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Abstract:

A novel in vivo design was used in combination with solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC/MS) to characterize the volatile compounds from the skin secretion of two species of tree frogs. Conventional SPME-GC/MS also was used for the analysis of volatiles present in skin samples and for the analysis of volatiles present in the diet and terraria. In total, 40 and 37 compounds were identified in the secretion of Hypsiboas pulchellus and H. riojanus, respectively, of which, 35 were common to both species. Aliphatic aldehydes, a low molecular weight alkadiene, an aromatic alcohol, and other aromatics, ketones, a methoxy pyrazine, sulfur containing compounds, and hemiterpenes are reported here for the first time in anurans. Most of the aliphatic compounds seem to be biosynthesized by the frogs following different metabolic pathways, whereas aromatics and monoterpenes are most likely sequestered from environmental sources. The characteristic smell of the secretion of H. pulchellus described by herpetologists as skunk-like or herbaceous is explained by a complex blend of different odoriferous components. The possible role of the volatiles found in H. pulchellus and H. riojanus is discussed in the context of previous hypotheses about the biological function of volatile secretions in frogs (e.g., sex pheromones, defense secretions against predators, mosquito repellents). © 2015, Springer Science+Business Media New York.

Registro:

Documento: Artículo
Título:Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs
Autor:Brunetti, A.E.; Merib, J.; Carasek, E.; Caramão, E.B.; Barbará, J.; Zini, C.A.; Faivovich, J.
Filiación:División Herpetología, Museo Argentino de Ciencias Naturales “Bernardino Rivadavia”-CONICET, Ángel Gallardo 470, Buenos Aires, C1405DJR, Argentina
Departamento de Química Orgánica, UMyMFOR-CONICET, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 3° piso, Buenos Aires, C1428EHA, Argentina
Departamento de Química, Universidade Federal de Santa Catarina, Campus Universitário Trindade, Florianópolis, SC 88040-900, Brazil
Laboratório de Química Analítica Ambiental e Oleoquímica, Instituto de Química, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves 9500, Porto Alegre, RS, Brazil
Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 4° piso, Buenos Aires, C1428EHA, Argentina
Palabras clave:Amphibia; Anura; Chemical defense; Glands; Hylidae; Skin; Smell; Solid phase micro extraction; volatile organic compound; analysis; animal; Anura; chemistry; female; male; mass fragmentography; metabolism; odor; skin; solid phase microextraction; species difference; Animals; Anura; Female; Gas Chromatography-Mass Spectrometry; Male; Odors; Skin; Solid Phase Microextraction; Species Specificity; Volatile Organic Compounds
Año:2015
Volumen:41
Número:4
Página de inicio:360
Página de fin:372
DOI: http://dx.doi.org/10.1007/s10886-015-0564-z
Título revista:Journal of Chemical Ecology
Título revista abreviado:J. Chem. Ecol.
ISSN:00980331
CODEN:JCECD
CAS:Volatile Organic Compounds
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00980331_v41_n4_p360_Brunetti

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Citas:

---------- APA ----------
Brunetti, A.E., Merib, J., Carasek, E., Caramão, E.B., Barbará, J., Zini, C.A. & Faivovich, J. (2015) . Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs. Journal of Chemical Ecology, 41(4), 360-372.
http://dx.doi.org/10.1007/s10886-015-0564-z
---------- CHICAGO ----------
Brunetti, A.E., Merib, J., Carasek, E., Caramão, E.B., Barbará, J., Zini, C.A., et al. "Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs" . Journal of Chemical Ecology 41, no. 4 (2015) : 360-372.
http://dx.doi.org/10.1007/s10886-015-0564-z
---------- MLA ----------
Brunetti, A.E., Merib, J., Carasek, E., Caramão, E.B., Barbará, J., Zini, C.A., et al. "Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs" . Journal of Chemical Ecology, vol. 41, no. 4, 2015, pp. 360-372.
http://dx.doi.org/10.1007/s10886-015-0564-z
---------- VANCOUVER ----------
Brunetti, A.E., Merib, J., Carasek, E., Caramão, E.B., Barbará, J., Zini, C.A., et al. Frog Volatile Compounds: Application of in vivo SPME for the Characterization of the Odorous Secretions from Two Species of Hypsiboas Treefrogs. J. Chem. Ecol. 2015;41(4):360-372.
http://dx.doi.org/10.1007/s10886-015-0564-z