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Godoy, M.S.; Nikel, P.I.; Cabrera Gomez, J.G.; Julia Pettinari, M. "The CreC regulator of Escherichia coli, a new target for metabolic manipulations" (2016) Applied and Environmental Microbiology. 82(1):244-254
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Abstract:

The CreBC (carbon source-responsive) two-component regulation system of Escherichia coli affects a number of functions, including intermediary carbon catabolism. The impacts of different creC mutations (a ΔcreC mutant and a mutant carrying the constitutive creC510 allele) on bacterial physiology were analyzed in glucose cultures under three oxygen availability conditions. Differences in the amounts of extracellular metabolites produced were observed in the null mutant compared to the wild-type strain and the mutant carrying creC510 and shown to be affected by oxygen availability. The ΔcreC strain secreted more formate, succinate, and acetate but less lactate under low aeration. These metabolic changes were associated with differences in AckA and LdhA activities, both of which were affected by CreC. Measurement of the NAD(P)H/NAD(P)+ ratios showed that the creC510 strain had a more reduced intracellular redox state, while the opposite was observed for the ΔcreC mutant, particularly under intermediate oxygen availability conditions, indicating that CreC affects redox balance. The null mutant formed more succinate than the wild-type strain under both low aeration and no aeration. Overexpression of the genes encoding phosphoenolpyruvate carboxylase from E. coli and a NADH-forming formate dehydrogenase from Candida boidinii in the ΔcreC mutant further increased the yield of succinate on glucose. Interestingly, the elimination of ackA and adhE did not significantly improve the production of succinate. The diverse metabolic effects of this regulator on the central biochemical network of E. coli make it a good candidate for metabolic-engineering manipulations to enhance the formation of bioproducts, such as succinate. © 2015, American Society for Microbiology.

Registro:

Documento: Artículo
Título:The CreC regulator of Escherichia coli, a new target for metabolic manipulations
Autor:Godoy, M.S.; Nikel, P.I.; Cabrera Gomez, J.G.; Julia Pettinari, M.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (IQUIBICEN-CONICET), Buenos Aires, Argentina
Spanish National Centre for Biotechnology (CNB-CSIC), Madrid, Spain
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil
Palabras clave:Enzyme activity; Escherichia coli; Glucose; Metabolism; Oxygen; Biochemical network; Engineering manipulations; Extracellular metabolites; Formate dehydrogenase; Metabolic changes; Metabolic effects; Phosphoenolpyruvate carboxylase; Wild-type strain; Metabolic engineering; acetate; aeration; catabolism; coliform bacterium; metabolism; microbial community; physiology; Bacteria (microorganisms); Candida boidinii; Escherichia coli; CreC protein, E coli; Escherichia coli protein; glucose; nicotinamide adenine dinucleotide; oxygen; protein kinase; reduced nicotinamide adenine dinucleotide dehydrogenase; succinic acid; anaerobic growth; Escherichia coli; gene expression regulation; genetics; metabolic engineering; metabolism; mutation; oxidation reduction reaction; protein engineering; Anaerobiosis; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Glucose; Metabolic Engineering; Mutation; NAD; NADH Dehydrogenase; Oxidation-Reduction; Oxygen; Protein Engineering; Protein Kinases; Succinic Acid
Año:2016
Volumen:82
Número:1
Página de inicio:244
Página de fin:254
DOI: http://dx.doi.org/10.1128/AEM.02984-15
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:glucose, 50-99-7, 84778-64-3; nicotinamide adenine dinucleotide, 53-84-9; oxygen, 7782-44-7; protein kinase, 9026-43-1; reduced nicotinamide adenine dinucleotide dehydrogenase, 9027-14-9, 9032-21-7, 9079-67-8; succinic acid, 110-15-6; CreC protein, E coli; Escherichia coli Proteins; Glucose; NAD; NADH Dehydrogenase; Oxygen; Protein Kinases; Succinic Acid
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v82_n1_p244_Godoy

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Citas:

---------- APA ----------
Godoy, M.S., Nikel, P.I., Cabrera Gomez, J.G. & Julia Pettinari, M. (2016) . The CreC regulator of Escherichia coli, a new target for metabolic manipulations. Applied and Environmental Microbiology, 82(1), 244-254.
http://dx.doi.org/10.1128/AEM.02984-15
---------- CHICAGO ----------
Godoy, M.S., Nikel, P.I., Cabrera Gomez, J.G., Julia Pettinari, M. "The CreC regulator of Escherichia coli, a new target for metabolic manipulations" . Applied and Environmental Microbiology 82, no. 1 (2016) : 244-254.
http://dx.doi.org/10.1128/AEM.02984-15
---------- MLA ----------
Godoy, M.S., Nikel, P.I., Cabrera Gomez, J.G., Julia Pettinari, M. "The CreC regulator of Escherichia coli, a new target for metabolic manipulations" . Applied and Environmental Microbiology, vol. 82, no. 1, 2016, pp. 244-254.
http://dx.doi.org/10.1128/AEM.02984-15
---------- VANCOUVER ----------
Godoy, M.S., Nikel, P.I., Cabrera Gomez, J.G., Julia Pettinari, M. The CreC regulator of Escherichia coli, a new target for metabolic manipulations. Appl. Environ. Microbiol. 2016;82(1):244-254.
http://dx.doi.org/10.1128/AEM.02984-15