsin, papain and pronase. Glycopeptides were isolated, their properties and composition were examined, and their monosaccharide and amino acid constituents were characterized. The monosaccharides identified were fucose, mannose, galactose, N-acetylglucosamine, and N-acetylgalactosamine, which form part of more than one type of oligosaccharide units. Autoproteolytic treatment mainly provided O-glycosidic type oligosaccharides, while a mixture of O- and N-glycosidic oligosaccharides was obtained in variable proportions when treated with trypsin, papain or pronase. The highest degree of peptide cleavage was obtained with pronase. Despite the higher yields reached with trypsin, these glycopeptides contain the lowest percentage of oligosaccharide chains. Proteolytic treatment provides a simple, rapid procedure for the isolation of glycopeptides from the sperm surface.

Registro:

Documento: Artículo
Título:Proteolytic release and partial characterization of human sperm-surface glycopeptides
Autor:Tortorella, H.; Konrath, R.A.; Mazzini, M.N.; Brandelli, A.
Filiación:Lab. Fertilidade Esterilidade Humana, Departamento de Bioquímica, Univ. Federal do Rio Grande do Sul, 90050-170 Porto Alegre, RS, Brazil
Depto. de Quimica Orgânica, Fac. de Cie. Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
ICTA, UFRGS, Av. Bento Conçalves, 9500, 91501-970 Porto Alegre, RS, Brazil
Palabras clave:Carbohydrate; Glycoconjugate; Human sperm; Peptide; Proteolysis; glycopeptide; papain; peptide hydrolase; pronase; trypsin; article; human; male; metabolism; spermatozoon; Glycopeptides; Humans; Male; Papain; Peptide Hydrolases; Pronase; Spermatozoa; Trypsin
Año:1997
Volumen:30
Número:3
Página de inicio:387
Página de fin:393
DOI: http://dx.doi.org/10.1590/S0100-879X1997000300013
Título revista:Brazilian Journal of Medical and Biological Research
Título revista abreviado:Braz. J. Med. Biol. Res.
ISSN:0100879X
CODEN:RBPMB
CAS:Glycopeptides; Papain, EC 3.4.22.2; Peptide Hydrolases, EC 3.4.-; Pronase, EC 3.4.24.-; Trypsin, EC 3.4.21.4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v30_n3_p387_Tortorella

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Citas:

---------- APA ----------
Tortorella, H., Konrath, R.A., Mazzini, M.N. & Brandelli, A. (1997) . Proteolytic release and partial characterization of human sperm-surface glycopeptides. Brazilian Journal of Medical and Biological Research, 30(3), 387-393.
http://dx.doi.org/10.1590/S0100-879X1997000300013
---------- CHICAGO ----------
Tortorella, H., Konrath, R.A., Mazzini, M.N., Brandelli, A. "Proteolytic release and partial characterization of human sperm-surface glycopeptides" . Brazilian Journal of Medical and Biological Research 30, no. 3 (1997) : 387-393.
http://dx.doi.org/10.1590/S0100-879X1997000300013
---------- MLA ----------
Tortorella, H., Konrath, R.A., Mazzini, M.N., Brandelli, A. "Proteolytic release and partial characterization of human sperm-surface glycopeptides" . Brazilian Journal of Medical and Biological Research, vol. 30, no. 3, 1997, pp. 387-393.
http://dx.doi.org/10.1590/S0100-879X1997000300013
---------- VANCOUVER ----------
Tortorella, H., Konrath, R.A., Mazzini, M.N., Brandelli, A. Proteolytic release and partial characterization of human sperm-surface glycopeptides. Braz. J. Med. Biol. Res. 1997;30(3):387-393.
http://dx.doi.org/10.1590/S0100-879X1997000300013