Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid-Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana. © 2009 Embrapa Informação Tecnológica.

Registro:

Documento: Artículo
Título:Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana
Autor:Cangahuala-Inocente, G.C.; Steiner, N.; Maldonado, S.B.; Guerra, M.P.
Filiación:Univ. Federal de Santa Catarina, Laboratório de Fisiologia do, Desenvolvimento e Genética Vegetal, Programa de Pós-graduaçao em Recursos, Genéticos Vegetais, Rodovia Admar Gonzaga, n 1346, Bairro Itacorubi, CEP 88034-001 Florianópolis, SC, Brazil
Universidad de Buenos Aires, Departamento de Biodiversidad y Biología Experimental, Laboratory 17. Pabellón 2, Ciudad Universitaria, C1428EHA, Ciudad de Buenos Aires, Argentina
Palabras clave:Metabolism; Pineapple guava; Starch; Total sugars; Feijoa; Feijoa sellowiana
Año:2009
Volumen:44
Número:3
Página de inicio:217
Página de fin:224
DOI: http://dx.doi.org/10.1590/S0100-204X2009000300001
Título revista:Pesquisa Agropecuaria Brasileira
Título revista abreviado:Pesqui. Agropecu. Bras.
ISSN:0100204X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0100204X_v44_n3_p217_CangahualaInocente

Referencias:

  • Bandyopadhyay, S., Hamill, J.D., Ultrastructural studies of somatic embryos of Eucalyptus nitens and comparisons with zygotic embryos found in mature seeds (2000) Annals of Botany, 86, pp. 237-244
  • Borisjuk, L., Rolletschek, H., Radchuk, R., Weschke, W., Wobus, U., Weber, H., Seed development and differentiation: a role for metabolic regulation (2004) Plant Biology, 6, pp. 375-386
  • Bradford, M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Analytical Biochemistry, 72, pp. 248-254
  • Brownfield, D.L., Todd, C.D., Stone, S.L., Deyholos, M.K., Gifford, D.J., Patterns of storage protein and triacylglycerol accumulation during loblolly pine somatic embryo maturation (2007) Plant Cell Tissue and Organ Culture, 88, pp. 217-223
  • Cangahuala-inocente, G.C., Dal vesco, L.L., Steinmacher, D., Torres, A.C., Guerra, M.P., Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg) Burret): induction, conversion and synthetic seeds (2007) Scientia Horticulturae, 111, pp. 228-234
  • Cangahuala-inocente, G.C., Steiner, N., Santos, M., Guerra, M.P., Morphohistological analysis and histochemistry of Feijoa sellowiana somatic embryogenesis (2004) Protoplasma, 224, pp. 33-40
  • Cangahuala-inocente, G.C., Villarino, A., Seixa, D., Dumas-gaudot, E., Terenzi, H., Guerra, M.P., Differential proteomic analysis of developmental stages of Acca sellowiana somatic embryos (2009) Acta Physiologiae Plantarum, 33, pp. 45-53
  • Canhoto, J.M., Cruz, G.S., Feijoa sellowiana Berg (Pineapple guava) (1996) Trees IV, pp. 156-172. , In: Bajaj, Y.P.S. (Ed.). Berlin: Springer Verlag. (Biotechnology in Agriculture and Forestry, 35)
  • Dal vesco, L.L., Guerra, M.P., The effectiveness of nitrogen sources in Acca (Feijoa sellowiana Berg) somatic embryogenesis (2001) Plant Cell, Tissue and Organ Culture, 64, pp. 19-25
  • Fehér, A., Why somatic plant cells start to form embryos? (2006) Somatic embryogenesis, p. 357. , In: Mujib, A.; Šamaj, J. (Ed.). Berlin: Springer (Plant Cell Monographs, 2)
  • Fehér, A., Pasternak, T.P., Dudits, D., Transition of somatic plant cells to an embryogenic state (2003) Plant Cell, Tissue and Organ Culture, 74, pp. 201-228
  • Gahan, P.B., (1984) Plant histochemistry and cytochemistry: an introduction, p. 301. , London: Academic Press
  • Gibson, S.I., Control of plant development and gene expression by sugar signaling (2005) Current Opinion in Plant Biology, 8, pp. 93-102
  • Gomes, F.L.A.F., Heredia, F.F., Silva, P.B.e., Faco, O., Campos, F.A.P., Somatic embryogenesis and plant regeneration in Opuntia ficus-indica(L.) Mill (Cactaceae) (2006) Scientia Horticulturae, 108, pp. 15-21
  • Griga, M., Horáček, J., Klenotičová, H., Protein patterns associated with Pisum sativumsomatic embryogenesis (2007) Biologia Plantarum, 51, pp. 201-211
  • Guerra, M.P., Dal vesco, L.L., Ducroquet, J.P.H.J., Nodari, R.O., Reis, M.S.dos., Somatic embryogenesis in goiabeira serrana: genotype response, auxinic shock and synthetic seeds (2001) Revista Brasileira de Fisiologia Vegetal, 13, pp. 117-128
  • Jiménez, V.M., Regulation of in vitro somatic embryogenesis with emphasis on the role of endogenous hormones (2001) Revista Brasileira de Fisiologia Vegetal, 13, pp. 196-223
  • Lipavská, H., Konrádová, H., Somatic embryogenesis in conifers: the role of carbohydrate metabolism (2004) In Vitro Cellular and Development Biology Plant, 40, pp. 23-30
  • Lulsdorf, M.M., Tautorus, T.E., Kikcio, S.I., Dunstan, D.I., Growth parameters of embryogenic suspension culture of interior spruce (Picea glauca-engelmannii complex) and black spruce (Picea mariana Mill.) (1992) Plant Science, 82, pp. 227-234
  • Martin, A.B., Cuadrado, Y., Guerra, H., Gallego, P., Hita, O., Martin, L., Dorado, A., Villalobos, N., Differences in the contents of total sugars, reducing sugars, starch and sucrose in embryogenic and non-embryogenic calli from Medicago arborea L (2000) Plant Science, 154, pp. 143-151
  • Mccready, R.M., Guggolz, J., Owens, H.S., Determination of starch and amylose in vegetables (1950) Analytical Chemistry, 22, pp. 1156-1158
  • Merkle, S.A., Parrott, W.A., Flinn, B.S., Morphogenic aspects of somatic embryogenesis (1995) In vitro embryogenesis in plants, pp. 155-203. , In: Thorpe, T.A. (Ed.). Dordrecht: Kluwer Academic
  • Misra, S., Attree, S.M., Leal, I., Fowke, L.C., Effect of abscisic acid, osmoticum, and desiccation on synthesis of storage proteins during the development of white spruce somatic embryos (1993) Annals of Botany, 71, pp. 11-22
  • Morcillo, F., Aberlenc-bertossi, F., Noirot, M., Hamon, S., Duval, Y., Differential effect of glutamine and arginine on 7S globulin accumulation during the maturation of oil palm somatic embryos (1999) Plant Cell Reports, 18, pp. 868-872
  • Morel, G.M., Wetmore, R.H., Tissue culture of monocotyledons (1951) American Journal of Botany, 38, pp. 138-140
  • Nieves, N., Sagarra, F., González, R., Lezcano, Y., Cid, M., Blanco, M.A., Castillo, R., Effect of exogenous arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and the contents of the soluble proteins and praline (2008) Plant Cell, Tissue and Organ Culture, 95, pp. 313-320
  • Nomura, K., Komamine, A., Physiological and biochemical aspects of somatic embryogenesis (1995) In vitro embryogenesis in plants, pp. 249-266. , In: Thorpe, T.A. (Ed.). Dordrecht: Kluwer Academic
  • O'Brien, T.P., Feder, N., Mccully, M.E., Polychromatic staining of plant cell walls by toluidine blue O (1964) Protoplasma, 59, pp. 368-373
  • Pescador, R., Kerbauy, G.B., Kraus, J.E., de Ferreira, W.M., Guerra, M.P., de Figueiredo-Ribeiro, R.C., Changes in soluble carbohydrates and starch amounts during somatic and zygotic embryogenesis of Acca sellowiana (Myrtaceae) (2008) In Vitro Cellular and Developmental Biology-Plant, 44, pp. 289-299
  • Puigderrajols, P., Mir, G., Molinas, M., Ultrastructure of early embryogenesis by multicellular and unicellular pathways in cork oak (Quercus suber L.) (2001) Annals of Botany, 87, pp. 179-189
  • Rai, M.K., Akhtar, N., Jaiswal, V.S., Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local (2007) Scientia Horticulturae, 113, pp. 129-133
  • Sané, D., Aberlenc-bertossi, F., Gassama-dia, Y.K., Sagna, M., Trouslot, M.F., Duval, Y., Borgel, A., Histocytological analysis of callogenesis and somatic embryogenesis from cell suspensions of date palm (Phoenix dactylifera) (2006) Annals of Botany, 98, pp. 301-308
  • Shannon, J.C., A procedure for the extraction and fractionation of carbohydrates from immature Zea mays kernels (1968) Research Bulletin, 842, pp. 1-8
  • Umbreit, W.W., Burris, R.H., Stauffer, J.F., (1964) Manometric techniques, , Minneapolis: Burgess
  • Von arnold, S., Eriksson, T., In vitro studies of adventitious shoot formation in Pinus contorta (1981) Canadian Journal of Botany, 59, pp. 870-874
  • Wise, M.J., Tunnacliffe, A., POPP the question: what do LEA proteins do? (2004) Trends in Plant Science, 9, pp. 13-17

Citas:

---------- APA ----------
Cangahuala-Inocente, G.C., Steiner, N., Maldonado, S.B. & Guerra, M.P. (2009) . Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana . Pesquisa Agropecuaria Brasileira, 44(3), 217-224.
http://dx.doi.org/10.1590/S0100-204X2009000300001
---------- CHICAGO ----------
Cangahuala-Inocente, G.C., Steiner, N., Maldonado, S.B., Guerra, M.P. "Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana " . Pesquisa Agropecuaria Brasileira 44, no. 3 (2009) : 217-224.
http://dx.doi.org/10.1590/S0100-204X2009000300001
---------- MLA ----------
Cangahuala-Inocente, G.C., Steiner, N., Maldonado, S.B., Guerra, M.P. "Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana " . Pesquisa Agropecuaria Brasileira, vol. 44, no. 3, 2009, pp. 217-224.
http://dx.doi.org/10.1590/S0100-204X2009000300001
---------- VANCOUVER ----------
Cangahuala-Inocente, G.C., Steiner, N., Maldonado, S.B., Guerra, M.P. Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana . Pesqui. Agropecu. Bras. 2009;44(3):217-224.
http://dx.doi.org/10.1590/S0100-204X2009000300001