A hydrophobic fraction isolated from trypomastigotes of Trypanosoma cruzi is being characterized using immunological and chemical techniques. The lipopeptidophosphoglycan (LPPG) was identified in this fraction since it gave a positive reaction with anti-LPPG rabbit serum and had similar structural features such as the presence of ceramide as the lipid moiety, furanoic galactose, and a glycan moiety consistent with that obtained from an authentic sample of epimastigote LPPG, as judged by thin-layer chromatography. Furthermore, the hydrophobic fraction contained other glycolipids with different structural features. The lipid moiety of these compounds is alkylglycerol rather than a ceramide, the carbohydrate chain appears to be less complex than that in LPPG and no reactivity was observed towards an anti-LPPG serum.
Documento: | Artículo |
Título: | Structural features of LPPG and related compounds isolated from Trypanosoma cruzi trypomastigotes. |
Autor: | Casal, O.L.; Zingales, B.; Colli, W. |
Filiación: | Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
Palabras clave: | glycoconjugate; peptidoglycan; phospholipid; protozoal protein; animal; article; carbohydrate analysis; chemistry; molecular genetics; thin layer chromatography; Trypanosoma cruzi; Animals; Carbohydrate Sequence; Chromatography, Thin Layer; Glycoconjugates; Molecular Sequence Data; Peptidoglycan; Phospholipids; Protozoan Proteins; Trypanosoma cruzi |
Año: | 1994 |
Volumen: | 27 |
Número: | 2 |
Página de inicio: | 227 |
Página de fin: | 231 |
Título revista: | Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] |
Título revista abreviado: | Braz. J. Med. Biol. Res. |
ISSN: | 0100879X |
CAS: | peptidoglycan, 9047-10-3; Glycoconjugates; Peptidoglycan; Phospholipids; Protozoan Proteins |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100879X_v27_n2_p227_Casal |