A Trypanosoma cruzi λgt11 cDNA clone, JL5, expressed a recombinant protein which was found to react predominantly with chronic Chagas' heart disease sera. The cloned 35-residue-long peptide was identified as the carboxyl-terminal portion of a T. cruzi ribosomal P protein. The JL5 13 carboxyl-terminal residues shared a high degree of homology with the systemic lupus erythematosus (SLE) ribosomal P protein epitope. Synthetic peptides comprising the 13 (R-13), 10 (R-10), and 7 (R-7) carboxyl-terminal residues of the JL5 protein were used to study, by enzyme-linked immunosorbent assay, the specificity of the Chagas' disease anti-JL5 and SLE anti-P antibodies. The R-13 peptide defined a linear antigenic determinant of the JL5 recombinant protein. As was proved for JL5, R-13 defined antibody specificities which were significantly increased in chronic Chagas' heart disease patients. Only SLE anti-P positive sera were found to react with JL5 and R-13. Fine epitope mapping showed that Chagas' disease anti-JL5 and SLE anti-P antibodies define similar epitopes within the R-13 peptide. The binding of the SLE sera to JL5 was completely blocked by the R-13 peptide, indicating that the shared specificity between anti-JL5 and anti-P autoantibodies was exclusively limited to the conserved linear epitope(s) within the R-13 peptide. The prevalence of high anti-R-13 antibody titers in Chagas' heart disease patients supports the hypothesis that postulates the existence of autoimmune disorders in Chagas' heart disease.
Documento: | Artículo |
Título: | Major Trypanosoma cruzi antigenic determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope |
Autor: | Mesri, E.A.; Levitus, G.; Hontebeyrie-Joskowicz, M.; Dighiero, G.; Van Regenmortel, M.H.V.; Levin, M.J. |
Filiación: | INGEBI-CONICET, Obligado 2490, 1428 Buenos Aires, Argentina |
Palabras clave: | epitope; recombinant protein; ribosome protein; synthetic peptide; adult; aged; amino acid sequence; antigen binding; article; chagas disease; clinical article; enzyme linked immunosorbent assay; heart disease; human; priority journal; protozoon; systemic lupus erythematosus; trypanosoma cruzi; Amino Acid Sequence; Animal; Antigens, Protozoan; beta-Galactosidase; Chagas Cardiomyopathy; Comparative Study; Enzyme-Linked Immunosorbent Assay; Epitopes; Human; Lupus Erythematosus, Systemic; Molecular Sequence Data; Phosphoproteins; Recombinant Proteins; Sequence Homology, Nucleic Acid; Support, Non-U.S. Gov't; Trypanosoma cruzi; Protozoa; Trypanosoma; Trypanosoma cruzi |
Año: | 1990 |
Volumen: | 28 |
Número: | 6 |
Página de inicio: | 1219 |
Página de fin: | 1224 |
Título revista: | Journal of Clinical Microbiology |
Título revista abreviado: | J. CLIN. MICROBIOL. |
ISSN: | 00951137 |
CODEN: | JCMID |
CAS: | Antigens, Protozoan; beta-Galactosidase, EC 3.2.1.23; Epitopes; phosphoprotein P1; Phosphoproteins; Recombinant Proteins |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00951137_v28_n6_p1219_Mesri |