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Abstract:

In the same way that cry genes, coding for larvicidal delta endotoxins, constitute a large and diverse gene family, the cyt genes for hemolytic toxins seem to compose another set of highly related genes in Bacillus thuringiensis. Although the occurrence of Cyt hemolytic factors in B. thuringiensis has been typically associated with mosquitocidal strains, we have recently shown that cyt genes are also present in strains with different pathotypes; this is the case for the morrisoni subspecies, which includes strains biologically active against dipteran, lepidopteran, and coleopteran larvae. In addition, while one Cyt type of protein has been described in all of the mosquitocidal strains studied so far, the present study confirms that at least two Cyt toxins coexist in the more toxic antidipteran strains, such as B. thuringiensis subsp. israelensis and subsp. morrisoni PG14, and that this could also be the case for many others. In fact, PCR screening and Western blot analysis of 50 B. thuringiensis strains revealed that cyt2-related genes are present in all strains with known antidipteran activity, as well as in some others with different or unknown host ranges. Partial DNA sequences for several of these genes were determined, and protein sequence alignments revealed a high degree of conservation of the structural domains. These findings point to an important biological role for Cyt toxins in the final in vivo toxic activity of many B. thuringiensis strains.

Registro:

Documento: Artículo
Título:The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family
Autor:Guerchicoff, A.; Delécluse, A.; Rubinstein, C.P.
Filiación:Fac. de Ciencias Exactas y Naturales, Depto. de Quim. Biológica, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
U. des Bacteries Entomopathogenes, Institut Pasteur, 75724 Paris Cedex 15, France
Virrey Loreto 1865, 3rd, 1426 Buenos Aires, Argentina
Palabras clave:bacterial protein; bacterial toxin; endotoxin; hemolysin; insecticidal crystal protein, Bacillus Thuringiensis; amino acid sequence; article; Bacillus thuringiensis; bacterial gene; classification; DNA sequence; genetics; metabolism; methodology; molecular genetics; multigene family; polymerase chain reaction; sequence alignment; Amino Acid Sequence; Bacillus thuringiensis; Bacterial Proteins; Bacterial Toxins; Endotoxins; Genes, Bacterial; Hemolysin Proteins; Molecular Sequence Data; Multigene Family; Polymerase Chain Reaction; Sequence Alignment; Sequence Analysis, DNA
Año:2001
Volumen:67
Número:3
Página de inicio:1090
Página de fin:1096
DOI: http://dx.doi.org/10.1128/AEM.67.3.1090-1096.2001
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:Bacterial Proteins; Bacterial Toxins; Endotoxins; Hemolysin Proteins; insecticidal crystal protein, Bacillus Thuringiensis
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00992240_v67_n3_p1090_Guerchicoff.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v67_n3_p1090_Guerchicoff

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Citas:

---------- APA ----------
Guerchicoff, A., Delécluse, A. & Rubinstein, C.P. (2001) . The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family. Applied and Environmental Microbiology, 67(3), 1090-1096.
http://dx.doi.org/10.1128/AEM.67.3.1090-1096.2001
---------- CHICAGO ----------
Guerchicoff, A., Delécluse, A., Rubinstein, C.P. "The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family" . Applied and Environmental Microbiology 67, no. 3 (2001) : 1090-1096.
http://dx.doi.org/10.1128/AEM.67.3.1090-1096.2001
---------- MLA ----------
Guerchicoff, A., Delécluse, A., Rubinstein, C.P. "The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family" . Applied and Environmental Microbiology, vol. 67, no. 3, 2001, pp. 1090-1096.
http://dx.doi.org/10.1128/AEM.67.3.1090-1096.2001
---------- VANCOUVER ----------
Guerchicoff, A., Delécluse, A., Rubinstein, C.P. The Bacillus thuringiensis cyt Genes for Hemolytic Endotoxins Constitute a Gene Family. Appl. Environ. Microbiol. 2001;67(3):1090-1096.
http://dx.doi.org/10.1128/AEM.67.3.1090-1096.2001