Artículo

Estamos trabajando para conseguir la versión final de este artículo
Consulte la política de Acceso Abierto del editor

Abstract:

The aim of this work was the delimitation of twelve species of the genus Ascobolus (Ascomycota, Pezizales) based on analysis of electrophoretic patterns of intracellular enzymes. Forty-seven monosporic strains and twenty-two mixed cultures of compatible strains of the genus were used and numerical techniques were applied. Six intracellular isozyme systems were analysed: aspartate aminotransferase, esterase, acid phosphatase, glutamate dehydrogenase, isocitrate dehydrogenase and superoxide dismutase. The phenograms obtained from cluster analysis (UPGMA) showed two groups of species, corresponding to the two sections of the genus (section Ascobolus and section Dasyobolus), with some differences within each section. Although high intraspecific homogeneity was observed species of Ascobolus exhibited no intraspecific variation for these enzyme systems with the exception of A. gamundiae and A. ferrugineus which were variable for esterase and acid phosphatase respectively. The results of this research allow us to establish that the twelve species studied are independent taxonomical entities.

Registro:

Documento: Artículo
Título:Isozyme analysis of twelve species of the genus Ascobolus
Autor:Dokmetzian, D.A.; Ranalli, M.E.; Saidman, B.O.
Filiación:Departamento de Biodiversidad Y Biología Experimental, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, C1428 EHA, Buenos Aires, Argentina
Palabras clave:Ascobolaceae; Fungi; Isozymes; Sexuality; Taxonomy; Ascobolaceae; Ascobolus; Ascomycota; Fungi; Pezizales
Año:2005
Volumen:92
Página de inicio:295
Página de fin:309
Título revista:Mycotaxon
Título revista abreviado:Mycotaxon
ISSN:00934666
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v92_n_p295_Dokmetzian

Referencias:

  • Backhouse, D., Willetts, H.J., Adam, P., Electrophoretic studies of Botrytis species (1984) Trans. Br. Mycol. Soc., 82, pp. 625-630
  • Beckman, L., Johnson, F.M., Esterase variation in Drosophila melanogaster (1964) Hereditas, 51, pp. 51-212
  • Bielenin, A., Jeffers, S.N., Wilcox, W.F., Jones, A.L., Separation by protein electrophoresis of six species of Phytophthora associated with deciduous fruit crops (1988) Phytopathology, 78, pp. 1402-1408
  • Bistis, G.N., Sexuality in Ascobolus stercorarius I. Morphology of the ascogonium; plasmogamy; evidence for a sexual hormonal mechanism (1956) Am. J. Bot., 43, pp. 389-394
  • Bistis, G.N., Sexuality in Ascobolus stercorarius II. Preliminary experiments on various aspects of the sexual process (1957) Am. J. Bot., 44, pp. 436-443
  • Bistis, G.N., Raper, J., Heterothallism and sexuality in Ascobolus stercorarius (1963) Am. J. Bot., 50, pp. 880-891
  • Bonde, M.R., Peterson, G.L., Dowler, W.M., A comparison of isozyme of Phakopsora pachyrhizi from the eastern hemisphere and the new world (1988) Phytopathology, 78, pp. 1491-1494
  • Boudier, J.L.E., Mémoire sur les Ascobolées (1869) Ann. Sci. Nat., 10, pp. 191-268
  • Van Brummelen, J., A world monograph of the genera Ascobolus and Saccobolus (Ascomycetes, Pezizales) (1967) Persoonia (Suppl.), 1, pp. 1-260
  • Burdon, J.J., Luig, N.H., Marshall, D.R., Isozyme uniformity and virulence variation in Puccinia graminis f. sp. tritici and P. recondita f. sp. tritici in Australia (1983) Australian J. Biol. Sci., 36, pp. 403-410
  • Burdon, J.J., Marshall, D.R., Luig, N.H., Isozyme analysis indicates that a virulent cereal rust pathogen is a somatic hybrid (1981) Nature, 293, pp. 565-566
  • Chang, Y.S., Mills, A.K., Re-examination of Psilocybe subaeruginosa and related species with comparative morphology, isozymes and mating compatibility studies (1992) Mycol. Res., 96, pp. 429-441
  • Cruickshank, R.H., Pitt, J.I., Identification of species in Penicillium subgenus penicillium by enzyme electrophoresis (1987) Mycologia, 79, pp. 614-620
  • Dessauer, H.C., Cole, C.J., Hafner, M.S., (1984) Collection and Storage of Tissues in Molecular Systematics, 588p. , D. M. Hillis and C. Moritz Eds. Sinauer Assoc. Inc. MA. USA
  • Dokmetzian, D.A., Ranalll, M.E., Ensayos de incompatibilidad e hibridación en especies de Ascobolus (1994) Physis (BA), Sec C, 49, pp. 37-44
  • Dokmetzian, D.A., Ranalll, M.E., Crecimiento de especies del género Ascobolus (Pezizales, Ascomycetes) (1998) Rev. Arg. Microbiol., 30, pp. 195-199
  • Dokmetzian, D.A., Ranalll, M.E., Taxonomía de algunas especies del género Ascobolus (Ascomycetes - Pezizales) (2001) Physis (BA) Sec C, 58, pp. 103-108
  • Dokmetzian, D.A., De Simone, S., Ranalli, M.E., Estudio sistemático y biológico de las Ascoboláceas de Argentina XVI. Un mutante natural de Ascobolus amoenus (1995) Bol. Soc. Arg. Bot., 30, pp. 231-239
  • Gamundí, I.J., Ranalli, M.E., Estudio sistemático y biológico de las Ascoboláceas de Argentina I (1964) Nova Hedwigia, 7, pp. 517-533
  • Gottlieb, A.M., Saidman, B.O., Wright, J.E., Isoenzymes of Ganoderma species from southern South America (1998) Mycol. Res., 102 (4), pp. 415-426
  • Hamrick, J.L., Lichtwardt, R.W., Lan, C.W., Levels of isozyme variation within and among Histoplasma capsulatum localities (1986) Trans. Kansas Acad. Sci., 89, pp. 49-56
  • Hanson, L.C., Wells, K., Compatibility and population structure of three species of Tremella (Heterobasidiomycetes) (1991) Mycologia, 83, pp. 273-287
  • Harrington, T.C., Steimel, J.P., Wingfield, M.J., Kile, G.A., Isozyme variation and species delimitation in the Ceratocystis coerulescens complex (1996) Mycologia, 88, pp. 104-113
  • Harris, H., Hopkinson, D.A., (1976) Handbook of Enzyme Electrophoresis in Human Genetics, , North Holland Biomedical Press, Amsterdam
  • Hepper, C.M., Sen, R., Azcon-Aguilar, C., Grace, C., Variation in certain isozymes amongst different geographical isolates of the vesicular-arbuscular mycorrhizal fungi Glomus clarum, G. monosporum and G. mosseae (1988) Soil. Biol. Biochem., 20, pp. 51-59
  • Huss, M.J., Campbell, C.L., Jennings, D.B., Leslie, J.F., Isozyme variation among biological species in the Gibberella fujikuroi species complex (Fusarium Section Liseola) (1996) Appl. Environ. Microbiol., 62, pp. 3750-3756
  • Johnson, G.B., Enzyme polymorphism and metabolism (1974) Science, 184, pp. 28-37
  • Kanapathipillai, V.S., Preliminary studies of isozyme patterns of isolates of Colletotrichum gloeosporioides from hosts plants in Malaysia (1995) Pertanika J. Trop. Agri. Sci., 18, pp. 135-139
  • Kathariou, S., (1981) Gene Pool Organization in Fusarium Moniliforme, , Ph. D. dissertation. University of California, Berkeley
  • Kerrigan, R.W., Ross, I.K., Extracellular laccases: Biochemical markers for Agaricus systematics (1988) Mycologia, 80, pp. 689-695
  • Kovach, W.L., (1993) MVSP - A Multivariate Statistical Package for IBM-PCs, Version 2. 1, , Kovach Computing Services, Pentraeth, Wales, UK
  • Maghrabi, H.A., Kish, L.P., Isozyme characterization of Ascosphaerales associated with bees. I. Ascosphaera apis, A. proliperda and A. aggregata (1985) Mycologia, 77, pp. 358-365
  • Maghrabi, H.A., Kish, L.P., Isozyme characterization of Ascosphaerales associated with bees. II. Ascosphaera major, A. atra and A. asterophora (1985) Mycologia, 77, pp. 366-372
  • Maghrabi, H.A., Kish, L.P., Isozyme characterization of Ascosphaerales associated with bees. III. Bettsia alvei (1986) Mycologia, 78, pp. 676-677
  • Maghrabi, H.A., Kish, L.P., Isozyme characterization of Ascosphaerales associated with bees. IV. Analyses (1987) Mycologia, 79, pp. 519-523
  • Manchenko, P., (1994) Handbook of Detection of Enzymes on Electrophoretic Gels, 341p. , CRC Press, Boca Raton, Florida, USA
  • May, B., Royse, D.J., Genetic variation and joint segregation of biochemical loci in the common meadow mushroom Agaricus campestris (1982) Biochem. Genet., 20, pp. 1165-1173
  • May, B., Royse, D.J., Interespecific allozyme variation within the fungal genus Pleurotus (1988) Trans. Br. Mycol. Soc., 90, pp. 29-36
  • Micales, J.A., Bonde, M.R., Peterson, G.L., Isozyme analysis in fungal taxonomy and molecular genetics (1992) Handbook of Applied Mycology, 4, pp. 57-79. , Arora, D. K. (eds.)
  • Mosa, A.A., Kobayashi, K., Ogoshi, A., Kato, M., Sato, N., Isozyme polymorphism and segregation in isolates of Phytophthora infestans from Japan (1993) Plant. Pathol., 42, pp. 26-34
  • Newton, A.C., Markers in pathogen populations (1987) Genetics and Plant Pathogenesis, pp. 187-194. , (P. R. Day and G. J. Jellis, eds.), Blackwell Scientific, Oxford
  • Newton, A.C., Caten, C.E., Johnson, R., Variation for isozymes and double-stranded RNA among isolates of Puccinia striiformis and two other cereal rusts (1985) Plant. Pathol., 34, pp. 235-247
  • Oudemans, P., Coffey, M.D., Isozyme comparison within and among worldwide sources of three morphologically distinct species of Phytophthora (1991) Mycol. Res., 95, pp. 19-30
  • Oudemans, P., Coffey, M.D., A revised systematics of twelve papillate Phytophthora species based on isozyme analysis (1991) Mycol. Res., 95, pp. 1023-1046
  • Ramos, A.M., Ranalli, M.E., Saidman, B.O., Isozyme analysis of different species of Saccobolus (2000) Mycotaxon, 74, pp. 447-462
  • Ranalli, M.E., Gamundí, I.J., Ascobolus biguttulatus sp. nov. (Ascomycetes, Pezizales). Estudios de cultivo y citología (1975) Physis (BA) Sec C, 34, pp. 1-15
  • Rayner, A.D.M., Natural genetic transfer systems in higher fungi (1990) Trans. Mycol. Soc. Jap., 31, pp. 75-87
  • Reineke, A., Zebita, C.P.W., Protein and isozyme patterns among isolates of Beauveria brogniartii with different virulence to European cockchafer larvae (Melolontha melolontha) (1996) J. Appl. Entomol., 120, pp. 307-315
  • Rinyu, E., Varga, J., Ferenczy, L., Phenotypic and genotypic analysis of variability in Aspergillus fumigatus (1995) J. Clin. Microbiol., 33, pp. 2567-2575
  • Rodrigues, K.F., Petrini, O., Leuchtmann, A., Variability among isolates of Xylaria cubensis as determined by isozyme analysis and somatic incompatibility tests (1995) Mycologia, 87, pp. 592-596
  • Rohlf, J.F., (1993) NTSYS-PC. Numerical Taxonomy and Multivariate Analysis System. Version 1.8, , Exeter Software Applied Biostatistics Inc., N. Y
  • Saidman, B.O., (1985) Estudio de la Variación Alozímica en El Género Prosopis, 231p. , Tesis para optar al título de Doctor en Ciencias Biológicas. Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires
  • Scandalios, J.G., Genetic control of multiple molecular forms of enzymes in plants, a review (1969) Biochem. Genet., 3, pp. 37-79
  • Selander, R.K., Smith, M.H., Yang, S.Y., Johnson, W.E., Gentry, J.B., Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the old-field mouse (Peromyscus polionotus) (1971) University Texas Publ., 103, pp. 49-90
  • Sneath, P.H., Sokal, R.R., (1973) Numerical Taxonomy. The Principles and Practice of Numerical Classification, 573p. , W. H. Freeman and Co., San Francisco, USA
  • Sokal, R.R., Rohlf, F.J., The comparison of dendrograms by objective methods (1962) Taxon, 11, pp. 33-40
  • Soltis, D.E., Haufler, C.H., Darrow, D.C., Gastony, G.J., Starch gel electrophoresis of Ferns. A compilation of grinding buffers, gel and electrode buffers, and staining schedules (1983) Amer. Fern J., 73, pp. 9-27
  • Vágvölgyi, C., Papp, T., Palágyi, Z., Michailides, T.J., Isozyme variation among isolates of Mucor pyriformis (1996) Mycologia, 88, pp. 602-607
  • Vallejos, C.E., Enzyme activity staining (1983) Isozymes in Plant Genetics and Breeding, pp. 469-516. , Eds. S. D. Tanksley and T. J. Orton. A: Amsterdam, Elsevier
  • Wendel, J.F., Weeden, N.F., Visualization and interpretation of plant isozymes (1989) Isozyme in Plant Biology, pp. 5-45. , D. E. Soltis and P. S. Soltis (eds.) Cap. 1
  • Wicklow, D.T., The coprophilous fungal community: A mycological system for examining ecological ideas (1981) The Fungal Community: Its Organization and Role in the Ecosystem, pp. 47-76p. , Wicklow, D. T. and Carroll, G. (eds.). Marcel Dekker, Inc, New York

Citas:

---------- APA ----------
Dokmetzian, D.A., Ranalli, M.E. & Saidman, B.O. (2005) . Isozyme analysis of twelve species of the genus Ascobolus. Mycotaxon, 92, 295-309.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v92_n_p295_Dokmetzian [ ]
---------- CHICAGO ----------
Dokmetzian, D.A., Ranalli, M.E., Saidman, B.O. "Isozyme analysis of twelve species of the genus Ascobolus" . Mycotaxon 92 (2005) : 295-309.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v92_n_p295_Dokmetzian [ ]
---------- MLA ----------
Dokmetzian, D.A., Ranalli, M.E., Saidman, B.O. "Isozyme analysis of twelve species of the genus Ascobolus" . Mycotaxon, vol. 92, 2005, pp. 295-309.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v92_n_p295_Dokmetzian [ ]
---------- VANCOUVER ----------
Dokmetzian, D.A., Ranalli, M.E., Saidman, B.O. Isozyme analysis of twelve species of the genus Ascobolus. Mycotaxon. 2005;92:295-309.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v92_n_p295_Dokmetzian [ ]