Abstract:
Bacillus subtilis 430A, isolated from the Vernonia herbacea (Vell Rusby) rhizosphere, produced an exocellular inulinase that fits the requirements for the production of syrups on an industrial scale. The partially purified enzyme, obtained by acetone precipitation, displayed a higher specificity for inulin (K(m), 8 mM) than for sucrose (56 mM) and a total invertase/total inulase ratio of 0.62. In addition, it is stable at an optimal temperature of 45 to 50°C for at least 7 h and is inhibited by the end product, fructose, at 14 mM.
Registro:
| Documento: |
Artículo
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| Título: | Characteristics of an inulinase produced by Bacillus subtilis 430A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby) |
| Autor: | Vullo, D.L.; Coto, C.E.; Sineriz, F. |
| Filiación: | Departemento Quimica Biologica, Facultad de Ciencias Exactas, Ciudad Universitaria, 4 Piso Pabellon II, 1428 Buenos Aires, Argentina
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| Palabras clave: | inulin; inulinase; article; bacillus subtilis; biosynthesis; enzyme analysis; enzyme purification; nonhuman; priority journal; temperature; Bacillus subtilis; Enzyme Stability; Insulysin; Plants; Soil Microbiology; Substrate Specificity; Support, Non-U.S. Gov't; Temperature |
| Año: | 1991
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| Volumen: | 57
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| Número: | 8
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| Página de inicio: | 2392
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| Página de fin: | 2394
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| Título revista: | Applied and Environmental Microbiology
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| Título revista abreviado: | APPL. ENVIRON. MICROBIOL.
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| ISSN: | 00992240
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| CODEN: | AEMID
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| CAS: | Insulysin, EC 3.4.24.56
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| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v57_n8_p2392_Vullo |
Citas:
---------- APA ----------
Vullo, D.L., Coto, C.E. & Sineriz, F.
(1991)
. Characteristics of an inulinase produced by Bacillus subtilis 430A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby). Applied and Environmental Microbiology, 57(8), 2392-2394.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v57_n8_p2392_Vullo [ ]
---------- CHICAGO ----------
Vullo, D.L., Coto, C.E., Sineriz, F.
"Characteristics of an inulinase produced by Bacillus subtilis 430A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby)"
. Applied and Environmental Microbiology 57, no. 8
(1991) : 2392-2394.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v57_n8_p2392_Vullo [ ]
---------- MLA ----------
Vullo, D.L., Coto, C.E., Sineriz, F.
"Characteristics of an inulinase produced by Bacillus subtilis 430A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby)"
. Applied and Environmental Microbiology, vol. 57, no. 8, 1991, pp. 2392-2394.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v57_n8_p2392_Vullo [ ]
---------- VANCOUVER ----------
Vullo, D.L., Coto, C.E., Sineriz, F. Characteristics of an inulinase produced by Bacillus subtilis 430A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby). APPL. ENVIRON. MICROBIOL. 1991;57(8):2392-2394.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00992240_v57_n8_p2392_Vullo [ ]
