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Abstract:

The classical three-point method for obtaining a diffusion coefficient of probes from the fluorescence recovery function in the Fluorescence Recovery After Photobleaching (FRAP) technique, when Gaussian or uniform spot-photobleaching profiles are used, leads to an expression that is not suitable for linear fittings. Therefore, determination of the diffusion coefficient is complex and very dependent on the recovery time. In this work we propose a new solution for the fluorescence recovery function after spot-bleaching using a Gaussian beam, which is a better alternative method than the three-point method for the data analysis of the FRAP kinetics. The new method can be applied for shorter recovery times thereby minimizing errors due to convective effects. It leads to a linear relationship between the recovery function and the diffusion coefficient and, as shown in the analysis of a model case, allows a more accurate determination of the diffusion coefficient of fluorescent probes. © 2008 Springer Science+Business Media, LLC.

Registro:

Documento: Artículo
Título:An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis
Autor:Frank, G.A.; Marconi, M.C.; Corti, H.R.
Filiación:Laboratorio de Electrónica Cuántica, Departamento de Física, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
NSF ERC for Extreme Ultraviolet Science and Technology, Department of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO 80525, United States
Departamento de Fisica de Materia Condensada, Centro Atómico Constituyentes, Comisión Nacional de Energía Atómica, San Martin, Buenos Aires 1650, Argentina
Instituto de Química Física de los Materiales, Ambiente y Energía (INQUIMAE), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Palabras clave:Diffusion; Fluorescence recovery; FRAP; Photobleaching
Año:2008
Volumen:37
Número:11
Página de inicio:1575
Página de fin:1591
DOI: http://dx.doi.org/10.1007/s10953-008-9330-y
Título revista:Journal of Solution Chemistry
Título revista abreviado:J. Solut. Chem.
ISSN:00959782
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00959782_v37_n11_p1575_Frank

Referencias:

  • Peters, R., Peters, J., Tews, K.H., Bähr, W., A microfluorimetric study of translational diffusion in erythrocyte membranes (1974) Biochim. Biophys. Acta Biomembr., 367, pp. 282-294
  • Jacobson, K., Wu, E., Poste, G., Measurement of the translational mobility of concanavalin A in glycerol-saline solution and on the cell surface by fluorescence recovery after photobleaching (1976) Biochim. Biophys. Acta Biomembr., 433, pp. 215-222
  • Schlessinger, J., Koppel, D.E., Axelrod, D., Jacobson, K., Webb, W.W., Elson, E.L., Lateral transport on cell membranes: Mobility of concanavalin A receptors on myoblasts (1976) Proc. Natl. Acad. Sci. USA, 73, pp. 2409-2413. , 10.1073/pnas.73.7.2409
  • Edidin, M., Zagyansky, Y., Lardner, T.J., Measurement of membrane protein lateral diffusion in single cells (1976) Science, 191, pp. 466-468. , 10.1126/science.1246629
  • Axelrod, D., Koppel, D.E., Schlessinger, J., Elson, E., Webb, W.W., Mobility measurement by analysis of fluorescence photobleaching recovery kinetics (1976) Biophys. J., 16, pp. 1055-1069
  • Koppel, D.E., Axelrod, D., Schlessinger, J., Elson, E., Webb, W.W., Dynamics of fluorescence marker concentration as a probe of mobility (1976) Biophys. J., 16, pp. 1315-1329
  • Kovaleski, J.M., Wirth, M.J., Applications of fluorescence recovery after photobleaching (1997) Anal. Chem. News Features (October 1), pp. 601A-605A
  • Mustafa, M.B., Tipton, D.L., Barkely, M.D., Russo, P.S., Dye diffusion in isotropic and liquid crystalline aqueous (hydroxypropyl)cellulose (1993) Macromolecules, 26, pp. 370-378. , 10.1021/ma00054a017
  • Champion, D., Hevert, H., Blond, G., Simatos, D., Comparison between two methods to measure translational diffusion of a small molecule at subzero temperature (1995) J. Agric. Food Chem., 43, pp. 2887-2891. , 10.1021/jf00059a022
  • Blackburn, F.R., Wang, C., Ediger, M.D., Translational and rotational motion of probes in supercooled 1,3,5-tris(naphthyl)benzene (1996) J. Phys. Chem., 100, pp. 18249-18257. , 10.1021/jp9622041
  • Champion, D., Hevert, H., Blond, G., Le Meste, M., Simatos, D., Translational diffusion in sucrose solutions in the vicinity of their glass transition temperature (1997) J. Phys. Chem. B, 101, pp. 10674-10679. , 10.1021/jp971899i
  • Rampp, M., Buttersack, C., Lüdemann, H.-D., C,T-dependence of the viscosity and the self-diffusion coefficients in some aqueous carbohydrate solutions (2000) Carbohydr. Res., 328, pp. 561-572. , 10.1016/S0008-6215(00)00141-5
  • Longinotti, M.P., Corti, H.R., Diffusion of ferrocene methanol in supercooled aqueous solutions using cylindrical microelectrodes (2006) Electrochem. Commun., 9, pp. 1444-1450. , 10.1016/j.elecom.2007.02.003
  • Magazù, S., Maisano, G., Migliardo, P., Villari, V., Experimental simulation of macromolecules in trehalose aqueous solutions: A photon correlation spectroscopy study (1999) J. Chem. Phys., 111, pp. 9086-9092. , 10.1063/1.480250
  • Eggeling, C., Widengren, J., Rigler, R., Seidel, C.A.M., Photobleaching of fluorescent dyes under conditions used for single-molecule detection: Evidence of two-step photolysis (1998) Anal. Chem., 70, pp. 2651-2659. , 10.1021/ac980027p
  • Pringsheim, P., (1949) Fluorescence and Phosphorescence, , Interscience Publishers Inc. London
  • Wells, S., Fundamentals of Fluorescence (2003) O'Brien Workshop, , Optical Microscopy in Renal Research, Indiana University-School of Medicine
  • Song, L., Hennink, E.J., Young, I.T., Tanke, H.J., Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy (1995) Biophys. J., 68, pp. 2588-2600
  • Davis, P.J., Abramowitz, M., Stegun, I.A., Gamma function and related functions (1965) Handbook of Mathematical Functions, , 4 Dover New York
  • Box, M.J., Bias in nonlinear estimation (1971) J. R. Stat. Soc. B, 33, pp. 171-201
  • Olver, F.W.J., Abramowitz, M., Stegun, I.A., Bessel functions of integer order (1965) Handbook of Mathematical Functions, , 4 Dover New York
  • Luke, Y.L., Abramowitz, M., Stegun, I.A., Integrals of Bessel functions (1965) Handbook of Mathematical Functions, , 4 Dover New York
  • Wrench, J.W., Concerning two series for the gamma function (1968) Math. Comput., 22, pp. 617-626. , 10.2307/2004538
  • Mathews, J.H., Fink, K.D., (2005) Métodos Numéricos Con Matlab, p. 393. , 3 Pearson Educación S.A. Madrid
  • Barreto, H., Maharry, D., Least median of squares and regression through the origin (2006) Comput. Stat. Data Anal., 50, pp. 1391-1397. , 10.1016/j.csda.2005.01.005
  • Saxton, M.J., Anomalous subdiffusion in fluorescence photobleaching recovery: A Monte Carlo study (2001) Biophys. J., 81, pp. 2226-2240
  • Hubert, M., Rousseeuw, P.J., Robust regression with both continuous and binary regressors (1997) J. Stat. Plann. Inf., 57, pp. 153-163. , 10.1016/S0378-3758(96)00041-9
  • Corti, H.R., Frank, G.A., Marconi, M.C., An alternative solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 2. Diffusion of fluorescein in sucrose aqueous solutions (2008) J. Solution Chem., 37. , doi: 10.1007/s10953-008-9329-4

Citas:

---------- APA ----------
Frank, G.A., Marconi, M.C. & Corti, H.R. (2008) . An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis. Journal of Solution Chemistry, 37(11), 1575-1591.
http://dx.doi.org/10.1007/s10953-008-9330-y
---------- CHICAGO ----------
Frank, G.A., Marconi, M.C., Corti, H.R. "An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis" . Journal of Solution Chemistry 37, no. 11 (2008) : 1575-1591.
http://dx.doi.org/10.1007/s10953-008-9330-y
---------- MLA ----------
Frank, G.A., Marconi, M.C., Corti, H.R. "An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis" . Journal of Solution Chemistry, vol. 37, no. 11, 2008, pp. 1575-1591.
http://dx.doi.org/10.1007/s10953-008-9330-y
---------- VANCOUVER ----------
Frank, G.A., Marconi, M.C., Corti, H.R. An alternate solution of the fluorescence recovery kinetics after spot-bleaching for measuring diffusion coefficients. 1. Theory and numerical analysis. J. Solut. Chem. 2008;37(11):1575-1591.
http://dx.doi.org/10.1007/s10953-008-9330-y