Artículo

Este artículo es de Acceso Abierto y puede ser descargado en su versión final desde nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Pseudomonas oleovorans GPo1 and its polyhydroxyalkanoic acid (PHA) depolymerization-minus mutant, GPo500 phaZ, residing in natural water microcosms, were utilized to asses the effect of PHA availability on survival and resistance to stress agents. The wild-type strain showed increased survival compared to the PHA depolymerase-minus strain. The appearance of a round cellular shape, characteristic of bacteria growing under starvation conditions, was delayed in the wild type in comparison to the mutant strain. Percent survival at the end of ethanol and heat challenges was always higher in GPo1 than in GPo500. Based on these results and on early experiments (H. Hippe, Arch. Mikrobiol. 56:248-277, 1967) that suggested an association of PHA utilization with respiration and oxidative phosphorylation, we investigated the association between PHA degradation and nucleotide accumulation. ATP and guanosine tetraphosphate (ppGpp) production was analyzed under culture conditions leading to PHA depolymerization. A rise in the ATP and ppGpp levels appeared concomitant with PHA degradation, while this phenomenon was not observed in the mutant strain unable to degrade the polymer. Complementation of the phaZ mutation restored the wild-type phenotype.

Registro:

Documento: Artículo
Título:Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms
Autor:Ruiz, J.A.; López, N.I.; Fernández, R.O.; Méndez, B.S.
Filiación:Depto. de Química Biologica, Facultad de Ciencias Exact. y Nat., Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:adenosine triphosphate; guanosine 3' diphosphate 5' diphosphate; nucleotide; polyhydroxyalkanoic acid; article; bacterial growth; degradation; depolymerization; gene mutation; genetic complementation; nonhuman; oxidative phosphorylation; phenotype; Pseudomonas oleovorans; stress; survival; Biodegradation, Environmental; Carboxylic Ester Hydrolases; Culture Media; Ecosystem; Ethanol; Fresh Water; Heat; Mutation; Nucleotides; Polymers; Pseudomonas; Bacteria (microorganisms); Equidae; Negibacteria; Pseudomonas; Pseudomonas oleovorans
Año:2001
Volumen:67
Número:1
Página de inicio:225
Página de fin:230
DOI: http://dx.doi.org/10.1128/AEM.67.1.225-230.2001
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:Carboxylic Ester Hydrolases, EC 3.1.1.-; Culture Media; Ethanol, 64-17-5; Nucleotides; poly(3-hydroxyalkanoic acid) depolymerase, EC 3.1.1.-; Polymers
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00992240_v67_n1_p225_Ruiz.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v67_n1_p225_Ruiz

Referencias:

  • Anderson, A.J., Dawes, E.A., Occurrence, metabolism, metabolic role, and industrial use of bacterial polyhydroxyalkanoates (1990) Microbiol. Rev., 54, pp. 450-472
  • Bochner, B.R., Ames, B.N., Complete analysis of cellular nucleotides by two-dimensional thin layer chromatography (1982) J. Biol. Chem., 257, pp. 9759-9769
  • Brandl, H., Gross, R.A., Lenz, R.W., Fuller, C., Pseudomonas oleovorans as a source of poly-β-hydroxyalkanoate for potential applications as biodegradable polyesters (1988) Appl. Environ. Microbiol., 54, pp. 1977-1982
  • Cashel, M., Rudd, K.E., The stringent response (1987), pp. 1410-1438. , J. Ingraham, K. B. Low, B. Magasanik, F. C. Neidhardt, N. Schaecter, and H. E, Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology, vol. 2. American Society for Microbiology, Washington, D.C; Cashel, M., The control of ribonucleic acid synthesis in Escherichia coli (1969) J. Biol. Chem., 244, pp. 3133-3141
  • Gengross, T.U., Reilly, P., Stubbe, J., Sinskey, A.J., Peoples, O.P., Inmunocytochemical analysis of poly-β-hydroxybutyrate PHB synthase in Alcaligenes eutrophus H16: Localization of the synthase enzyme at the surface of PHB granules (1993) J. Bacteriol., 175, pp. 5289-5293
  • Gentry, D.R., Hernández, V.J., Nguyen, L.H., Jensen, D.B., Cashel, M., Synthesis of the stationary-phase sigma factor σs is positively regulated by ppGpp (1993) J. Bacteriol., 175, pp. 7982-7989
  • Givskov, M., Eberl, L., Møller, S., Kongbak Poulsen, L., Molin, S., Responses to nutrient starvation in Pseudomonas putida KT2442: Analysis of general cross-protection, cell shape, and macromolecular content (1994) J. Bacteriol., 176, pp. 7-14
  • Híppe, H., Abbau und Wiederverwertung von poly-β-hydroxybuttersaure durch Hydrogenomonas H16 (1967) Arch. Mikrobiol., 56, pp. 248-277
  • Huisman, G.W., Wonink, E., Meima, R., Kazemier, B., Terpstra, P., Witholt, B., Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA (1991) J. Biol. Chem., 266, pp. 2191-2198
  • Huisman, G.W., Wonink, E., De Koning, G., Preusting, H., Witholt, B., Synthesis of poly(3-hydroxyalkanoates by mutant and recombinant Pseudomonas strains (1992) Appl. Microbiol. Biotechnol., 38, pp. 1-5
  • Jendrossek, D., Schirmer, A., Schlegel, H.G., Biodegradation of polyhydroxyalkanoic acids (1996) Appl. Microbiol. Biotechnol., 46, pp. 451-463
  • Kessler, B., Kraak, M.N., Ren, Q., Klinke, S., Prieto, M., Witholt, B., Enzymology and molecular genetics of PHAmc1 biosynthesis (1998), pp. 48-56. , A. Steinbüchel (ed.) Biochemical principles and mechanisms of biosynthesis and degradation of polymers. Wiley-VCH, Weinheim, Germany; Lange, R., Henger-Aronis, R., Identification of a central regulator of stationary phase gene expression in Escherichia coli (1991) Mol. Microbiol., 5, pp. 49-59
  • López, N.I., Floccari, M.E., García, A.F., Steinbüchel, A., Méndez, B.S., Effect of poly3-hydroxybutyrate content on the starvation survival of bacteria in natural waters (1995) FEMS Microbiol. Ecol., 16, pp. 95-102
  • Lowry, O.H., Rosebrough, N.J., Far, A.L., Randall, R.J., Protein measure with the Pholin-phenol reagent (1951) J. Biol. Chem., 193, pp. 265-267
  • Matin, A., Veldhuis, C., Stegeman, V., Veenhuis, M., Selective advantage of a Spirillum sp. in a carbon-limited environment. Accumulation of poly-β-hydroxybutyric acid and its role in starvation (1979) J. Gen. Microbiol., 112, pp. 349-355
  • Nystrom, T., The trials and tribulations of growth arrest (1995) Trends Microbiol., 3, pp. 131-136
  • Ostle, A., Holt, J.G., Nile Blue A as a fluorescent stain for poly-β-hydroxybutyrate (1982) Appl. Environ. Microbiol., 44, pp. 238-241
  • Postma, J., Hok-A-Hin, C.H., Shotman, J.M., Wijffelman, C.A., Van Veen, J.A., Population dynamics of Rhizobium leguminosarum Tn5 mutants with altered surface properties introduced in sterile and nonsterile soils (1991) Appl. Environ. Microbiol., 57, pp. 649-654
  • Ramos-González, M.I., Molin, S., Cloning, sequencing, and phenotypic characterization of the rpoS gene from Pseudomonas putida KT2440 (1998) J. Bacteriol., 180, pp. 3421-3431
  • Rao, N.N., Kornberg, A., Inorganic polyphosphate supports resistance and survival of stationary phase Escherichia coli (1996) J. Bacteriol., 178, pp. 1394-1400
  • Rehm, B.H.A., Krüger, N., Steinbüchel, A., A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis (1998) J. Biol. Chem., 273, pp. 24044-24051
  • Ruiz, J.A., López, N.I., Méndez, B.S., Polyhydroxyalkanoates degradation affects survival of Pseudomonas oleovorans in river water microcosms (1999) Rev. Argent. Microbiol., 31, pp. 201-204
  • Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular cloning: A laboratory manual, 2nd ed., , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y
  • Samiguet, A., Kraus, J., Henkels, M.D., Muehlchen, A.M., Loper, J.E., The sigma factor sigma S affects antibiotic production and biological control activity of Pseudomonas fluorescens Pf-5 (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 12255-12259
  • Steinbüchel, A., Schlegel, H.G., Physiology and molecular genetics of polyβ-hydroxyalkanoic acid synthesis in Alcaligenes eutrophus (1991) Mol. Microbiol., 5, pp. 535-542
  • Thomas, C.M., Smith, C.A., Incompatibility group P plasmids: Genetics, evolution and use in genetic manipulations (1987) Annu. Rev. Microbiol., 41, pp. 77-101

Citas:

---------- APA ----------
Ruiz, J.A., López, N.I., Fernández, R.O. & Méndez, B.S. (2001) . Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms. Applied and Environmental Microbiology, 67(1), 225-230.
http://dx.doi.org/10.1128/AEM.67.1.225-230.2001
---------- CHICAGO ----------
Ruiz, J.A., López, N.I., Fernández, R.O., Méndez, B.S. "Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms" . Applied and Environmental Microbiology 67, no. 1 (2001) : 225-230.
http://dx.doi.org/10.1128/AEM.67.1.225-230.2001
---------- MLA ----------
Ruiz, J.A., López, N.I., Fernández, R.O., Méndez, B.S. "Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms" . Applied and Environmental Microbiology, vol. 67, no. 1, 2001, pp. 225-230.
http://dx.doi.org/10.1128/AEM.67.1.225-230.2001
---------- VANCOUVER ----------
Ruiz, J.A., López, N.I., Fernández, R.O., Méndez, B.S. Polyhydroxyalkanoate degradation is associated with nucleotide accumulation and enhances stress resistance and survival of Pseudomonas oleovorans in natural water microcosms. Appl. Environ. Microbiol. 2001;67(1):225-230.
http://dx.doi.org/10.1128/AEM.67.1.225-230.2001