Artículo
Abstract:
A Trypanosoma cruzi λgt11 cDNA clone, JL5, expressed a recombinant protein which was found to react predominantly with chronic Chagas' heart disease sera. The cloned 35-residue-long peptide was identified as the carboxyl-terminal portion of a T. cruzi ribosomal P protein. The JL5 13 carboxyl-terminal residues shared a high degree of homology with the systemic lupus erythematosus (SLE) ribosomal P protein epitope. Synthetic peptides comprising the 13 (R-13), 10 (R-10), and 7 (R-7) carboxyl-terminal residues of the JL5 protein were used to study, by enzyme-linked immunosorbent assay, the specificity of the Chagas' disease anti-JL5 and SLE anti-P antibodies. The R-13 peptide defined a linear antigenic determinant of the JL5 recombinant protein. As was proved for JL5, R-13 defined antibody specificities which were significantly increased in chronic Chagas' heart disease patients. Only SLE anti-P positive sera were found to react with JL5 and R-13. Fine epitope mapping showed that Chagas' disease anti-JL5 and SLE anti-P antibodies define similar epitopes within the R-13 peptide. The binding of the SLE sera to JL5 was completely blocked by the R-13 peptide, indicating that the shared specificity between anti-JL5 and anti-P autoantibodies was exclusively limited to the conserved linear epitope(s) within the R-13 peptide. The prevalence of high anti-R-13 antibody titers in Chagas' heart disease patients supports the hypothesis that postulates the existence of autoimmune disorders in Chagas' heart disease.
Registro:
| Documento: | Artículo |
| Título: | Major Trypanosoma cruzi antigenic determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope |
| Autor: | Mesri, E.A.; Levitus, G.; Hontebeyrie-Joskowicz, M.; Dighiero, G.; Van Regenmortel, M.H.V.; Levin, M.J. |
| Filiación: | INGEBI-CONICET, Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | epitope; recombinant protein; ribosome protein; synthetic peptide; adult; aged; amino acid sequence; antigen binding; article; chagas disease; clinical article; enzyme linked immunosorbent assay; heart disease; human; priority journal; protozoon; systemic lupus erythematosus; trypanosoma cruzi; Amino Acid Sequence; Animal; Antigens, Protozoan; beta-Galactosidase; Chagas Cardiomyopathy; Comparative Study; Enzyme-Linked Immunosorbent Assay; Epitopes; Human; Lupus Erythematosus, Systemic; Molecular Sequence Data; Phosphoproteins; Recombinant Proteins; Sequence Homology, Nucleic Acid; Support, Non-U.S. Gov't; Trypanosoma cruzi; Protozoa; Trypanosoma; Trypanosoma cruzi |
| Año: | 1990 |
| Volumen: | 28 |
| Número: | 6 |
| Página de inicio: | 1219 |
| Página de fin: | 1224 |
| Título revista: | Journal of Clinical Microbiology |
| Título revista abreviado: | J. CLIN. MICROBIOL. |
| ISSN: | 00951137 |
| CODEN: | JCMID |
| CAS: | Antigens, Protozoan; beta-Galactosidase, EC 3.2.1.23; Epitopes; phosphoprotein P1; Phosphoproteins; Recombinant Proteins |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00951137_v28_n6_p1219_Mesri |
Citas:
---------- APA ----------
Mesri, E.A., Levitus, G., Hontebeyrie-Joskowicz, M., Dighiero, G., Van Regenmortel, M.H.V. & Levin, M.J. (1990) . Major Trypanosoma cruzi antigenic determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope. Journal of Clinical Microbiology, 28(6), 1219-1224.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00951137_v28_n6_p1219_Mesri [ ]
---------- CHICAGO ----------
Mesri, E.A., Levitus, G., Hontebeyrie-Joskowicz, M., Dighiero, G., Van Regenmortel, M.H.V., Levin, M.J. "Major Trypanosoma cruzi antigenic determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope" . Journal of Clinical Microbiology 28, no. 6 (1990) : 1219-1224.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00951137_v28_n6_p1219_Mesri [ ]
---------- MLA ----------
Mesri, E.A., Levitus, G., Hontebeyrie-Joskowicz, M., Dighiero, G., Van Regenmortel, M.H.V., Levin, M.J. "Major Trypanosoma cruzi antigenic determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope" . Journal of Clinical Microbiology, vol. 28, no. 6, 1990, pp. 1219-1224.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00951137_v28_n6_p1219_Mesri [ ]
---------- VANCOUVER ----------
Mesri, E.A., Levitus, G., Hontebeyrie-Joskowicz, M., Dighiero, G., Van Regenmortel, M.H.V., Levin, M.J. Major Trypanosoma cruzi antigenic determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope. J. CLIN. MICROBIOL. 1990;28(6):1219-1224.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00951137_v28_n6_p1219_Mesri [ ]
