Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

We have previously described a murein hydrolase activity for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356. Here we show that, in combination with nisin, this S-layer acts synergistically to inhibit the growth of pathogenic Gram-negative Salmonella enterica and potential pathogenic Gram-positive bacteria, Staphylococcus aureus and Bacillus cereus. In addition, bacteriolytic effects were observed for the Gram-positive species tested. We postulate that the S-layer enhances the access of nisin into the cell membrane by enabling it to cross the cell wall, while nisin provides the sudden ion-nonspecific dissipation of the proton motive force required to enhance the S-layer murein hydrolase activity. Copyright © 2010, American Society for Microbiology. All Rights Reserved.

Registro:

Documento: Artículo
Título:Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth
Autor:Prado-Acosta, M.; Ruzal, S.M.; Allievi, M.C.; Palomino, M.M.; Rivas, C.S.
Filiación:Universidad de Buenos Aires, Facultad Ciencias Exactas y Naturales, Departamento de Química Biológica, Buenos Aires, Argentina
Palabras clave:Bacilli; Bacillus cereus; Bacteriology; Cytology; Hydrolases; Salmonella; Bacterial growth; Gram-positive bacterium; Hydrolase activities; Lactobacillus acidophilus; Proton-motive forces; Salmonella enterica; Staphylococcus aureus; Synergistic effect; Antibiotics; bacterium; enzyme activity; growth rate; membrane; surface layer; synergism; Bacillus cereus; Bacteria (microorganisms); Lactobacillus acidophilus; Negibacteria; Posibacteria; Salmonella enterica; Staphylococcus aureus
Año:2010
Volumen:76
Número:3
Página de inicio:974
Página de fin:977
DOI: http://dx.doi.org/10.1128/AEM.01427-09
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00992240_v76_n3_p974_PradoAcosta.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v76_n3_p974_PradoAcosta

Referencias:

  • Breukink, E., Van Kraaij, C., Van Dalen, A., Demel, R.A., Siezen, R.J., De Kruijff, B., Kuipers, O.P., The orientation of nisin in membranes (1998) Biochemistry, 37, pp. 8153-8162
  • Chung, W., Hancock, R.E.W., Action of lysozyme and nisin mixtures against lactic acid bacteria (2000) Int. J. Food Microbiol., 60, pp. 25-32
  • Cleveland, J., Montville, T.J., Nes, I.F., Chikindas, M.L., Bacteriocins: Safe, natural antimicrobials for food preservation (2001) Int. J. Food Microbiol., 71, pp. 1-20
  • Datta, S., Janes, M.E., Xue, Q.G., Losso, J., La Peyre, J.F., Control of Listeria monocytogenes and Salmonella anatum on the surface of smoked salmon coated with calcium alginate coating containing oyster lysozyme and nisin (2008) J. Food Sci., 73, pp. 67-71
  • Delves-Broughton, J., Blackburn, P., Evans, R.J., Hugenholtz, J., Applications of the bacteriocin, nisin (1996) Antonie Van Leeuwenhoek, 69, p. 193202
  • Fischetti, V.A., Bacteriophage lysins as effective antibacterials (2008) Curr. Opin. Microbiol., 11, pp. 393-400
  • Gill, C.O., Holley, R.A., Surface application of lysozyme, nisin, and EDTA to inhibit spoilage and pathogenic bacteria on ham and bologna (2000) J. Food Prot., 63, pp. 1338-1346
  • Hasper, H.E., Kramer, N.E., Smith, J.L., Hillman, J.D., Zachariah, C., Kuipers, O.P., De Kruijff, B., Breukink, E., An alternative bactericidal mechanism of action for lantibiotic peptides that target lipid II (2006) Science, 313, pp. 1636-1637
  • Hyde, A.J., Parisot, J., McNichol, A., Bonev, B.B., Nisin-induced changes in Bacillus morphology suggest a paradigm of antibiotic action (2006) Proc. Natl. Acad. Sci. U. S. A., 103, pp. 19896-19901
  • Ibar, M.P., Vigo, G., Piñeyro, P., Caffer, M.I., Quiroga, P., Perfumo, C., Serovariedades de Salmonella enterica subespecie enterica en porcinos de faena y su resistencia a los antimicrobianos (2009) Rev. Argent. Microbiol., 41, pp. 156-162
  • Loeffler, J.M., Fischetti, V.A., Synergistic lethal effect of a combination of phage lytic enzymes with different activities on penicillin-sensitive and resistant streptococcus pneumoniae strains (2003) Antimicrob. Agents Chemother., 47, pp. 375-377
  • Mangalassary, S., Han, I., Rieck, J., Acton, J., Jiang, X., Sheldon, B., Dawson, P., Effect of combining nisin and/or lysozyme with in-package pasteurization on thermal inactivation of Listeria monocytogenes in ready-toeat turkey bologna (2007) J. Food Prot., 70, pp. 2503-2511
  • Murdock, C.A., Cleveland, J., Matthews, K.R., Chikindas, M.L., The synergistic effect of nisin. and lactoferrin on the Inhibition of Listeria monocytogenes and Escherichia coli O157:H7 (2007) Lett. Appl. Microbiol., (44), p. 255261
  • Nascimento, J.G., Guerreira-Pereira, M.C., Costa, S.F., São-José, C., Santos, M.A., Nisin-triggered activity of Lys44, the secreted endolysin from Oenococcus oeni phage fOg44 (2008) J. Bacteriol., 190, pp. 457-461
  • Nattress, F.M., Baker, L.P., Effects of treatment with lysozyme and nisin on the microflora and sensory properties of commercial pork (2003) Int. J. Food Microbiol., 85, pp. 259-267
  • Parisien, A., Allain, B., Zhang, J., Mandeville, R., Lan, C.Q., Novel alternatives to antibiotics: Bacteriophages, bacterial, cell wall hydrolases, and antimicrobial, peptides (2008) J. Appl. Microbiol., 104, pp. 1-13
  • Prado Acosta, M., Palomino, M.M., Allevi, M.C., Sanchez Rivas, C., Ruzal, S.M., Murein hydrolase activity in the surface layer of Lactobacillus acidophilus ATCC 4356 (2008) Appl. Environ. Microbiol., 74, pp. 7824-7827
  • Ruhr, E., Sahl, H.G., Mode of action of the peptide antibiotic nisin and influence on the membrane potential, of whole cells and on cytoplasmic and artificial membrane vesicles (1985) Antimicrob. Agents Chemother., 27, p. 841845
  • Nisin preparation: Affirmation of GRAS status as a direct human food ingredient (1988) Fed. Regist., 53, p. 11247. , U.S. Food and Drug Administration
  • Winkowski, K., Ludescher, R.T., Montville, T.J., Physiochemical characterization of the nisin-membrane interaction with liposomes derived from Listeria monocytogenes (1996) Appl. Environ. Microbiol., 62, pp. 323-327

Citas:

---------- APA ----------
Prado-Acosta, M., Ruzal, S.M., Allievi, M.C., Palomino, M.M. & Rivas, C.S. (2010) . Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth. Applied and Environmental Microbiology, 76(3), 974-977.
http://dx.doi.org/10.1128/AEM.01427-09
---------- CHICAGO ----------
Prado-Acosta, M., Ruzal, S.M., Allievi, M.C., Palomino, M.M., Rivas, C.S. "Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth" . Applied and Environmental Microbiology 76, no. 3 (2010) : 974-977.
http://dx.doi.org/10.1128/AEM.01427-09
---------- MLA ----------
Prado-Acosta, M., Ruzal, S.M., Allievi, M.C., Palomino, M.M., Rivas, C.S. "Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth" . Applied and Environmental Microbiology, vol. 76, no. 3, 2010, pp. 974-977.
http://dx.doi.org/10.1128/AEM.01427-09
---------- VANCOUVER ----------
Prado-Acosta, M., Ruzal, S.M., Allievi, M.C., Palomino, M.M., Rivas, C.S. Synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth. Appl. Environ. Microbiol. 2010;76(3):974-977.
http://dx.doi.org/10.1128/AEM.01427-09