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Abstract:

Compounds from the metasternal and Brindley's glands of the blood-sucking bug, Triatoma infestans, were identified by solid phase microextraction (SPME) and gas chromatography-mass spectrometry. Volatile compounds released by adult bugs during copulation or after mechanical disturbance were also characterized. Six compounds were identified and found consistently in all samples from metasternal glands. The most abundant were 3-pentanone, 2-methylbutanol, 3-pentanol, and an unidentified compound. The metasternal gland blends did not differ qualitatively between sexes. Compounds found in Brindley's glands were short chain acids, alcohols, esters, and a ketone with no qualitative differences between sexes. Isobutyric acid was the main component of this blend, and two new confirmed compounds were described as products of these glands: 2-butanone and 2-methylbutyric acid. 3-Pentanone was collected from the headspace over 33% of the copulating pairs of T. infestans. Volatiles found in the headspace of disturbed T. infestans adults included short-chain fatty acids, alcohols, esters, and ketones, with no qualitative differences between sexes. Both types of glands apparently discharge their contents after disturbance. However, most of the volatiles released by bugs after disturbance came from Brindley's glands. The locomotor activity of fourth instars increased significantly after stimulation with the odors emitted by disturbed adults, as compared with larvae stimulated by the odor of undisturbed adults or by clean air. We also studied the directional behavioral response of fifth instars to the disturbance scent in a locomotion compensator. Larvae exposed to volatiles released by disturbed adults walked away from the direction of the odor. The results suggest that this blend or part of it functions as an alarm pheromone for T. infestans. We suggest that the metasternal glands of this species are involved both in the sexual and the alarm contexts, and that the Brindley's glands probably have both alarm and defensive roles. © Springer Science + Business Media, Inc. 2006.

Registro:

Documento: Artículo
Título:Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults
Autor:Manrique, G.; Vitta, A.C.R.; Ferreira, R.A.; Zani, C.L.; Unelius, C.R.; Lazzari, C.R.; Diotaiuti, L.; Lorenzo, M.G.
Filiación:Departamento de Biodiversidad Y Biologia Experimental Laboratorio de Fisiología de Insectos, FCEyN, Universidad de Buenos Aires, Argentina
Laboratório de Triatomíneos e Epidemiologia Da Doença de Chagas-CPqRR-FIOCRUZ, Brazil
Laboratório de Química de Produtos Naturais, CPqRR-FIOCRUZ, Brazil
Department of Chemistry and Biomedical Sciences, University of Kalmar, Sweden
Institut de Recherche Sur la Biologie de l'Insecte, UMR CNRS 6035, Université François Rabelais, Tours, France
Palabras clave:Alarm pheromone; Brindley's gland; Chagas disease; Chemical communication; Male aggregation pheromone; Metasternal gland; SPME; Triatominae; insect attractants; pheromone; acid; alcohol; chemical cue; copulation; disease vector; ester; gas chromatography; hemophagy; insect; ketone; mass spectrometry; physical disturbance; volatile substance; animal; animal behavior; article; bioassay; Chagas disease; chemistry; copulation; disease carrier; female; male; mass fragmentography; metabolism; scent gland; Triatoma; volatilization; Animals; Behavior, Animal; Biological Assay; Chagas Disease; Copulation; Female; Gas Chromatography-Mass Spectrometry; Insect Vectors; Male; Pheromones; Scent Glands; Triatoma; Volatilization; Triatoma infestans
Año:2006
Volumen:32
Número:9
Página de inicio:2035
Página de fin:2052
DOI: http://dx.doi.org/10.1007/s10886-006-9127-7
Título revista:Journal of Chemical Ecology
Título revista abreviado:J. Chem. Ecol.
ISSN:00980331
CODEN:JCECD
CAS:Pheromones; insect attractants
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00980331_v32_n9_p2035_Manrique

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Citas:

---------- APA ----------
Manrique, G., Vitta, A.C.R., Ferreira, R.A., Zani, C.L., Unelius, C.R., Lazzari, C.R., Diotaiuti, L.,..., Lorenzo, M.G. (2006) . Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults. Journal of Chemical Ecology, 32(9), 2035-2052.
http://dx.doi.org/10.1007/s10886-006-9127-7
---------- CHICAGO ----------
Manrique, G., Vitta, A.C.R., Ferreira, R.A., Zani, C.L., Unelius, C.R., Lazzari, C.R., et al. "Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults" . Journal of Chemical Ecology 32, no. 9 (2006) : 2035-2052.
http://dx.doi.org/10.1007/s10886-006-9127-7
---------- MLA ----------
Manrique, G., Vitta, A.C.R., Ferreira, R.A., Zani, C.L., Unelius, C.R., Lazzari, C.R., et al. "Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults" . Journal of Chemical Ecology, vol. 32, no. 9, 2006, pp. 2035-2052.
http://dx.doi.org/10.1007/s10886-006-9127-7
---------- VANCOUVER ----------
Manrique, G., Vitta, A.C.R., Ferreira, R.A., Zani, C.L., Unelius, C.R., Lazzari, C.R., et al. Chemical communication in chagas disease vectors. Source, identity, and potential function of volatiles released by the metasternal and Brindley's glands of Triatoma infestans adults. J. Chem. Ecol. 2006;32(9):2035-2052.
http://dx.doi.org/10.1007/s10886-006-9127-7