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Abstract:

We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mapping the 3' end of the Xanthomonas campestris gum operon, involved in biosynthesis of xanthan.

Registro:

Documento: Artículo
Título:New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon
Autor:Katzen, F.; Becker, A.; Ielmini, M.V.; Oddo, C.G.; Ielpi, L.
Filiación:Inst. Invest. Bioquimicas Fund. C., Fac. de Ciencias Exactas y Naturales, CONICET, 1405 Buenos Aires, Argentina
Lehrstuhl für Genetik, Universität Bielefeld, D-33501 Bielefeld, Germany
Inst. Invest. Bioquimicas Fund. C., Patricias Argentinas 435, 1405 Buenos Aires, Argentina
Palabras clave:xanthan; article; bacterium identification; biosynthesis; cloning vector; colorimetry; crossing over; gene disruption; gene mapping; gram negative bacterium; nonhuman; nucleotide sequence; operon; reverse transcription polymerase chain reaction; sequence analysis; site directed mutagenesis; xanthomonas; Base Sequence; Chromosome Mapping; DNA Primers; DNA, Bacterial; Genes, Bacterial; Genetic Vectors; Gram-Negative Bacteria; Molecular Sequence Data; Mutagenesis, Site-Directed; Operon; Reverse Transcriptase Polymerase Chain Reaction; Xanthomonas campestris; Bacteria (microorganisms); Negibacteria; Xanthomonas; Xanthomonas campestris; Xanthomonas campestris pv. campestris
Año:1999
Volumen:65
Número:1
Página de inicio:278
Página de fin:282
Título revista:Applied and Environmental Microbiology
Título revista abreviado:Appl. Environ. Microbiol.
ISSN:00992240
CODEN:AEMID
CAS:DNA Primers; DNA, Bacterial
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_00992240_v65_n1_p278_Katzen.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v65_n1_p278_Katzen

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Citas:

---------- APA ----------
Katzen, F., Becker, A., Ielmini, M.V., Oddo, C.G. & Ielpi, L. (1999) . New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon. Applied and Environmental Microbiology, 65(1), 278-282.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v65_n1_p278_Katzen [ ]
---------- CHICAGO ----------
Katzen, F., Becker, A., Ielmini, M.V., Oddo, C.G., Ielpi, L. "New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon" . Applied and Environmental Microbiology 65, no. 1 (1999) : 278-282.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v65_n1_p278_Katzen [ ]
---------- MLA ----------
Katzen, F., Becker, A., Ielmini, M.V., Oddo, C.G., Ielpi, L. "New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon" . Applied and Environmental Microbiology, vol. 65, no. 1, 1999, pp. 278-282.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v65_n1_p278_Katzen [ ]
---------- VANCOUVER ----------
Katzen, F., Becker, A., Ielmini, M.V., Oddo, C.G., Ielpi, L. New mobilizable vectors suitable for gene replacement in gram-negative bacteria and their use in mapping of the 3' end of the Xanthomonas campestris pv. campestris gum operon. Appl. Environ. Microbiol. 1999;65(1):278-282.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v65_n1_p278_Katzen [ ]