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Abstract:

Bioreactor cultures of Escherichia coli recombinants carrying phaBAC and phaP of Azotobacter sp. FA8 grown on glycerol under low-agitation conditions accumulated more poly(3-hydroxybutyrate) (PHB) and ethanol than at high agitation, while in glucose cultures, low agitation led to a decrease in PHB formation. Cells produced smaller amounts of acids from glycerol than from glucose. Glycerol batch cultures stirred at 125 rpm accumulated, in 24 h, 30.1% (wt/wt) PHB with a relative molecular mass of 1.9 MDa, close to that of PHB obtained using glucose. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

Registro:

Documento: Artículo
Título:Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli
Autor:De Almeida, A.; Giordano, A.M.; Nikel, P.I.; Pettinari, M.J.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, Buenos Aires, Argentina
Palabras clave:Agitation conditions; Batch culture; Bioreactor cultures; Poly(3-hydroxybutyrate); Recombinant Escherichia coli; Relative molecular mass; Batch cell culture; Cell culture; Escherichia coli; Ethanol; Glycerol; Glucose; alcohol; carboxylic acid; glucose; glycerol; hydroxybutyric acid; poly(3 hydroxybutyric acid); poly-beta-hydroxybutyrate; polyester; recombinant protein; aeration; alcohol; bioreactor; coliform bacterium; ester; glucose; recombination; aerobic metabolism; article; Azotobacter; bioreactor; chemistry; Escherichia coli; fermentation; genetics; metabolism; molecular weight; Aerobiosis; Azotobacter; Bioreactors; Carboxylic Acids; Escherichia coli; Ethanol; Fermentation; Glucose; Glycerol; Hydroxybutyrates; Molecular Weight; Polyesters; Recombinant Proteins; Azotobacter; Bacteria (microorganisms); Escherichia coli
Año:2010
Volumen:76
Número:6
Página de inicio:2036
Página de fin:2040
DOI: http://dx.doi.org/10.1128/AEM.02706-09
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:alcohol, 64-17-5; glucose, 50-99-7, 84778-64-3; glycerol, 56-81-5; hydroxybutyric acid, 1320-61-2, 35054-79-6; poly(3 hydroxybutyric acid), 26063-00-3; Carboxylic Acids; Ethanol, 64-17-5; Glucose, 50-99-7; Glycerol, 56-81-5; Hydroxybutyrates; Polyesters; Recombinant Proteins; poly-beta-hydroxybutyrate, 26063-00-3
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00992240_v76_n6_p2036_DeAlmeida.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n6_p2036_DeAlmeida

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Citas:

---------- APA ----------
De Almeida, A., Giordano, A.M., Nikel, P.I. & Pettinari, M.J. (2010) . Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli. Applied and Environmental Microbiology, 76(6), 2036-2040.
http://dx.doi.org/10.1128/AEM.02706-09
---------- CHICAGO ----------
De Almeida, A., Giordano, A.M., Nikel, P.I., Pettinari, M.J. "Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli" . Applied and Environmental Microbiology 76, no. 6 (2010) : 2036-2040.
http://dx.doi.org/10.1128/AEM.02706-09
---------- MLA ----------
De Almeida, A., Giordano, A.M., Nikel, P.I., Pettinari, M.J. "Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli" . Applied and Environmental Microbiology, vol. 76, no. 6, 2010, pp. 2036-2040.
http://dx.doi.org/10.1128/AEM.02706-09
---------- VANCOUVER ----------
De Almeida, A., Giordano, A.M., Nikel, P.I., Pettinari, M.J. Effects of aeration on the synthesis of poly(3-hydroxybutyrate) from glycerol and glucose in recombinant Escherichia coli. Appl. Environ. Microbiol. 2010;76(6):2036-2040.
http://dx.doi.org/10.1128/AEM.02706-09