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SUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO conjugase Ubc9. RSUME increases noncovalent binding of SUMO-1 to Ubc9 and enhances Ubc9 thioester formation and SUMO polymerization. RSUME enhances the sumoylation of IkB in vitro and in cultured cells, leading to an inhibition of NF-kB transcriptional activity. RSUME is induced by hypoxia and enhances the sumoylation of HIF-1α, promoting its stabilization and transcriptional activity during hypoxia. Disruption of the RWD domain structure of RSUME demonstrates that this domain is critical for RSUME action. Together, these findings point to a central role of RSUME in the regulation of sumoylation and, hence, several critical regulatory pathways in mammalian cells. © 2007 Elsevier Inc. All rights reserved.


Documento: Artículo
Título:RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia
Autor:Carbia-Nagashima, A.; Gerez, J.; Perez-Castro, C.; Paez-Pereda, M.; Silberstein, S.; Stalla, G.K.; Holsboer, F.; Arzt, E.
Filiación:Laboratorio de Fisiología y Biología Molecular, Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, C1428EHA Buenos Aires, Argentina
Max Planck Institute of Psychiatry, 80804 Munich, Germany
Affectis Pharmaceuticals, 80804 Munich, Germany
Palabras clave:CELLBIO; PROTEINS; cell protein; hypoxia inducible factor 1alpha; I kappa B; immunoglobulin enhancer binding protein; protein RSUME; protein Ubc9; SUMO 1 protein; sumo 2 protein; sumo 3 protein; SUMO protein; unclassified drug; animal cell; article; cell function; conjugation; controlled study; genetic transcription; human; human cell; hypoxia; mammal cell; mouse; nonhuman; nucleotide sequence; polymerization; priority journal; protein binding; protein domain; protein expression; protein protein interaction; protein stability; protein structure; rat; regulatory mechanism; sumoylation; Amino Acid Sequence; Animals; Base Sequence; Cell Hypoxia; Cell Line, Tumor; Cercopithecus aethiops; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; I-kappa B Kinase; Molecular Sequence Data; NF-kappa B; Organ Specificity; Protein Binding; Small Ubiquitin-Related Modifier Proteins; SUMO-1 Protein; Transcription Factors; Ubiquitin-Conjugating Enzymes; Ubiquitins; Mammalia
Página de inicio:309
Página de fin:323
Título revista:Cell
Título revista abreviado:Cell
CAS:SUMO 1 protein, 182213-10-1; Hypoxia-Inducible Factor 1, alpha Subunit; I-kappa B Kinase,; NF-kappa B; RSUME protein, human; SUMO-1 Protein; SUMO2 protein, human; SUMO3 protein, human; Small Ubiquitin-Related Modifier Proteins; Transcription Factors; Ubiquitin-Conjugating Enzymes,; Ubiquitins; ubiquitin-conjugating enzyme UBC9,


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---------- APA ----------
Carbia-Nagashima, A., Gerez, J., Perez-Castro, C., Paez-Pereda, M., Silberstein, S., Stalla, G.K., Holsboer, F.,..., Arzt, E. (2007) . RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia. Cell, 131(2), 309-323.
---------- CHICAGO ----------
Carbia-Nagashima, A., Gerez, J., Perez-Castro, C., Paez-Pereda, M., Silberstein, S., Stalla, G.K., et al. "RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia" . Cell 131, no. 2 (2007) : 309-323.
---------- MLA ----------
Carbia-Nagashima, A., Gerez, J., Perez-Castro, C., Paez-Pereda, M., Silberstein, S., Stalla, G.K., et al. "RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia" . Cell, vol. 131, no. 2, 2007, pp. 309-323.
---------- VANCOUVER ----------
Carbia-Nagashima, A., Gerez, J., Perez-Castro, C., Paez-Pereda, M., Silberstein, S., Stalla, G.K., et al. RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia. Cell. 2007;131(2):309-323.