Artículo

Arabolaza, A.; Rodriguez, E.; Altabe, S.; Alvarez, H.; Gramajo, H. "Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor" (2008) Applied and Environmental Microbiology. 74(9):2573-2582
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Abstract:

The terminal reaction in triacylglyceride (TAG) biosynthesis is the esterification of diacylglycerol (DAG) with a fatty acid molecule. To study this reaction in Streptomyces coelicolor, we analyzed three candidate genes (sco0958, sco1280, and sco0123) whose products significantly resemble the recently identified wax ester synthase/acyl-coenzyme A (CoA):DAG acyltransferase (DGAT) from Acinetobacter baylyi. The deletion of either sco0123 or sco1280 resulted in no detectable decrease in TAG accumulation. In contrast, the deletion of sco0958 produced a dramatic reduction in neutral lipid production, whereas the overexpression of this gene yielded a significant increase in de novo TAG biosynthesis. In vitro activity assays showed that Sco0958 mediates the esterification of DAG using long-chain acyl-CoAs (C14 to C 18) as acyl donors. The Km and Vmax values of this enzyme for myristoyl-CoA were 45 μM and 822 nmol mg-1 min-1, respectively. Significantly, the triple mutant strain was not completely devoid of storage lipids, indicating the existence of alternative TAG-biosynthetic routes. We present strong evidence demonstrating that the residual production of TAG in this mutant strain is mediated, at least in part, by an acyl-CoA-dependent pathway, since the triple mutant still exhibited DGAT activity. More importantly, there was substantial phospholipid:DGAT (PDAT) activity in the wild type and in the triple mutant. This is the first time that a PDAT activity has been reported for bacteria, highlighting the extreme metabolic diversity of this industrially important soil microorganism. Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Registro:

Documento: Artículo
Título:Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor
Autor:Arabolaza, A.; Rodriguez, E.; Altabe, S.; Alvarez, H.; Gramajo, H.
Filiación:Microbiology Division, Instituto de Biología Molecular Y Celular de Rosario, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina
Centro Regional de Investigación Y Desatrollo Científico Tecnológico, Facultad de Ciencias Naturales, Ciudad Universitaria 9000, San Juan Bosco Km 4, Comodoro Rivadavia (Chubut), Argentina
Microbiology Division, Facultad de Ciencias Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina
Palabras clave:Bacteria; Biosynthesis; Enzymes; Fatty acids; Genes; Reaction kinetics; Mutant strains; Streptomyces coelicolor; Glycerol; acyl coenzyme A; diacylglycerol; diacylglycerol acyltransferase 1; fatty acid; phospholipid; triacylglycerol; fatty acid; gene; lipid; metabolism; soil microorganism; Acinetobacter; Acinetobacter bayli; article; bacterial gene; bacterial metabolism; bacterium mutant; controlled study; enzyme activity; esterification; gene deletion; lipid composition; lipid storage; lipogenesis; nonhuman; sco0123 gene; sco0958 gene; sco1280 gene; Streptomyces coelicolor; Acinetobacter; Acyl Coenzyme A; Acyltransferases; Bacterial Proteins; Cloning, Molecular; Gene Deletion; Gene Dosage; Gene Expression; Kinetics; Metabolic Networks and Pathways; Recombinant Proteins; Sequence Homology, Amino Acid; Streptomyces coelicolor; Triglycerides; Acinetobacter baylyi; Streptomyces coelicolor
Año:2008
Volumen:74
Número:9
Página de inicio:2573
Página de fin:2582
DOI: http://dx.doi.org/10.1128/AEM.02638-07
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:Acyl Coenzyme A; Acyltransferases, EC 2.3.-; Bacterial Proteins; phospholipid diacylglycerol acyltransferase, EC 2.3.1.-; Recombinant Proteins; S-tetradecanoyl-coenzyme A, 3130-72-1; Triglycerides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v74_n9_p2573_Arabolaza

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Citas:

---------- APA ----------
Arabolaza, A., Rodriguez, E., Altabe, S., Alvarez, H. & Gramajo, H. (2008) . Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor. Applied and Environmental Microbiology, 74(9), 2573-2582.
http://dx.doi.org/10.1128/AEM.02638-07
---------- CHICAGO ----------
Arabolaza, A., Rodriguez, E., Altabe, S., Alvarez, H., Gramajo, H. "Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor" . Applied and Environmental Microbiology 74, no. 9 (2008) : 2573-2582.
http://dx.doi.org/10.1128/AEM.02638-07
---------- MLA ----------
Arabolaza, A., Rodriguez, E., Altabe, S., Alvarez, H., Gramajo, H. "Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor" . Applied and Environmental Microbiology, vol. 74, no. 9, 2008, pp. 2573-2582.
http://dx.doi.org/10.1128/AEM.02638-07
---------- VANCOUVER ----------
Arabolaza, A., Rodriguez, E., Altabe, S., Alvarez, H., Gramajo, H. Multiple pathways for triacylglycerol biosynthesis in Streptomyces coelicolor. Appl. Environ. Microbiol. 2008;74(9):2573-2582.
http://dx.doi.org/10.1128/AEM.02638-07