Artículo

Alessio, A.P.; Fili, A.E.; Garrels, W.; Forcato, D.O.; Olmos Nicotra, M.F.; Liaudat, A.C.; Bevacqua, R.J.; Savy, V.; Hiriart, M.I.; Talluri, T.R.; Owens, J.B.; Ivics, Z.; Salamone, D.F.; Moisyadi, S.; Kues, W.A.; Bosch, P. "Establishment of cell-based transposon-mediated transgenesis in cattle" (2016) Theriogenology. 85(7):1297-1311.e2
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Abstract:

Transposon-mediated transgenesis is a well-established tool for genome modification in small animal models. However, translation of this active transgenic method to large animals warrants further investigations. Here, the piggyBac (PB) and sleeping beauty (SB) transposon systems were assessed for stable gene transfer into the cattle genome. Bovine fibroblasts were transfected either with a helper-independent PB system or a binary SB system. Both transposons were highly active in bovine cells increasing the efficiency of DNA integration up to 88 times over basal nonfacilitated integrations in a colony formation assay. SB transposase catalyzed multiplex transgene integrations in fibroblast cells transfected with the helper vector and two donor vectors carrying different transgenes (fluorophore and neomycin resistance). Stably transfected fibroblasts were used for SCNT and on in vitro embryo culture, morphologically normal blastocysts that expressed the fluorophore were obtained with both transposon systems. The data indicate that transposition is a feasible approach for genetic engineering in the cattle genome. © 2016 Elsevier Inc.

Registro:

Documento: Artículo
Título:Establishment of cell-based transposon-mediated transgenesis in cattle
Autor:Alessio, A.P.; Fili, A.E.; Garrels, W.; Forcato, D.O.; Olmos Nicotra, M.F.; Liaudat, A.C.; Bevacqua, R.J.; Savy, V.; Hiriart, M.I.; Talluri, T.R.; Owens, J.B.; Ivics, Z.; Salamone, D.F.; Moisyadi, S.; Kues, W.A.; Bosch, P.
Filiación:Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina
Department of Biotechnology, Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany
Laboratorio de Biotecnología Animal, Departamento de Producción Animal, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina
Department of Anatomy, Biochemistry and Physiology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, United States
Division of Medical Biotechnology, Paul-Ehrlich-Institute, Langen, Germany
Hannover Medical School, University of Hannover, Hannover, Germany
National Research Center on Equines, Bikaner, India
Palabras clave:Cattle; PiggyBac; Sleeping beauty; Transgenesis; Transposon; transposase; transposon; animal; bovine; cell line; cell nucleus transplantation; fibroblast; gene vector; genetic transfection; genetics; transgenic animal; transposon; Animals; Animals, Genetically Modified; Cattle; Cell Line; DNA Transposable Elements; Fibroblasts; Genetic Vectors; Nuclear Transfer Techniques; Transfection; Transposases
Año:2016
Volumen:85
Número:7
Página de inicio:1297
Página de fin:1311.e2
DOI: http://dx.doi.org/10.1016/j.theriogenology.2015.12.016
Título revista:Theriogenology
Título revista abreviado:Theriogenology
ISSN:0093691X
CODEN:THGNB
CAS:DNA Transposable Elements; Transposases
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0093691X_v85_n7_p1297_Alessio

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Citas:

---------- APA ----------
Alessio, A.P., Fili, A.E., Garrels, W., Forcato, D.O., Olmos Nicotra, M.F., Liaudat, A.C., Bevacqua, R.J.,..., Bosch, P. (2016) . Establishment of cell-based transposon-mediated transgenesis in cattle. Theriogenology, 85(7), 1297-1311.e2.
http://dx.doi.org/10.1016/j.theriogenology.2015.12.016
---------- CHICAGO ----------
Alessio, A.P., Fili, A.E., Garrels, W., Forcato, D.O., Olmos Nicotra, M.F., Liaudat, A.C., et al. "Establishment of cell-based transposon-mediated transgenesis in cattle" . Theriogenology 85, no. 7 (2016) : 1297-1311.e2.
http://dx.doi.org/10.1016/j.theriogenology.2015.12.016
---------- MLA ----------
Alessio, A.P., Fili, A.E., Garrels, W., Forcato, D.O., Olmos Nicotra, M.F., Liaudat, A.C., et al. "Establishment of cell-based transposon-mediated transgenesis in cattle" . Theriogenology, vol. 85, no. 7, 2016, pp. 1297-1311.e2.
http://dx.doi.org/10.1016/j.theriogenology.2015.12.016
---------- VANCOUVER ----------
Alessio, A.P., Fili, A.E., Garrels, W., Forcato, D.O., Olmos Nicotra, M.F., Liaudat, A.C., et al. Establishment of cell-based transposon-mediated transgenesis in cattle. Theriogenology. 2016;85(7):1297-1311.e2.
http://dx.doi.org/10.1016/j.theriogenology.2015.12.016