El editor permite incluir el artículo en su versión final en nuestro repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor


Twenty-six isolates obtained from soybean crops (Glycine max) with typical anthracnose symptoms were identified as Colletotrichum truncatum (73 %) and C. destructivum (26 %). Their genetic relationships were studied using the AFLP method. A UPGMA phenogram divided the strains into two clusters corresponding with the two species. Genetic distances based on association coefficient were 0.71-0.89 among the 18 C. truncatum strains and 0.67-1 among the eight C. destructivum strains. Genetic variability within species, measured in terms of percentage of polymorphic loci, was high (<90%). Only two isolates showed 100% similarity, suggesting high intraspecific variability. © 2013. Mycotaxon, Ltd.


Documento: Artículo
Título:Molecular characterization of Colletotrichum species causing soybean anthracnose in Argentina
Autor:Ramos, A.M.; Tadic, L.F.; Cinto, I.; Carmona, M.; Gally, M.
Filiación:Departamento BBE, FCEN (UBA), PROPLAME-PRHIDEB (CONICET), Argentina
Cátedra de Fitopatología, Facultad de Agronomía, UBA C.A.B.A., Argentina
Palabras clave:Genetic diversity; Glomerella glycines; Teleomorph
Página de inicio:457
Página de fin:465
Título revista:Mycotaxon
Título revista abreviado:Mycotaxon


  • Barcelos, Q.L., Souza, E.A., Damasceno E Silva, K.J., Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil (2011) Genet. Mol. Res., 10, pp. 230-242. ,
  • Briard, M., Le Clerc, V., Grzebelus, L.D., Senalik, D., Simon, P.W., Modified protocols for rapid carrot genomic DNA extraction and AFLP TM analysis using silver stain or radioisotopes (2000) Plant Molecular Biology Reporter, 18, pp. 235-241. ,
  • Carmona, M., Scandiani, M., Luque, A., Severe outbreaks of soybean frogeye leaf spot caused by Cercospora sojina in the Pampean Region, Argentina (2009) Plant Disease, 93, p. 966. ,
  • Carmona, M., Sautua, F., Reis, E.M., Prerlman, S., Gally, M., Relationship between late soybean diseases complex and rain in determining grain yield responses to fungicide applications (2011) J. Phytopathol., 159, pp. 687-693. ,
  • Correll, J.C., Morelock, T.E., Guerber, J.C., Vegetative, compatibility and virulence of the spinach anthracnose pathogen, Colletotrichum dematium (1993) Plant Disease, 77, pp. 688-691. ,
  • Correll, J.C., Guerber, J.C., Walsiwa, L.A., Sherril, J.F., Morelock, T.E., Inter and intra species variation in Colletotrichum and mechanism which affect population structure (2000) Colletotrichum: Host Specificity, Pathology, and Host-pathogen Interaction, pp. 145-179. , D Prusky et al. (eds). The American Phytopathological Society. St. Paul MN
  • Elmer, W.H., Yang, H.A., Sweetingham, M.W., Characterization of Colletotrichum gloeosporioides isolates from ornamental lupines in Connecticut (2001) Plant. Dis., 85, pp. 216-219. ,
  • Esser, K., Kuenen, R., (1967) Genetics of Fungi, ,, Springer-Verlag, New York
  • Gottlieb, A.M., Lichtwardt, R.W., Molecular variation within and among species of Harpellales (2001) Mycologia, 93, pp. 65-80. ,
  • Gottlieb, A.M., Giberti, G.C., Poggio, L., Molecular analyses of the genus Ilex (Aquifoliaceae) in southern South America, evidence from AFLP and ITS sequence data (2005) American Journal of Botany, 92, pp. 352-369. ,
  • Gower, J.C., Some distance properties of latent root and vector methods used in multivariate analysis (1966) Biometrika, 53, pp. 325-338
  • Hyde, K.D., Cai, L., McKenzie, E.H.C., Yang, Y.L., Zhang, J.Z., Prihastuti, H., Colletotrichum: A catalogue of confusion (2009) Fungal Diversity, 39, pp. 1-17
  • Hyde, K.D., Cai, L., Cannon, P.F., Crouch, J.A., Crous, P.W., Damm, U., Goodwin, P.H., Zhang, J.Z., Colletotrichum - Names in current use (2009) Fungal Diversity, 39, pp. 147-182
  • Levin, L., Ramos, A.M., Parisi, M., Gally, M., Screening of Colletotrichum (Ascomycota) isolates causal agents of soybean anthracnose, for laccase production (2007) Boletín de la Sociedad Argentina de Botánica, 42, pp. 71-77
  • McDonald, B.A., Linde, C., Pathogen population genetics, evolutionary potential, and durable resistance (2002) Ann. Rev. Phytopathol., 40, pp. 349-379
  • McDonald, B.A., McDermott, J.M., Goodwin, S.B., Allard, R.W., The population biology of hostpathogen interactions (1989) Ann. Rev. Phytopathol., 27, pp. 77-94. ,
  • Nitzan, N., Hazanovsky, M., Tal, M., Tsror, L., Vegetative compatibility groups in Colletotrichum coccodes, the causal agent of black dot on potato (2002) Phytopathology, 92, pp. 827-832. ,
  • Ramos, A.M., Forchiassin, F., Ranalli, M.E., Saidman, B., Isozyme analysis of different species of the genus Saccobolus (Ascomycetes, Pezizales) (2000) Mycotaxon, 74, pp. 447-462
  • Ramos, A.M., Gally, M., García, M.C., Levin, L., Pectinolytic enzyme production by Colletotrichum truncatum, causal agent of soybean anthracnose (2010) Rev. Iberoam. Micol., 27, pp. 186-190. ,
  • Rodríguez-Guerra, R., Ramírez-Rueda, M.T., Martínez De La Vega, O., Simpson, J., Variation in genotype, pathotype and anastomosis groups of Colletotrichum lindemuthianum isolates from Mexico (2003) Plant Pathology, 52, pp. 228-235. ,
  • Rohlf, F.J., (1993) NTSYS-PC. Numerical Taxonomy and Multivariate Analysis System Version 2.02i, , Exeter Software Applied Biostatistics Inc., New York
  • Sant'Anna, J.R., Miyamoto, C.T., Rosada, L.J., Franco, C.C.S., Kaneshima, E.N., Castro-Prado, M.A., Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis (2010) Biol. Res., 43, pp. 51-62
  • Sharma, R., Genetic differentiation of host limited forms of Colletotrichum truncatum from northwestern Himalayas (2009) Archives of Phytopathology and Plant Protection, 42, pp. 960-966. ,
  • Sneath, P.H., Sokal, R.R., (1973) Numerical Taxonomy: The Principles and Practice of Numerical Classification, , San Francisco: W.H. Feeman & Co
  • Sokal, R.R., Rohlf, J.F., The comparison of dendrograms by objective methods (1962) Taxon, 11, pp. 33-40. ,
  • Sutton, B.C., The genus Glomerella and its anamorph Colletotrichum (1992) Colletotrichum: Biology, Pathology and Control, pp. 1-26. , JA Bailey, MJ Jeger (eds). CAB International, Wallingford, UK
  • Vaillancourt, L.J., Hanau, R., Nitrate-Nonutilizing mutants used to study heterokaryosis and vegetative compatibility in Glomerella graminicola. (Colletotrichum graminicola) (1994) Exp. Mycology, 18, pp. 311-319. ,
  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., Lee, T.V., Hornes, M., Frijters, A., Zabeau, M., AFLP: A new technique for DNA fingerprinting (1995) Nucl. Acids Res., 23, pp. 4407-4414. ,
  • Wharton, P.S., Diéguez-Uribeondo, J., The biology of Colletotrichum acutatum (2004) Anales Del Jardín Botánico de Madrid, 61 (1), pp. 3-22. ,


---------- APA ----------
Ramos, A.M., Tadic, L.F., Cinto, I., Carmona, M. & Gally, M. (2013) . Molecular characterization of Colletotrichum species causing soybean anthracnose in Argentina. Mycotaxon, 123, 457-465.
---------- CHICAGO ----------
Ramos, A.M., Tadic, L.F., Cinto, I., Carmona, M., Gally, M. "Molecular characterization of Colletotrichum species causing soybean anthracnose in Argentina" . Mycotaxon 123 (2013) : 457-465.
---------- MLA ----------
Ramos, A.M., Tadic, L.F., Cinto, I., Carmona, M., Gally, M. "Molecular characterization of Colletotrichum species causing soybean anthracnose in Argentina" . Mycotaxon, vol. 123, 2013, pp. 457-465.
---------- VANCOUVER ----------
Ramos, A.M., Tadic, L.F., Cinto, I., Carmona, M., Gally, M. Molecular characterization of Colletotrichum species causing soybean anthracnose in Argentina. Mycotaxon. 2013;123:457-465.