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Abstract:

The objective of this work was to evaluate the effects of chlorpyrifos on earthworms and on soil functional parameters. An integrated laboratory-field study was performed in a wheat fi eld in Argentina, sprayed with chlorpyrifos at two recommended application rates (240 or 960 g ha -1 a.i.). Laboratory tests included neutral red retention time, comet assay (single cell gel electrophoresis), and avoidance behavior, each using the earthworm Eisenia andrei exposed in soil collected 1 or 14 days after pesticide application, and the bait-lamina test. Field tests assessed organic matter breakdown using the litterbag and bait-lamina assays. Earthworm populations in the field were assessed using formalin application and hand-sorting. The neutral red retention time and comet assays were sensitive biomarkers to the effects of chlorpyrifos on the earthworm E. andrei; however, the earthworm avoidance test was not sufficiently robust to assess these effects. Feeding activity of soil biota, assessed by the bait lamina test, was significantly inhibited by chlorpyrifos after 97 days, but recovered by the 118th day of the test. Litterbag test showed no significant differences in comparison to controls. Earthworm abundance in the fi eld was too low to adequately test the sensitivity of this assessment endpoint.

Registro:

Documento: Artículo
Título:Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters
Autor:Piola, L.; Fuchs, J.; Oneto, M.L.; Basack, S.; Giménez, R.; Massaro, R.; Papa, J.C.; Kesten, E.; Casabé, N.
Filiación:Universidad de Buenos Aires (UBA), Fac. de Ciencias Exactas y Naturales (FCEN), Depart. de Química Biológica (DQB), Toxicología y Química Legal, Ciudad Universitaria, Pabellón 2, 4 piso, 1428 Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Toxicología y Química Legal, UBA, FCEN, DQB, Ciudad Universitaria, Pabellón 2, 4 piso, 1428 Buenos Aires, Argentina
UBA, Facultad de Agronomía, Area de Protección Vegetal, Avenida San Martín 4453, 1417 Buenos Aires, Argentina
Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Oliveros, Ruta 11, Km 353, 2206 Oliveros, Santa Fe, Argentina
Palabras clave:Agricultural soil; Eisenia andrei; Field assay; Laboratory bioassay; Eisenia andrei; Triticum aestivum
Año:2009
Volumen:44
Número:8
Página de inicio:874
Página de fin:880
DOI: http://dx.doi.org/10.1590/S0100-204X2009000800011
Título revista:Pesquisa Agropecuaria Brasileira
Título revista abreviado:Pesqui. Agropecu. Bras.
ISSN:0100204X
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0100204X_v44_n8_p874_Piola.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100204X_v44_n8_p874_Piola

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Citas:

---------- APA ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Giménez, R., Massaro, R., Papa, J.C.,..., Casabé, N. (2009) . Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters . Pesquisa Agropecuaria Brasileira, 44(8), 874-880.
http://dx.doi.org/10.1590/S0100-204X2009000800011
---------- CHICAGO ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Giménez, R., Massaro, R., et al. "Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters " . Pesquisa Agropecuaria Brasileira 44, no. 8 (2009) : 874-880.
http://dx.doi.org/10.1590/S0100-204X2009000800011
---------- MLA ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Giménez, R., Massaro, R., et al. "Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters " . Pesquisa Agropecuaria Brasileira, vol. 44, no. 8, 2009, pp. 874-880.
http://dx.doi.org/10.1590/S0100-204X2009000800011
---------- VANCOUVER ----------
Piola, L., Fuchs, J., Oneto, M.L., Basack, S., Giménez, R., Massaro, R., et al. Biomarkers for the assessment of chlorpyrifos effects on earthworms and on soil functional parameters . Pesqui. Agropecu. Bras. 2009;44(8):874-880.
http://dx.doi.org/10.1590/S0100-204X2009000800011