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Crocker, J.; Abe, N.; Rinaldi, L.; McGregor, A.P.; Frankel, N.; Wang, S.; Alsawadi, A.; Valenti, P.; Plaza, S.; Payre, F.; Mann, R.S.; Stern, D.L. "Low affinity binding site clusters confer HOX specificity and regulatory robustness" (2015) Cell. 160(1-2):191-203
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Abstract:

In animals, Hox transcription factors define regional identity in distinct anatomical domains. How Hox genes encode this specificity is a paradox, because different Hox proteins bind with high affinity in vitro to similar DNA sequences. Here, we demonstrate that the Hox protein Ultrabithorax (Ubx) in complex with its cofactor Extradenticle (Exd) bound specifically to clusters of very low affinity sites in enhancers of the shavenbaby gene of Drosophila. These low affinity sites conferred specificity for Ubx binding in vivo, but multiple clustered sites were required for robust expression when embryos developed in variable environments. Although most individual Ubx binding sites are not evolutionarily conserved, the overall enhancer architecture - clusters of low affinity binding sites - is maintained and required for enhancer function. Natural selection therefore works at the level of the enhancer, requiring a particular density of low affinity Ubx sites to confer both specific and robust expression. © 2015 Elsevier Inc.

Registro:

Documento: Artículo
Título:Low affinity binding site clusters confer HOX specificity and regulatory robustness
Autor:Crocker, J.; Abe, N.; Rinaldi, L.; McGregor, A.P.; Frankel, N.; Wang, S.; Alsawadi, A.; Valenti, P.; Plaza, S.; Payre, F.; Mann, R.S.; Stern, D.L.
Filiación:Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, United States
Columbia University Medical Center, HHSC 1104, 701 West 168 th Street, New York, NY 10032, United States
Department of Biological and Medical Sciences, Oxford Brookes University, Gipsy Lane, Oxford, OX3 0BP, United Kingdom
Departamento de Ecología, Genética y Evolución, IEGEBA-CONICET, Universidad de Buenos Aires, Ciudad, Universitaria, Pabellón 21428, Argentina
New Jersey Neuroscience Institute, 65 James Street, Edison, NJ 08820, United States
Centre de Biologie du Développement, Université de Toulouse, UPS, Cedex 9, 31062, France
CNRS, UMR5547, Centre de Biologie du Développement, Toulouse, Cedex 9, 31062, France
Palabras clave:DNA fragment; Hox protein; DNA binding protein; Drosophila protein; extradenticle protein, Drosophila; homeodomain protein; ovo protein, Drosophila; protein binding; transcription factor; Ubx protein, Drosophila; animal cell; Article; binding affinity; binding site; chromatin immunoprecipitation; controlled study; DNA sequence; Drosophila melanogaster; Drosophila virilis; embryo; embryo cell; enhancer region; gel mobility shift assay; genetic conservation; genetic regulation; genetic variability; insect cell; natural selection; nonhuman; priority journal; reporter gene; trichome; wild type; animal; animal embryo; enhancer region; gene expression regulation; genetics; metabolism; molecular genetics; nucleotide sequence; sequence alignment; Animalia; Animals; Base Sequence; DNA-Binding Proteins; Drosophila melanogaster; Drosophila Proteins; Embryo, Nonmammalian; Enhancer Elements, Genetic; Gene Expression Regulation; Homeodomain Proteins; Molecular Sequence Data; Protein Binding; Sequence Alignment; Transcription Factors
Año:2015
Volumen:160
Número:1-2
Página de inicio:191
Página de fin:203
DOI: http://dx.doi.org/10.1016/j.cell.2014.11.041
Título revista:Cell
Título revista abreviado:Cell
ISSN:00928674
CODEN:CELLB
CAS:DNA-Binding Proteins; Drosophila Proteins; extradenticle protein, Drosophila; Homeodomain Proteins; ovo protein, Drosophila; Transcription Factors; Ubx protein, Drosophila
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00928674_v160_n1-2_p191_Crocker

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Citas:

---------- APA ----------
Crocker, J., Abe, N., Rinaldi, L., McGregor, A.P., Frankel, N., Wang, S., Alsawadi, A.,..., Stern, D.L. (2015) . Low affinity binding site clusters confer HOX specificity and regulatory robustness. Cell, 160(1-2), 191-203.
http://dx.doi.org/10.1016/j.cell.2014.11.041
---------- CHICAGO ----------
Crocker, J., Abe, N., Rinaldi, L., McGregor, A.P., Frankel, N., Wang, S., et al. "Low affinity binding site clusters confer HOX specificity and regulatory robustness" . Cell 160, no. 1-2 (2015) : 191-203.
http://dx.doi.org/10.1016/j.cell.2014.11.041
---------- MLA ----------
Crocker, J., Abe, N., Rinaldi, L., McGregor, A.P., Frankel, N., Wang, S., et al. "Low affinity binding site clusters confer HOX specificity and regulatory robustness" . Cell, vol. 160, no. 1-2, 2015, pp. 191-203.
http://dx.doi.org/10.1016/j.cell.2014.11.041
---------- VANCOUVER ----------
Crocker, J., Abe, N., Rinaldi, L., McGregor, A.P., Frankel, N., Wang, S., et al. Low affinity binding site clusters confer HOX specificity and regulatory robustness. Cell. 2015;160(1-2):191-203.
http://dx.doi.org/10.1016/j.cell.2014.11.041