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Abstract:

We assessed the effects of different arcA mutations on poly(3- hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis was not detected in the wild-type strain in shaken flask cultures under low-oxygen conditions, while ArcA mutants gave rise to polymer accumulation of up to 24% of their cell dry weight. When grown under microaerobic conditions in a bioreactor, the arcA deletion mutant reached a PHB content of 27% ± 2%. Under the same conditions, higher biomass and PHB concentrations were observed for the strain bearing the arcA2 allele, resulting in a PHB content of 35% ± 3%. This strain grew in a simple medium at a specific growth rate of 0.69 ± 0.07 h-1, whereas the deletion mutant needed several nutritional additives and snowed a specific growth rate of 0.56 ± 0.06 h-1. The results presented here suggest that arcA mutations could play a role in heterologous PHB synthesis in microaerobiosis. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Registro:

Documento: Artículo
Título:Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis
Autor:Nikel, P.I.; Pettinari, M.J.; Galvagno, M.A.; Méndez, B.S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Biotecnológicas, Uníversidad de San Martin, Buenos Aires, Argentina
Departamento de Ingeniería Química, Facultad de Ingeniería, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria-Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Biomass; Cell culture; Escherichia coli; Mutagenesis; Strain; Synthesis (chemical); ArcA mutants; Leaky mutation; Microaerobiosis; Poly(3-hydroxybutyrate) synthesis; Organic polymers; poly(3 hydroxybutyric acid); bacteriology; gene; mutagenicity; recombination; aerobic metabolism; allele; arcA gene; arcA2 gene; article; Azotobacter; Azotobacter FA8; bacterial gene; bacterial growth; bacterial strain; biomass; bioreactor; dry weight; Escherichia coli; gene deletion; gene mutation; nonhuman; pha gene; phenotype; synthesis; wild type; Aerobiosis; Bacterial Outer Membrane Proteins; Biomass; Culture Media; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Hydroxybutyrates; Mutation; Polyesters; Recombination, Genetic; Repressor Proteins; Arca; Azotobacter; Bacteria (microorganisms); Escherichia coli
Año:2006
Volumen:72
Número:4
Página de inicio:2614
Página de fin:2620
DOI: http://dx.doi.org/10.1128/AEM.72.4.2614-2620.2006
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:poly(3 hydroxybutyric acid), 26063-00-3; arcA protein, E coli; Bacterial Outer Membrane Proteins; Culture Media; Escherichia coli Proteins; Hydroxybutyrates; poly-beta-hydroxybutyrate, 26063-00-3; Polyesters; Repressor Proteins
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00992240_v72_n4_p2614_Nikel.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v72_n4_p2614_Nikel

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Citas:

---------- APA ----------
Nikel, P.I., Pettinari, M.J., Galvagno, M.A. & Méndez, B.S. (2006) . Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis. Applied and Environmental Microbiology, 72(4), 2614-2620.
http://dx.doi.org/10.1128/AEM.72.4.2614-2620.2006
---------- CHICAGO ----------
Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S. "Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis" . Applied and Environmental Microbiology 72, no. 4 (2006) : 2614-2620.
http://dx.doi.org/10.1128/AEM.72.4.2614-2620.2006
---------- MLA ----------
Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S. "Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis" . Applied and Environmental Microbiology, vol. 72, no. 4, 2006, pp. 2614-2620.
http://dx.doi.org/10.1128/AEM.72.4.2614-2620.2006
---------- VANCOUVER ----------
Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S. Poly(3-hydroxybutyrate) synthesis by recombinant Escherichia coli arcA mutants in microaerobiosis. Appl. Environ. Microbiol. 2006;72(4):2614-2620.
http://dx.doi.org/10.1128/AEM.72.4.2614-2620.2006