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Abstract:

Male crabs (Chasmagnathus granulatus) exhibited a defensive response (DR) to an electric shock (8 V, 50 Hz, 1 sec). The DR so elicited was used as a model for studying the antibociceptive effect of morphine. Injections of morphine-HCl (MP) (25, 50, 100 and 150 μg/g) were administered and the DR was examined at 2, 7.5, 15, 30, 45 and 75 min post-injection. (a) MP produced a dose-dependent reduction of the crab's sensitivity to the nociceptive stimulus. (b) A 100 μg/g dose of MP caused a 50% response inhibition with an injection-shock interval of 30 minutes, but no inhibition occurred when the same dose was administered with 1.6 μg/g of naloxone-HCl, suggesting that MP acts through an opiate receptor. (c) The ED50 at 2 min post-injection was roughly 33 μg/g and the threshold dose was estimated to be 6.8 μg/g. These doses are lower than ED50 values reported for other arthropods (90 to 930 μg/g) and approach those of vertebrates. (d) The peak MP effect was reached quickly, within 2 min post-injection. The duration of the MP effect was calculated to be 45.0-75.0 min depending on the dose, and an indirect estimate of half-life elimination was 15.7 min. These values are remarkably lower than those reported for vertebrates. The shorter duration of the MP peak effect is attributable to a greater permeability of the arthropod blood-brain barrier as compared to that of verebrates. © 1988.

Registro:

Documento: Artículo
Título:Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus
Autor:Lozada, M.; Romano, A.; Maldonado, H.
Filiación:Laboratorio de Fisiologia, Comportamiento Animal Facultad de Ciencias Exactas y Naturales, UBA., Avda. Indalecio Chenaut 1910, 1426 Buenos Aires, Argentina
Palabras clave:Antinociceptive effect; Arthropods; Defensive response; Morphine pharmacokinetics; morphine; naloxone; opiate receptor; animal experiment; antinociception; arthropod; crab; defensive behavior; electric shock; invertebrate; nonhuman; priority journal; psychological aspect; Animal; Behavior, Animal; Brachyura; Dose-Response Relationship, Drug; Electroshock; Morphine; Naloxone; Reference Values; Support, Non-U.S. Gov't
Año:1988
Volumen:30
Número:3
Página de inicio:635
Página de fin:640
DOI: http://dx.doi.org/10.1016/0091-3057(88)90076-7
Título revista:Pharmacology, Biochemistry and Behavior
Título revista abreviado:Pharmacol. Biochem. Behav.
ISSN:00913057
CODEN:PBBHA
CAS:morphine, 52-26-6, 57-27-2; naloxone, 357-08-4, 465-65-6; Morphine, 57-27-2; Naloxone, 465-65-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00913057_v30_n3_p635_Lozada

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Citas:

---------- APA ----------
Lozada, M., Romano, A. & Maldonado, H. (1988) . Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus. Pharmacology, Biochemistry and Behavior, 30(3), 635-640.
http://dx.doi.org/10.1016/0091-3057(88)90076-7
---------- CHICAGO ----------
Lozada, M., Romano, A., Maldonado, H. "Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus" . Pharmacology, Biochemistry and Behavior 30, no. 3 (1988) : 635-640.
http://dx.doi.org/10.1016/0091-3057(88)90076-7
---------- MLA ----------
Lozada, M., Romano, A., Maldonado, H. "Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus" . Pharmacology, Biochemistry and Behavior, vol. 30, no. 3, 1988, pp. 635-640.
http://dx.doi.org/10.1016/0091-3057(88)90076-7
---------- VANCOUVER ----------
Lozada, M., Romano, A., Maldonado, H. Effect of morphine and naloxone on a defensive response of the crab Chasmagnathus granulatus. Pharmacol. Biochem. Behav. 1988;30(3):635-640.
http://dx.doi.org/10.1016/0091-3057(88)90076-7