Artículo

Piola, L.; Fuchs, J.; Oneto, M.L.; Basack, S.; Giménez, R.; Massaro, R.; Papa, J.C.; Kesten, E.; Casabé, N. "Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters " (2009) Pesquisa Agropecuaria Brasileira. 44(8):874-880
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Abstract:

The objective of this work was to evaluate the effects of chlorpyrifos on earthworms and on soil functional parameters. An integrated laboratory-field study was performed in a wheat fi eld in Argentina, sprayed with chlorpyrifos at two recommended application rates (240 or 960 g ha -1 a.i.). Laboratory tests included neutral red retention time, comet assay (single cell gel electrophoresis), and avoidance behavior, each using the earthworm Eisenia andrei exposed in soil collected 1 or 14 days after pesticide application, and the bait-lamina test. Field tests assessed organic matter breakdown using the litterbag and bait-lamina assays. Earthworm populations in the field were assessed using formalin application and hand-sorting. The neutral red retention time and comet assays were sensitive biomarkers to the effects of chlorpyrifos on the earthworm E. andrei; however, the earthworm avoidance test was not sufficiently robust to assess these effects. Feeding activity of soil biota, assessed by the bait lamina test, was significantly inhibited by chlorpyrifos after 97 days, but recovered by the 118th day of the test. Litterbag test showed no significant differences in comparison to controls. Earthworm abundance in the fi eld was too low to adequately test the sensitivity of this assessment endpoint.

Registro:

Documento: Artículo
Título:Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters
Autor:Piola, L.; Fuchs, J.; Oneto, M.L.; Basack, S.; Giménez, R.; Massaro, R.; Papa, J.C.; Kesten, E.; Casabé, N.
Filiación:Universidad de Buenos Aires (UBA), Fac. de Ciencias Exactas y Naturales (FCEN), Depart. de Química Biológica (DQB), Toxicología y Química Legal, Ciudad Universitaria, Pabellón 2, 4 piso, 1428 Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Toxicología y Química Legal, UBA, FCEN, DQB, Ciudad Universitaria, Pabellón 2, 4 piso, 1428 Buenos Aires, Argentina
UBA, Facultad de Agronomía, Area de Protección Vegetal, Avenida San Martín 4453, 1417 Buenos Aires, Argentina
Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Oliveros, Ruta 11, Km 353, 2206 Oliveros, Santa Fe, Argentina
Palabras clave:Agricultural soil; Eisenia andrei; Field assay; Laboratory bioassay; Eisenia andrei; Triticum aestivum
Año:2009
Volumen:44
Número:8
Página de inicio:874
Página de fin:880
DOI: http://dx.doi.org/10.1590/S0100-204X2009000800011
Handle:http://hdl.handle.net/20.500.12110/paper_0100204X_v44_n8_p874_Piola
Título revista:Pesquisa Agropecuaria Brasileira
Título revista abreviado:Pesqui. Agropecu. Bras.
ISSN:0100204X
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0100204X_v44_n8_p874_Piola.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100204X_v44_n8_p874_Piola

Referencias:

  • Casabé, N., Piola, L., Fuchs, J., Oneto, M.L., Pamparato, L., Basack, S., Giménez, R., Kesten, E., Ecotoxicological assessment of the effects of glyphosate and chlorpyrifos in an Argentine soya field (2007) Journal of Soils and Sediments, 7, pp. 232-239
  • Colgan, A., Hankard, P.K., Spurgeon, D.J., Svendsen, C., Wadsworth, R.A., Weeks, J.M., Closing the loop: a spatial analysis to link observed environmental damage to predicted heavy metal emissions (2003) Environmental Toxicology and Chemistry, 22, pp. 970-976
  • da Luz, T.N., Ribeiro, R., Sousa, J.P., Avoidance tests with collembola and earthworms as early screening tools for site-specific assessment of polluted soils (2004) Environmental Toxicology and Chemistry, 23, pp. 2188-2193
  • Filser, J., Koehler, H., Ruf, A., Römbke, J., Prinzing, A., Schaefer, M., Ecological theory meets soil ecotoxicology: challenge and chance (2008) Basic and Applied Ecology, 9, pp. 346-355
  • Förster, B., van Gestel, C., Koolhaas, J.E., Nentwig, G., Rodrigues, J.M.L., Sousa, J.P., Jones, S.E., Knacker, T., Ring testing and field-validation of a terrestrial model ecosystem (TME) - an instrument for testing potentially harmful substances: effects of carbendazim on organic matter breakdown and soil fauna feeding activity (2004) Ecotoxicology, 13, pp. 129-141
  • Fourie, F., Reinecke, S.A., Reinecke, A.J., The determination of earthworm species sensitivity differences to cadmium genotoxicity using the comet assay (2007) Ecotoxicology and Environmental Safety, 67, pp. 361-368
  • Gastaldi, L., Ranzato, E., Caprì, F., Hankard, P., Pérès, G., Canesi, L., Viarengo, A., Pons, G., Application of a biomarker battery for the evaluation of the sublethal effects of pollutants in the earthworm Eisenia andrei (2007) Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 146, pp. 398-405
  • Helling, B., Pfeiff, G., Larink, O., A comparison of feeding activity of collembolan and enchytraeid in laboratory studies using the bait-lamina test (1998) Applied Soil Ecology, 7, pp. 207-212
  • Hodge, S., Webster, K.M., Booth, L., Hepplethwaite, V., O'halloran, K., Non-avoidance of organophosphate insecticides by the earthworm Aporrectodea caliginosa (Lumbricidae) (2000) Soil Biology & Biochemistry, 32, pp. 425-428
  • Hund-Rinke, K., Achazi, R.K., Römbke, J., Warnecke, D., Avoidance test with Eisenia fetida as indicator for the habitat function of soils: results of a laboratory comparison test (2003) Journal of Soils and Sediments, 3, pp. 7-12
  • Ingelsfield, C., Pyrethroids and terrestrial non-target organisms (1989) Pesticide Science, 27, pp. 387-428
  • (2008) ISO 17512-1: soil quality - avoidance test for determining the quality of soils and effects of chemicals on behaviour - Part 1: test with earthworms (Eisenia fetida and Eisenia andrei), , International Organization for Standardization. Geneve: ISO
  • Knacker, T., Förster, B., Römbke, J., Frampton, G.K., Assessing the effects of plant protection products on organic matter breakdown in arable fields-litter decomposition test systems (2003) Soil Biology & Biochemistry, 35, pp. 1269-1287
  • Marques, C., Pereira, R., Gonçalves, F., Using earthworm avoidance behaviour to assess the toxicity of formulated herbicides and their active ingredients on natural soils (2009) Journal of Soils and Sediments, 9, pp. 137-147
  • Martin, F.L., Piearce, T.G., Hewer, A., Phillips, D.H., Semple, K.T., A biomarker model of sublethal genotoxicity (DNA single-strand breaks and adducts) using the sentinel organism Aporrectodea longa in spiked soil (2005) Environmental Pollution, 138, pp. 307-315
  • (1984) Guideline for testing of chemicals, n° 207: earthworms, acute toxicity test, , Organization for Economic Cooperation and Development. Paris: OECD
  • Reinecke, S.A., Reinecke, A.J., Biomarker response and biomass change of earthworms exposed to chlorpyrifos in microcosms (2007) Ecotoxicology and Environmental Safety, 66, pp. 92-101
  • Römbke, J., Breure, A.M., Mulder, C., Rutgers, M., Legislation and ecological quality assessment of soil: implementation of ecological indication systems in Europe (2005) Ecotoxicology and Environmental Safety, 62, pp. 201-210
  • Sousa, A., Pereira, R., Antunes, S.C., Cachada, A., Pereira, E., Duarte, A.C., Gonçalves, F., Validation of avoidance assays for the screening assessment of soils under different anthropogenic disturbances (2008) Ecotoxicology and Environmental Safety, 71, pp. 661-670
  • Suthar, S., Earthworm communities a bioindicator of arable land management practices: a case study in semiarid region of India (2009) Ecological Indicators, 9, pp. 588-594
  • Svendsen, C., Spurgeon, D.J., Hankard, P.K., Weeks, J.M., A review of lysosomal membrane stability measured by neutral red retention: is it a workable earthworm biomarker? (2004) Ecotoxicology and Environmental Safety, 57, pp. 20-29
  • Vasseur, P., Cossu-Leguille, C., Biomarkers and community indices as complementary tools for environmental safety (2003) Environment International, 28, pp. 711-717
  • von Törne, E., Assessing feeding activities of soil-living animals: bait-lamina tests (1990) Pedobiologia, 34, pp. 89-101
  • Weeks, J.M., Svendsen, C., Neutral red retention by lysosomes from earthworm (Lumbricus rubellus) coelomocytes: a simple biomarker of exposure to soil copper (1996) Environmental Toxicology and Chemistry, 15, pp. 1801-1805
  • Xiao, N.W., Song, Y., Ge, F., Liu, X.H., Ou-Yang, Z.Y., Biomarkers responses of the earthworm Eisenia fetida to acetochlor exposure in OECD soil (2006) Chemosphere, 65, pp. 907-912
  • Yu, Y.L., Wu, X.M., Li, S.N., Fang, H., Zhan, H.Y., Yu, J.Q., An exploration of the relationship between adsorption and bioavailability of pesticides in soil to earthworm (2006) Environmental Pollution, 141, pp. 428-433
  • Zhou, S., Duan, C., Fu, H., Chen, Y., Wang, X., Yu, Z., Toxicity assessment for chlorpyrifos-contaminated soil with three different earthworm test methods (2007) Journal of Environmental Sciences, 19, pp. 854-858

Citas:

---------- APA ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Giménez, R., Massaro, R., Papa, J.C.,..., Casabé, N. (2009) . Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters . Pesquisa Agropecuaria Brasileira, 44(8), 874-880.
http://dx.doi.org/10.1590/S0100-204X2009000800011
---------- CHICAGO ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Giménez, R., Massaro, R., et al. "Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters " . Pesquisa Agropecuaria Brasileira 44, no. 8 (2009) : 874-880.
http://dx.doi.org/10.1590/S0100-204X2009000800011
---------- MLA ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Giménez, R., Massaro, R., et al. "Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters " . Pesquisa Agropecuaria Brasileira, vol. 44, no. 8, 2009, pp. 874-880.
http://dx.doi.org/10.1590/S0100-204X2009000800011
---------- VANCOUVER ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Giménez, R., Massaro, R., et al. Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters . Pesqui. Agropecu. Bras. 2009;44(8):874-880.
http://dx.doi.org/10.1590/S0100-204X2009000800011