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Abstract:

The objective of this study was to ascertain the usefulness of the AFLP technique in assessing genetic diversity among 47 strains belonging to three Ascomycota genera and as a tool for solving taxonomic problems in related morphological species. Four Msel +1 primers were assayed in combination with two EcoRI +2 and four EcoRI +3 primers. In the present study both +2 and +3 EcoRI primers were informative, but EcoRI +2 produced profiles with high complexity. The addition of the extra selective nucleotide reduced the complexity of the banding patterns generating easily readable patterns to evaluate genetic diversity within and among species. Of the three ascomycetous genera assessed in this study, Colletotrichum (Glomerellaceae) presented the highest proportion of polymorphic AFLP loci, followed in order by Iodophanus (Pezizaceae) and Saccobolus (Ascobolaceae).

Registro:

Documento: Artículo
Título:AFLP characterization in pathogenic and coprophilous fungi
Autor:Cinto, I.E.; Gottlieb, A.M.; Gally, M.; Ranalli, M.E.; Ramos, A.M.
Filiación:Departamento Biodiversidad y Biología Experimental, Lab. 9, Universidad de Buenos Aires, Int. Güiraldes 2620, C1428EHA - Buenos Aires, Argentina
LACyE, Departamento Ecología Genética y Evolución, Universidad de Buenos Aires, Int. Güiraldes 2620, C1428EHA - Buenos Aires, Argentina
Cátedra de Fitopatología, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE - Buenos Aires, Argentina
Palabras clave:Ascomycetes; Genetic characterization; Molecular markers; Taxonomy; Ascobolaceae; Ascomycota; Colletotrichum; Coprophilous; Fungi; Iodophanus; Pezizaceae; Saccobolus
Año:2009
Volumen:110
Página de inicio:81
Página de fin:87
DOI: http://dx.doi.org/10.5248/110.81
Título revista:Mycotaxon
Título revista abreviado:Mycotaxon
ISSN:00934666
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00934666_v110_n_p81_Cinto

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Citas:

---------- APA ----------
Cinto, I.E., Gottlieb, A.M., Gally, M., Ranalli, M.E. & Ramos, A.M. (2009) . AFLP characterization in pathogenic and coprophilous fungi. Mycotaxon, 110, 81-87.
http://dx.doi.org/10.5248/110.81
---------- CHICAGO ----------
Cinto, I.E., Gottlieb, A.M., Gally, M., Ranalli, M.E., Ramos, A.M. "AFLP characterization in pathogenic and coprophilous fungi" . Mycotaxon 110 (2009) : 81-87.
http://dx.doi.org/10.5248/110.81
---------- MLA ----------
Cinto, I.E., Gottlieb, A.M., Gally, M., Ranalli, M.E., Ramos, A.M. "AFLP characterization in pathogenic and coprophilous fungi" . Mycotaxon, vol. 110, 2009, pp. 81-87.
http://dx.doi.org/10.5248/110.81
---------- VANCOUVER ----------
Cinto, I.E., Gottlieb, A.M., Gally, M., Ranalli, M.E., Ramos, A.M. AFLP characterization in pathogenic and coprophilous fungi. Mycotaxon. 2009;110:81-87.
http://dx.doi.org/10.5248/110.81