Artículo

de Lederkremer, R.M. "Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi." (1994) Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]. 27(2):239-242
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Abstract:

Two glycoinositol phospholipids (GIPL A and GIPL B) have been purified from epimastigotes of Trypanosoma cruzi at the logarithmic phase of growth (2 days). The GIPLs differ mainly in the lipid moiety and are similar to the lipopeptidophosphoglycan (LPPG) previously isolated from epimastigotes at the stationary phase (4-5 days). [3H]-palmitic acid was incorporated into 1-O-hexadecyl-2-O-palmitoylglycerol in GIPL A and into a sphinganine ceramide with palmitic acid and lignoceric acid as the fatty acids in GIPL B. The lipids could be released by incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) or glycosylphosphatidylinositol phospholipase D (GPI-PLD) from rat serum. The oligosaccharides share the common core structure of the glycosylphosphatidylinositol (GPI) membrane anchors. Microheterogeneity was demonstrated, as well as substitution by galactose, which is mainly in the furanose configuration as was previously described for the LPPG. However, methylation analysis indicated that 20% of the galactose is present as terminal pyranose units. In infective trypomastigotes, [3H]-palmitic acid was incorporated into the anchor of the Tc-85 glycoprotein. The lipid cleaved by phospholipase C digestion was identified as 1-O-hexadecylglycerol and the main oligosaccharide has the structure of the conserved core of all GPI anchors. [3H]-palmitic acid-labelled Tc-85 released into the culture medium as membrane vesicles showed 80% resistance to the action of PI-PLC. However, after mild alkaline hydrolysis, part of the radioactivity was released by the enzyme.

Registro:

Documento: Artículo
Título:Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi.
Autor:de Lederkremer, R.M.
Filiación:Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:fatty acid; glycolipid; glycosylphosphatidylinositol; phosphatidylinositol; protozoal protein; animal; article; carbohydrate analysis; chemistry; metabolism; molecular genetics; Trypanosoma cruzi; Animals; Carbohydrate Sequence; Fatty Acids; Glycolipids; Glycosylphosphatidylinositols; Molecular Sequence Data; Phosphatidylinositols; Protozoan Proteins; Trypanosoma cruzi
Año:1994
Volumen:27
Número:2
Página de inicio:239
Página de fin:242
Título revista:Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]
Título revista abreviado:Braz. J. Med. Biol. Res.
ISSN:0100879X
CAS:Fatty Acids; Glycolipids; Glycosylphosphatidylinositols; Phosphatidylinositols; Protozoan Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v27_n2_p239_deLederkremer

Citas:

---------- APA ----------
(1994) . Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi. Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], 27(2), 239-242.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v27_n2_p239_deLederkremer [ ]
---------- CHICAGO ----------
de Lederkremer, R.M. "Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi." Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] 27, no. 2 (1994) : 239-242.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v27_n2_p239_deLederkremer [ ]
---------- MLA ----------
de Lederkremer, R.M. "Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi." Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], vol. 27, no. 2, 1994, pp. 239-242.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v27_n2_p239_deLederkremer [ ]
---------- VANCOUVER ----------
de Lederkremer, R.M. Free and protein-linked glycoinositolphospholipids in Trypanosoma cruzi. Braz. J. Med. Biol. Res. 1994;27(2):239-242.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v27_n2_p239_deLederkremer [ ]