Artículo

Rondón, L.; Piuri, M.; Jacobs Jr., W.R.; De Waard, J.; Hatfull, G.F.; Takiff, H.E. "Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis" (2011) Journal of Clinical Microbiology. 49(5):1838-1842
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Abstract:

We tested a new method for detecting drug-resistant strains of Mycobacterium tuberculosis that uses a TM4 mycobacteriophage phAE87::hsp60-EGFP (EGFP-phage) engineered to contain the gene encoding enhanced green fluorescent protein (EGFP). After promising results in preliminary studies, the EGFP-phage was used to detect isoniazid (INH), rifampin (RIF), and streptomycin (STR) resistance in 155 strains of M. tuberculosis, and the results were compared to the resazurin microplate technique, with the proportion method serving as the reference standard. The resazurin technique yielded sensitivities of 94% for INH and RIF and 98% for STR and specificities of 97% for INH, 95% for RIF, and 98% for STR. The sensitivity of EGFP-phage was 94% for all three antibiotics, with specificities of 90% for INH, 93% for RIF, and 95% for STR. The EGFP-phage results were available in 2 days for RIF and STR and in 3 days for INH, with an estimated cost of ∼2$ to test the three antibiotics. Using a more stringent criterion for resistance improved the specificity of the EGFP-phage for INH and RIF without affecting the sensitivity. In preliminary studies, the EGFP-phage could also effectively detect resistance to the fluoroquinolones. The EGFP-phage method has the potential to be a valuable rapid and economic screen for detecting drug-resistant tuberculosis if the procedure can be simplified, if it can be adapted to clinical material, and if its sensitivity can be improved. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

Registro:

Documento: Artículo
Título:Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis
Autor:Rondón, L.; Piuri, M.; Jacobs Jr., W.R.; De Waard, J.; Hatfull, G.F.; Takiff, H.E.
Filiación:Laboratorio de Genética Molecular, CMBC, IVIC, Km. 11, Carr. Panamericana, Caracas 1020A, Venezuela
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, United States
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, United States
Laboratorio de Tuberculosis, Instituto de Biomedicina, Hospital Vargas, San José, Caracas, Venezuela
Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:enhanced green fluorescent protein; isoniazid; quinolone derivative; rifampicin; streptomycin; accuracy; antibiotic resistance; article; bacterial strain; bacterium detection; bacterium isolate; controlled study; intermethod comparison; mycobacteriophage; Mycobacterium bovis; Mycobacterium smegmatis; Mycobacterium tuberculosis; nonhuman; priority journal; sensitivity analysis; sensitivity and specificity; Antitubercular Agents; Drug Resistance, Bacterial; Fluorometry; Green Fluorescent Proteins; Humans; Microbial Sensitivity Tests; Microbial Viability; Mycobacteriophages; Mycobacterium tuberculosis; Sensitivity and Specificity; Staining and Labeling; Tuberculosis; Mycobacterium tuberculosis
Año:2011
Volumen:49
Número:5
Página de inicio:1838
Página de fin:1842
DOI: http://dx.doi.org/10.1128/JCM.02476-10
Título revista:Journal of Clinical Microbiology
Título revista abreviado:J. Clin. Microbiol.
ISSN:00951137
CODEN:JCMID
CAS:isoniazid, 54-85-3, 62229-51-0, 65979-32-0; rifampicin, 13292-46-1; streptomycin, 57-92-1; Antitubercular Agents; Green Fluorescent Proteins, 147336-22-9; enhanced green fluorescent protein
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00951137_v49_n5_p1838_Rondon

Referencias:

  • Albert, H., Trollip, A., Seaman, T., Mole, R.J., Simple, phage-based (FASTPplaque) technology to determine rifampicin resistance of Mycobacterium tuberculosis directly from sputum (2004) Int. J. Tuberc. Lung Dis., 8, pp. 1114-1119
  • Angeby, K.A., Klintz, L., Hoffner, S.E., Rapid and inexpensive drug susceptibility testing of Mycobacterium tuberculosis with a nitrate reductase assay (2002) J. Clin. Microbiol., 40, pp. 553-555
  • Banaiee, N., January, V., Barthus, C., Lambrick, M., RoDiti, D., Behr, M.A., Jacobs Jr., W.R., Steyn, L.M., Evaluation of a semi-automated reporter phage assay for susceptibility testing of Mycobacterium tuberculosis isolates in South Africa (2008) Tuberculosis, 88 (1), pp. 64-68. , DOI 10.1016/j.tube.2007.08.006, PII S1472979207001126
  • Boehme, C.C., Rapid molecular detection of tuberculosis and rifampin resistance N. Engl. J. Med., 363, pp. 1005-1015
  • Canetti, G., Mycobacteria: Laboratory methods for testing drug sensitivity and resistance (1963) Bull. World Health Organ., 29, pp. 565-578
  • Collins, L., Franzblau, S.G., Microplate alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium (1997) Antimicrob. Agents Chemother., 41, pp. 1004-1009
  • De Beenhouwer, H., Rapid detection of rifampicin resistance in sputum and biopsy specimens from tuberculosis patients by PCR and line probe assay (1995) Tuberc. Lung Dis., 76, pp. 425-430
  • De La Iglesia, A.I., Stella, E.J., Morbidoni, H.R., Comparison of the performances of two in-house rapid methods for antitubercular drug susceptibility testing (2009) Antimicrob. Agents Chemother., 53, pp. 808-810
  • Minion, J., Leung, E., Menzies, D., Pai, M., Microscopic-observation drug susceptibility and thin layer agar assays for the detection of drug resistant tuberculosis: A systematic review and meta-analysis (2010) Lancet Infect. Dis., 10, pp. 688-698
  • Minion, J., Pai, M., Bacteriophage assays for rifampicin resistance detection in Mycobacterium tuberculosis: Updated meta-analysis (2010) Int. J. Tuberc. Lung Dis., 14, pp. 941-951
  • Moore, D.A., Microscopic-observation drug-susceptibility assay for the diagnosis of TB (2006) N. Engl. J. Med., 355, pp. 1539-1550
  • Piuri, M., Jacobs Jr., W.R., Hatfull, G.F., Fluoromycobacteriophages for rapid, specific, and sensitive antibiotic susceptibility testing of Mycobacterium tuberculosis (2009) PLoS One, 4, pp. e4870
  • Safi, H., Allelic exchange and mutant selection demonstrate that common clinical embCAB gene mutations only modestly increase resistance to ethambutol in Mycobacterium tuberculosis (2010) Antimicrob. Agents Chemother., 54, pp. 103-108
  • Takiff, H.E., Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes and detection of quinolone resistance mutations (1994) Antimicrob. Agents Chemother., 38, pp. 773-780
  • (2010) Framework for Implementing New Tuberculosis Diagnostics, , World Health Organization. World Health Organization, Geneva, Switzerland

Citas:

---------- APA ----------
Rondón, L., Piuri, M., Jacobs Jr., W.R., De Waard, J., Hatfull, G.F. & Takiff, H.E. (2011) . Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis. Journal of Clinical Microbiology, 49(5), 1838-1842.
http://dx.doi.org/10.1128/JCM.02476-10
---------- CHICAGO ----------
Rondón, L., Piuri, M., Jacobs Jr., W.R., De Waard, J., Hatfull, G.F., Takiff, H.E. "Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis" . Journal of Clinical Microbiology 49, no. 5 (2011) : 1838-1842.
http://dx.doi.org/10.1128/JCM.02476-10
---------- MLA ----------
Rondón, L., Piuri, M., Jacobs Jr., W.R., De Waard, J., Hatfull, G.F., Takiff, H.E. "Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis" . Journal of Clinical Microbiology, vol. 49, no. 5, 2011, pp. 1838-1842.
http://dx.doi.org/10.1128/JCM.02476-10
---------- VANCOUVER ----------
Rondón, L., Piuri, M., Jacobs Jr., W.R., De Waard, J., Hatfull, G.F., Takiff, H.E. Evaluation of fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis. J. Clin. Microbiol. 2011;49(5):1838-1842.
http://dx.doi.org/10.1128/JCM.02476-10